Abstract
In flowering plants, pollen germinates on the stigma and pollen tubes grow through the style to fertilize the ovules. Enzymatic production of reactive oxygen species (ROS) has been suggested to be involved in pollen tube tip growth. Here, we characterized the function and regulation of the NADPH oxidases RbohH and RbohJ (Respiratory burst oxidase homolog H and J) in pollen tubes in Arabidopsis thaliana. In the rbohH and rbohJ single mutants, pollen tube tip growth was comparable to that of the wild type; however, tip growth was severely impaired in the double mutant. In vivo imaging showed that ROS accumulation in the pollen tube was impaired in the double mutant. Both RbohH and RbohJ, which contain Ca(2+) binding EF-hand motifs, possessed Ca(2+)-induced ROS-producing activity and localized at the plasma membrane of the pollen tube tip. Point mutations in the EF-hand motifs impaired Ca(2+)-induced ROS production and complementation of the double mutant phenotype. We also showed that a protein phosphatase inhibitor enhanced the Ca(2+)-induced ROS-producing activity of RbohH and RbohJ, suggesting their synergistic activation by protein phosphorylation and Ca(2+). Our results suggest that ROS production by RbohH and RbohJ is essential for proper pollen tube tip growth, and furthermore, that Ca(2+)-induced ROS positive feedback regulation is conserved in the polarized cell growth to shape the long tubular cell.
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Claims 10
Claim · model-written summary
Single mutants had pollen tube tip growth comparable to wild type.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Material
- rbohH and rbohJ single mutantse0
- Outcome
- pollen tube tip growthe0
- Direction
- comparablee0
- Comparator
- wild typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the rbohH and rbohJ single mutants, pollen tube tip growth was comparable to that of the wild type”
t0“Arabidopsis thaliana”
Claim · model-written summary
The double mutant had severely impaired pollen tube tip growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- double mutante0
- Outcome
- tip growthe0
- Direction
- severely impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tip growth was severely impaired in the double mutant.”
t0“Arabidopsis thaliana”
Claim · model-written summary
ROS accumulation in the pollen tube was impaired in the double mutant.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- double mutante0
- Outcome
- ROS accumulation in the pollen tubee0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS accumulation in the pollen tube was impaired in the double mutant.”
t0“Arabidopsis thaliana”
Claim · model-written summary
RbohH and RbohJ had calcium-induced ROS-producing activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RbohH and RbohJe0
- Outcome
- Ca(2+)-induced ROS-producing activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both RbohH and RbohJ, which contain Ca(2+) binding EF-hand motifs, possessed Ca(2+)-induced ROS-producing activity and localized at the plasma membrane of the pollen tube tip.”
t0“Arabidopsis thaliana”
Claim · model-written summary
RbohH and RbohJ localized at the pollen tube tip plasma membrane.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RbohH and RbohJe0
- Outcome
- localized at the plasma membrane of the pollen tube tipe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both RbohH and RbohJ, which contain Ca(2+) binding EF-hand motifs, possessed Ca(2+)-induced ROS-producing activity and localized at the plasma membrane of the pollen tube tip.”
t0“Arabidopsis thaliana”
Claim · model-written summary
EF-hand point mutations impaired calcium-induced ROS production.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Point mutations in the EF-hand motifse0
- Outcome
- Ca(2+)-induced ROS productione0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Point mutations in the EF-hand motifs impaired Ca(2+)-induced ROS production and complementation of the double mutant phenotype.”
t0“Arabidopsis thaliana”
Claim · model-written summary
EF-hand point mutations impaired complementation of the double mutant phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Point mutations in the EF-hand motifse0
- Outcome
- complementation of the double mutant phenotypee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Point mutations in the EF-hand motifs impaired Ca(2+)-induced ROS production and complementation of the double mutant phenotype.”
t0“Arabidopsis thaliana”
Claim · model-written summary
A protein phosphatase inhibitor enhanced calcium-induced ROS production by RbohH and RbohJ.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- a protein phosphatase inhibitore0
- Gene
- RbohH and RbohJe0
- Outcome
- Ca(2+)-induced ROS-producing activitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a protein phosphatase inhibitor enhanced the Ca(2+)-induced ROS-producing activity of RbohH and RbohJ”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors infer that ROS production by RbohH and RbohJ is essential for proper pollen tube tip growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- RbohH and RbohJe0
- Outcome
- proper pollen tube tip growthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS production by RbohH and RbohJ is essential for proper pollen tube tip growth”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors infer conserved calcium-induced ROS positive feedback during polarized cell growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- polarized cell growthe0
- Qualifier
- Ca(2+)-induced ROS positive feedback regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ca(2+)-induced ROS positive feedback regulation is conserved in the polarized cell growth to shape the long tubular cell.”
t0“Arabidopsis thaliana”
The same questions in other papers
loss of function · physiology: oxidative · Arabidopsis thaliana (thale cress)Results agree
4 papers in the same direction as this paper
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.