Abstract
The light-harvesting complex (LHC) Ib pigment-proteins form the major component of the LHC I complex in higher plants. They comprise chlorophylls a and b, xanthophylls, and at least two polypeptide subunits of 21 and 20 kD in barley (Hordeum vulgare). We have identified two cDNA clones, LHC Ib-21 and LHC Ib-20, encoding the 21- and 20-kD LHC Ib apoproteins, respectively. N-terminal protein sequences of the purified LHC Ib polypeptides were used for the unequivocal correlation of these clones to their respective apoproteins. The cDNA clones encode two proteins that have strong sequence similarity to other LHC I and LHC II pigment-binding polypeptides of photosystems I and II. The 21-kD polypeptide contains 201 amino acid residues (22.14 kD), and the 20-kD polypeptide contains 200 amino acid residues (22.18 kD). The biogenesis of the LHC Ib apoproteins and the pigmented LHC I during the light-induced development of the chloroplast was studied. Accumulation of the two LHC Ib mRNAs is induced by light, and their amount is regulated by phytochrome. LHC Ib polypeptide accumulation in the thylakoid membrane temporally lags behind transcript accumulation. The rates of accumulation of LHC Ib transcripts and of their apoproteins lag behind those of the major LHC II component, LHC IIb. Complete assembly of the LHC Ib pigment-protein, as observed by low-temperature fluorescence spectroscopy, requires exposure of dark-grown seedlings to 72 h or more of light.
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Claims 10
Claim · model-written summary
Two cDNA clones encode the 21 and 20 kD LHC Ib apoproteins.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree1 - Gene
- LHC Ib-21 and LHC Ib-20e0
- Outcome
- encoding the 21- and 20-kD LHC Ib apoproteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“We have identified two cDNA clones, LHC Ib-21 and LHC Ib-20, encoding the 21- and 20-kD LHC Ib apoproteins, respectively”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
The encoded proteins are strongly similar to LHC I and LHC II pigment-binding polypeptides.
- Taxon
- barleyHordeum vulgaree1
- Gene
- LHC I and LHC II pigment-binding polypeptidese0
- Outcome
- strong sequence similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cDNA clones encode two proteins that have strong sequence similarity to other LHC I and LHC II pigment-binding polypeptides of photosystems I and II”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
The 21 kD polypeptide contains 201 residues and has a mass of 22.14 kD.
- Taxon
- barleyHordeum vulgaree1
- Gene
- 21-kD polypeptidee0
- Outcome
- amino acid residuese0
- Value
- 201 amino acid residuese0
- Qualifier
- 22.14 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 21-kD polypeptide contains 201 amino acid residues (22.14 kD)”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
The 20 kD polypeptide contains 200 residues and has a mass of 22.18 kD.
- Taxon
- barleyHordeum vulgaree1
- Gene
- 20-kD polypeptidee0
- Outcome
- amino acid residuese0
- Value
- 200 amino acid residuese0
- Qualifier
- 22.18 kDe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 20-kD polypeptide contains 200 amino acid residues (22.18 kD)”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
Light induced accumulation of the two LHC Ib mRNAs.
- Taxon
- barleyHordeum vulgaree1
- Treatment
- lighte0
- Gene
- LHC Ibe0
- Outcome
- Accumulation of the two LHC Ib mRNAse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of the two LHC Ib mRNAs is induced by light”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
Phytochrome regulates the amount of LHC Ib mRNA.
- Taxon
- barleyHordeum vulgaree2
- Gene
- phytochromee0
- Outcome
- amounte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their amount is regulated by phytochrome”
e1“Accumulation of the two LHC Ib mRNAs is induced by light”
e2“barley (Hordeum vulgare)”
Claim · model-written summary
LHC Ib polypeptide accumulation in thylakoids lagged behind transcript accumulation.
- Taxon
- barleyHordeum vulgaree1
- Organ / stage
- thylakoid membranee0
- Gene
- LHC Ib polypeptidee0
- Outcome
- temporally lags behind transcript accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LHC Ib polypeptide accumulation in the thylakoid membrane temporally lags behind transcript accumulation”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
LHC Ib transcripts accumulated more slowly than the major LHC IIb component.
- Taxon
- barleyHordeum vulgaree1
- Gene
- LHC Ib transcriptse0
- Outcome
- rates of accumulatione0
- Direction
- lag behinde0
- Comparator
- LHC IIbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rates of accumulation of LHC Ib transcripts and of their apoproteins lag behind those of the major LHC II component, LHC IIb”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
LHC Ib apoproteins accumulated more slowly than LHC IIb.
- Taxon
- barleyHordeum vulgaree1
- Gene
- apoproteinse0
- Outcome
- rates of accumulatione0
- Direction
- lag behinde0
- Comparator
- LHC IIbe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The rates of accumulation of LHC Ib transcripts and of their apoproteins lag behind those of the major LHC II component, LHC IIb”
e1“barley (Hordeum vulgare)”
Claim · model-written summary
Complete LHC Ib pigment-protein assembly required at least 72 hours of light exposure.
- Taxon
- barleyHordeum vulgaree1
- Organ / stage
- seedlingse0
- Treatment
- lighte0
- Gene
- LHC Ib pigment-proteine0
- Outcome
- Complete assemblye0
- Value
- 72 h or moree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complete assembly of the LHC Ib pigment-protein, as observed by low-temperature fluorescence spectroscopy, requires exposure of dark-grown seedlings to 72 h or more of light”
e1“barley (Hordeum vulgare)”
The same questions in other papers
light · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
18 papers in the same direction as this paper · 11 in the opposite direction
- negative
- no effect
- negative
26 papers more in this group
See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.