Abstract
Changes in mitochondrial respiration were investigated during cold storage of apple fruit ( Malus domestica Borkh. cv. Reinette du Canada). Postharvest calcium treatment of the apples had no effect on the aspects of mitochondrial metabolism studied, suggesting that the role of calcium in fruit ripening is not related to respiration. A transient increase in mitochondrial oxidative capacity occurred during storage around day 80 after harvest. This climacteric burst was associated with an enhanced capacity of the alternative pathway, but not of the cytochrome pathway. The major factor determining this rise in alternative pathway capacity at the climacteric was the transient increase in the amount of alternative oxidase (AOX) protein. Antibodies raised against the Sauromatum guttatum enzyme recognized a reduced form product of ampproximately 37 kDa and a 67‐kDa band corresponding to the oxidized form of the protein. During cold storage of the apple fruit, alternative pathway capacity correlated with the levels of the AOX and was ampparently not dependent on major changes in the oxidation state of the enzyme. This is the first work to report that the respiratory climacteric and the induction of AOX are tightly and temporally linked.
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Claims 10
Claim · model-written summary
Postharvest calcium had no effect on studied mitochondrial metabolism.
- Taxon
- apples
Malus domesticae0 - Treatment
- Postharvest calcium treatmente0
- Outcome
- mitochondrial metabolisme0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Postharvest calcium treatment of the apples had no effect on the aspects of mitochondrial metabolism studied”
Claim · model-written summary
Mitochondrial oxidative capacity rose around day 80.
- Taxon
- apple fruitMalus domesticat0
- Outcome
- mitochondrial oxidative capacitye0
- Direction
- transient increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A transient increase in mitochondrial oxidative capacity occurred during storage around day 80 after harvest”
t0“apple fruit. II. Alternative oxidase is induced”
Claim · model-written summary
Climacteric burst was associated with enhanced alternative pathway capacity.
- Taxon
- apple fruitMalus domesticat0
- Organ / stage
- fruitt0
- Outcome
- capacity of the alternative pathwaye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This climacteric burst was associated with an enhanced capacity of the alternative pathway”
t0“apple fruit. II. Alternative oxidase is induced”
Claim · model-written summary
Climacteric burst was not associated with enhanced cytochrome pathway capacity.
- Taxon
- apple fruitMalus domesticat0
- Organ / stage
- fruitt0
- Outcome
- cytochrome pathwaye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This climacteric burst was associated with an enhanced capacity of the alternative pathway, but not of the cytochrome pathway”
t0“apple fruit. II. Alternative oxidase is induced”
Claim · model-written summary
AOX protein increase chiefly determined the alternative pathway increase.
- Taxon
- apple fruitMalus domesticat0
- Gene
- alternative oxidase (AOX) proteine0
- Outcome
- alternative pathway capacitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The major factor determining this rise in alternative pathway capacity at the climacteric was the transient increase in the amount of alternative oxidase (AOX) protein”
t0“apple fruit. II. Alternative oxidase is induced”
Claim · model-written summary
Antibodies recognized a reduced product of approximately 37 kDa.
- Taxon
- apple fruitMalus domesticat0
- Gene
- Sauromatum guttatum enzymee0
- Outcome
- reduced form producte0
- Value
- 37 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Antibodies raised against the Sauromatum guttatum enzyme recognized a reduced form product of ampproximately 37 kDa”
t0“apple fruit. II. Alternative oxidase is induced”
Claim · model-written summary
Antibodies recognized an oxidized band of 67 kDa.
- Taxon
- apple fruitMalus domesticat0
- Outcome
- oxidized form of the proteine0
- Value
- 67‐kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 67‐kDa band corresponding to the oxidized form of the protein”
t0“apple fruit. II. Alternative oxidase is induced”
Claim · model-written summary
Alternative pathway capacity correlated with AOX levels.
- Taxon
- apple fruitMalus domesticae0
- Gene
- AOXe0
- Outcome
- alternative pathway capacitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During cold storage of the apple fruit, alternative pathway capacity correlated with the levels of the AOX”
Claim · model-written summary
Alternative pathway capacity apparently did not depend on AOX oxidation state.
- Taxon
- apple fruitMalus domesticae0
- Gene
- AOXe0
- Outcome
- alternative pathway capacitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During cold storage of the apple fruit, alternative pathway capacity correlated with the levels of the AOX and was ampparently not dependent on major changes in the oxidation state of the enzyme”
Claim · model-written summary
Respiratory climacteric and AOX induction were temporally linked.
- Taxon
- apple fruitMalus domesticat0
- Gene
- AOXe0
- Outcome
- respiratory climacterice0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the respiratory climacteric and the induction of AOX are tightly and temporally linked”
t0“apple fruit. II. Alternative oxidase is induced”
The same questions in other papers
calcium atom · molecular: metabolites · Malus domestica (apple)Results differ
3 papers in the same direction as this paper · 15 in the opposite direction
- positive
- positive
- positive
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.