Abstract
Oomycete pathogens cause serious damage to a wide spectrum of plants. Although host pathogen recognition via pathogen effectors and cognate plant resistance proteins is well established, the genetic basis of host factors that mediate plant susceptibility to oomycete pathogens is relatively unexplored. Here, we report on RTP1, a nodulin-related MtN21 family gene in Arabidopsis that mediates susceptibility to Phytophthora parasitica. RTP1 was identified by screening a T-DNA insertion mutant population and encoded an endoplasmic reticulum (ER)-localized protein. Overexpression of RTP1 rendered Arabidopsis more susceptible, whereas RNA silencing of RTP1 led to enhanced resistance to P. parasitica. Moreover, an RTP1 mutant, rtp1-1, displayed localized cell death, increased reactive oxygen species (ROS) production and accelerated PR1 expression, compared to the wild-type Col-0, in response to P. parasitica infection. rtp1-1 showed a similar disease response to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000, including increased disease resistance, cell death and ROS production. Furthermore, rpt1-1 exhibited resistance to the fungal pathogen Golovinomyces cichoracearum, but not to the necrotrophic pathogen Botrytis cinerea. Taken together, these results suggest that RTP1 negatively regulates plant resistance to biotrophic pathogens, possibly by regulating ROS production, cell death progression and PR1 expression.
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Claims 10
Claim · model-written summary
RTP1 encodes an endoplasmic-reticulum-localized protein.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- RTP1e0
- Outcome
- endoplasmic reticulum (ER)-localized proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RTP1 was identified by screening a T-DNA insertion mutant population and encoded an endoplasmic reticulum (ER)-localized protein”
t0“Arabidopsis”
Claim · model-written summary
RTP1 overexpression increased Arabidopsis susceptibility to Phytophthora parasitica.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of RTP1e0
- Interactor
- P. parasitica
Phytophthora nicotianaee0pathogen - Gene
- RTP1e0
- Outcome
- susceptiblee0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of RTP1 rendered Arabidopsis more susceptible, whereas RNA silencing of RTP1 led to enhanced resistance to P. parasitica”
Claim · model-written summary
RTP1 silencing enhanced Arabidopsis resistance to P. parasitica.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- RNA silencing of RTP1e0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Gene
- RTP1e0
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA silencing of RTP1 led to enhanced resistance to P. parasitica”
t0“Arabidopsis”
Claim · model-written summary
The rtp1-1 mutant showed localized cell death after P. parasitica infection relative to Col-0.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rtp1-1e0
- Treatment
- P. parasitica infectione0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Gene
- RTP1e0
- Outcome
- localized cell deathe0
- Comparator
- wild-type Col-0e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an RTP1 mutant, rtp1-1, displayed localized cell death, increased reactive oxygen species (ROS) production and accelerated PR1 expression, compared to the wild-type Col-0, in response to P. parasitica infection”
t0“Arabidopsis”
Claim · model-written summary
The rtp1-1 mutant increased ROS production after P. parasitica infection relative to Col-0.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rtp1-1e0
- Treatment
- P. parasitica infectione0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Outcome
- reactive oxygen species (ROS) productione0
- Direction
- increasede0
- Comparator
- wild-type Col-0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an RTP1 mutant, rtp1-1, displayed localized cell death, increased reactive oxygen species (ROS) production and accelerated PR1 expression, compared to the wild-type Col-0, in response to P. parasitica infection”
t0“Arabidopsis”
Claim · model-written summary
The rtp1-1 mutant accelerated PR1 expression after P. parasitica infection relative to Col-0.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rtp1-1e0
- Treatment
- P. parasitica infectione0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Gene
- PR1e0
- Outcome
- PR1 expressione0
- Direction
- acceleratede0
- Comparator
- wild-type Col-0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an RTP1 mutant, rtp1-1, displayed localized cell death, increased reactive oxygen species (ROS) production and accelerated PR1 expression, compared to the wild-type Col-0, in response to P. parasitica infection”
t0“Arabidopsis”
Claim · model-written summary
The rtp1-1 mutant had increased resistance, cell death and ROS production in response to Pseudomonas syringae pv. tomato DC3000.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rtp1-1e0
- Interactor
- Pseudomonas syringae pv. tomato (Pst) DC3000
Pseudomonas syringae pv. tomatoe0pathogen - Outcome
- disease resistance, cell death and ROS productione0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rtp1-1 showed a similar disease response to the bacterial pathogen Pseudomonas syringae pv. tomato (Pst) DC3000, including increased disease resistance, cell death and ROS production”
t0“Arabidopsis”
Claim · model-written summary
The rtp1-1 mutant resisted Golovinomyces cichoracearum.
- Taxon
- ArabidopsisArabidopsist0
- Material
- rpt1-1e0
- Interactor
- Golovinomyces cichoracearum
Golovinomyces cichoracearume0pathogen - Outcome
- resistancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rpt1-1 exhibited resistance to the fungal pathogen Golovinomyces cichoracearum”
t0“Arabidopsis”
Claim · model-written summary
The rtp1-1 mutant did not resist Botrytis cinerea.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- not to the necrotrophic pathogen Botrytis cinereae0
Not reportedMaterialDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not to the necrotrophic pathogen Botrytis cinerea”
t0“RTP1 encodes a novel endoplasmic reticulum (ER)‐localized protein in Arabidopsis and negatively regulates resistance against biotrophic pathogens”
Claim · model-written summary
The authors infer that RTP1 negatively regulates resistance to biotrophic pathogens, possibly through ROS, cell death, and PR1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RTP1e0
- Outcome
- plant resistance to biotrophic pathogense0
- Qualifier
- possibly by regulating ROS production, cell death progression and PR1 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RTP1 negatively regulates plant resistance to biotrophic pathogens, possibly by regulating ROS production, cell death progression and PR1 expression”
t0“Arabidopsis”
The same questions in other papers
loss of function · Phytophthora nicotianae · ArabidopsisPositive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.