Abstract
Previous studies have demonstrated that methyl jasmonate (MeJA) induces stomatal closure dependent on change of cytosolic free calcium concentration in guard cells. However, these molecular mechanisms of intracellular Ca(2+) signal perception remain unknown. Calcium-dependent protein kinases (CDPKs) function as Ca(2+) signal transducers in various plant physiological processes. It has been reported that four Arabidopsis (Arabidopsis thaliana) CDPKs, CPK3, CPK6, CPK4, and CPK11, are involved in abscisic acid signaling in guard cells. It is also known that there is an interaction between MeJA and abscisic acid signaling in guard cells. In this study, we examined the roles of these CDPKs in MeJA signaling in guard cells using Arabidopsis mutants disrupted in the CDPK genes. Disruption of the CPK6 gene impaired MeJA-induced stomatal closure, but disruption of the other CDPK genes did not. Despite the broad expression pattern of CPK6, we did not find other remarkable MeJA-insensitive phenotypes in the cpk6-1 mutant. The whole-cell patch-clamp analysis revealed that MeJA activation of nonselective Ca(2+)-permeable cation channels is impaired in the cpk6-1 mutant. Consistent with this result, MeJA-induced transient cytosolic free calcium concentration increments were reduced in the cpk6-1 mutant. MeJA failed to activate slow-type anion channels in the cpk6-1 guard cells. Production of early signal components, reactive oxygen species and nitric oxide, in guard cells was elicited by MeJA in the cpk6-1 mutant as in the wild type. These results provide genetic evidence that CPK6 has a different role from CPK3 and functions as a positive regulator of MeJA signaling in Arabidopsis guard cells.
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Claims 8
Claim · model-written summary
CPK6 disruption impaired methyl jasmonate induced stomatal closure.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- Disruption of the CPK6 genee0
- Gene
- CPK6e0
- Outcome
- MeJA-induced stomatal closuree0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of the CPK6 gene impaired MeJA-induced stomatal closure”
t0“Arabidopsis”
Claim · model-written summary
Disruption of the other CDPK genes did not impair methyl jasmonate induced closure.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- disruption of the other CDPK genese0
- Gene
- other CDPK genese0
- Outcome
- MeJA-induced stomatal closuree0
- Direction
- did note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of the CPK6 gene impaired MeJA-induced stomatal closure, but disruption of the other CDPK genes did not”
t0“Arabidopsis”
Claim · model-written summary
No other marked methyl jasmonate insensitive phenotype was found in cpk6-1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cpk6-1 mutante0
- Outcome
- other remarkable MeJA-insensitive phenotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we did not find other remarkable MeJA-insensitive phenotypes in the cpk6-1 mutant”
t0“Arabidopsis”
Claim · model-written summary
Methyl jasmonate activation of calcium permeable cation channels was impaired in cpk6-1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cpk6-1 mutante0
- Outcome
- MeJA activation of nonselective Ca(2+)-permeable cation channelse0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA activation of nonselective Ca(2+)-permeable cation channels is impaired in the cpk6-1 mutant”
t0“Arabidopsis”
Claim · model-written summary
Methyl jasmonate induced cytosolic calcium increments were reduced in cpk6-1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cpk6-1 mutante0
- Outcome
- cytosolic free calcium concentration incrementse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA-induced transient cytosolic free calcium concentration increments were reduced in the cpk6-1 mutant”
t0“Arabidopsis”
Claim · model-written summary
Methyl jasmonate failed to activate slow anion channels in cpk6-1 guard cells.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cpk6-1e0
- Organ / stage
- guard cellse0
- Outcome
- slow-type anion channelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MeJA failed to activate slow-type anion channels in the cpk6-1 guard cells”
t0“Arabidopsis”
Claim · model-written summary
Methyl jasmonate elicited reactive oxygen species and nitric oxide in cpk6-1 as in wild type.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cpk6-1 mutante0
- Organ / stage
- guard cellse0
- Outcome
- reactive oxygen species and nitric oxidee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reactive oxygen species and nitric oxide, in guard cells was elicited by MeJA in the cpk6-1 mutant as in the wild type”
t0“Arabidopsis”
Claim · model-written summary
CPK6 functions as a positive regulator of methyl jasmonate signaling in guard cells.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- guard cellse0
- Gene
- CPK6e0
- Outcome
- MeJA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CPK6 has a different role from CPK3 and functions as a positive regulator of MeJA signaling in Arabidopsis guard cells”
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