Abstract
Oomycetes, particularly Phytophthora species, cause destructive plant diseases that severely threaten sustainable crop production. Due to the loss of genotype-specific disease resistance, it is important to identify and understand immune factors that mediate plant susceptibility. Loss-of-function of the susceptibility factor resistance to Phytophthora parasitica 1 (RTP1) leads to broad-spectrum disease resistance in Arabidopsis thaliana (A. thaliana (L.) Heynh.). Through RNA-seq analysis, we determined that CYP71B3, encoding an uncharacterized P450 enzyme, is significantly upregulated in rtp1 mutant plants infected with P. parasitica. Loss-of-function of CYP71B3 led to abolished pathogen-associated molecular pattern (PAMP)-triggered oxidative burst and rendered Arabidopsis more susceptible to diverse pathogens, including the oomycete P. parasitica and bacterial Pseudomonas syringae. Conversely, overexpression of CYP71B3 enhanced plant resistance and PAMP-triggered oxidative burst. CYP71B3 localized in the endoplasmic reticulum and was destabilized by interacting with RTP1 via the I-38 residue, which is essential for its immune function and P450 enzyme activity. The expression of CYP71B3 was regulated by transcription factor bZIP60, which is required for rtp1-mediated resistance to P. parasitica. Our studies indicate that RTP1 mediates plant susceptibility by destabilizing the downstream positive immune factor CYP71B3.
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Claims 9
Claim · model-written summary
RTP1 loss of function led to broad-spectrum disease resistance.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Loss-of-functione0
- Gene
- RTP1e0
- Outcome
- broad-spectrum disease resistancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of the susceptibility factor resistance to Phytophthora parasitica 1 (RTP1) leads to broad-spectrum disease resistance in Arabidopsis thaliana (A. thaliana (L.) Heynh.)”
Claim · model-written summary
CYP71B3 was significantly upregulated in infected rtp1 plants.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- P. parasitica
Phytophthora nicotianaee0pathogen - Gene
- CYP71B3e0
- Outcome
- CYP71B3e0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP71B3, encoding an uncharacterized P450 enzyme, is significantly upregulated in rtp1 mutant plants infected with P. parasitica”
t0“Arabidopsis”
Claim · model-written summary
CYP71B3 loss abolished the PAMP-triggered oxidative burst.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Loss-of-function of CYP71B3e0
- Gene
- CYP71B3e0
- Outcome
- pathogen-associated molecular pattern (PAMP)-triggered oxidative burste0
- Direction
- abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of CYP71B3 led to abolished pathogen-associated molecular pattern (PAMP)-triggered oxidative burst and rendered Arabidopsis more susceptible to diverse pathogens, including the oomycete P. parasitica and bacterial Pseudomonas syringae”
Claim · model-written summary
CYP71B3 loss increased susceptibility to P. parasitica.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Loss-of-function of CYP71B3e0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Outcome
- susceptiblee0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of CYP71B3 led to abolished pathogen-associated molecular pattern (PAMP)-triggered oxidative burst and rendered Arabidopsis more susceptible to diverse pathogens, including the oomycete P. parasitica and bacterial Pseudomonas syringae”
Claim · model-written summary
CYP71B3 loss increased susceptibility to Pseudomonas syringae.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Loss-of-function of CYP71B3e0
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee0pathogen - Outcome
- susceptiblee0
- Direction
- moree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Loss-of-function of CYP71B3 led to abolished pathogen-associated molecular pattern (PAMP)-triggered oxidative burst and rendered Arabidopsis more susceptible to diverse pathogens, including the oomycete P. parasitica and bacterial Pseudomonas syringae”
Claim · model-written summary
CYP71B3 overexpression enhanced plant resistance and oxidative burst.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpression of CYP71B3e0
- Gene
- CYP71B3e0
- Outcome
- plant resistance and PAMP-triggered oxidative burste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of CYP71B3 enhanced plant resistance and PAMP-triggered oxidative burst”
t0“Arabidopsis”
Claim · model-written summary
CYP71B3 localized in the endoplasmic reticulum.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CYP71B3e0
- Outcome
- localized in the endoplasmic reticulume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP71B3 localized in the endoplasmic reticulum”
t0“Arabidopsis”
Claim · model-written summary
RTP1 interaction destabilized CYP71B3 via I-38.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RTP1e0
- Outcome
- CYP71B3e0
- Direction
- destabilizede0
- Qualifier
- I-38 residuee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CYP71B3 localized in the endoplasmic reticulum and was destabilized by interacting with RTP1 via the I-38 residue”
t0“Arabidopsis”
Claim · model-written summary
bZIP60 regulated CYP71B3 expression and was required for rtp1-mediated resistance.
- Taxon
- ArabidopsisArabidopsist0
- Interactor
- P. parasiticaPhytophthora nicotianaee0pathogen
- Gene
- bZIP60e0
- Outcome
- expression of CYP71B3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of CYP71B3 was regulated by transcription factor bZIP60, which is required for rtp1-mediated resistance to P. parasitica”
t0“Arabidopsis”
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