Abstract
Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves. The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase. Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR). The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09 : 00 and 12 : 00, respectively. Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves, while the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth. These results suggest that the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation.
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Claims 8
Claim · model-written summary
A cDNA encoding cucumber CPD photolyase was isolated from leaves.
- Taxon
- cucumber
Cucumis sativuse0 - Organ / stage
- leavese0
- Gene
- CPD photolyasee0
- Outcome
- Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Complementary DNA encoding a putative cyclobutane pyrimidine dimer (CPD)-specific DNA photolyase (CPD photolyase) was isolated from cucumber leaves.”
Claim · model-written summary
The cucumber cDNA protein sequence was highly similar to Arabidopsis CPD photolyase.
- Taxon
- CucumberCucumis sativust0
- Gene
- Arabidopsis CPD photolyasee0
- Outcome
- high similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The deduced amino acid sequence of the cDNA exhibited high similarity to that of the Arabidopsis CPD photolyase.”
t0“Cucumber”
Claim · model-written summary
The cDNA restored photorepair in an E. coli mutant, supporting functional cucumber photolyase identity.
- Taxon
- cucumberCucumis sativuse0
- Treatment
- Transformation with the cDNAe0
- Gene
- cucumber CPD photolyase (CsPHR)e0
- Outcome
- photorepair activitye0
- Direction
- restorede0
- Comparator
- impaired photorepair activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transformation with the cDNA restored the impaired photorepair activity of an Escherichia coli mutant, indicating that this cDNA encodes a functional cucumber CPD photolyase (CsPHR).”
Claim · model-written summary
CsPHR transcripts peaked at 09 : 00 in cucumber first leaves.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- first leavese0
- Gene
- CsPHRe0
- Outcome
- level of CsPHR transcriptse0
- Direction
- peakinge0
- Value
- 09 : 00e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09 : 00 and 12 : 00, respectively.”
Claim · model-written summary
CPD photorepair activity peaked at 12 : 00 in cucumber first leaves.
- Taxon
- cucumberCucumis sativuse0
- Organ / stage
- first leavese0
- Outcome
- CPD photorepair activitye0
- Direction
- peakinge0
- Value
- 12 : 00e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of CsPHR transcripts estimated by quantitative RT-PCR as well as the CPD photorepair activity in the cucumber first leaves showed diurnal changes, peaking at 09 : 00 and 12 : 00, respectively.”
Claim · model-written summary
Midday supplemental UV-B had little effect on first leaf growth.
- Taxon
- CucumberCucumis sativust0
- Organ / stage
- first leavese0
- Treatment
- Supplemental UV-B irradiation in the middle of the light periode0
- Outcome
- growth of the first leavese0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supplemental UV-B irradiation in the middle of the light period had little effect on the growth of the first leaves,”
t0“Cucumber”
Claim · model-written summary
Early morning or late afternoon supplemental UV-B strongly retarded first leaf growth.
- Taxon
- CucumberCucumis sativust0
- Organ / stage
- leafe0
- Treatment
- supplemental irradiation in the early morning or late afternoone0
- Outcome
- leaf growthe0
- Direction
- retardede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the supplemental irradiation in the early morning or late afternoon strongly retarded the leaf growth.”
t0“Cucumber”
Claim · model-written summary
Authors suggest diurnal CPD repair activity, possibly regulated by CsPHR transcripts, minimizes UV-B growth inhibition.
- Taxon
- CucumberCucumis sativust0
- Gene
- CsPHRe0
- Outcome
- growth inhibition due to UV-B irradiatione0
- Qualifier
- diurnal change in CPD photorepair activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the diurnal change in CPD photorepair activity, which is presumably regulated by the transcript level of CsPHR, may play an important role in minimizing the growth inhibition due to UV-B irradiation.”
t0“Cucumber”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.