Abstract
Aluminum (Al) stress triggers the accumulation of hydrogen peroxide (H2O2) in roots. However, whether H2O2 plays a regulatory role in aluminum resistance remains unclear. In this study, we show that H2O2 plays a crucial role in regulation of Al resistance, which is modulated by the mitochondrion-localized pentatricopeptide repeat protein REGULATION OF ALMT1 EXPRESSION 6 (RAE6). Mutation in RAE6 impairs the activity of complex I of the mitochondrial electron transport chain, resulting in the accumulation of H2O2 and increased sensitivity to Al. Our results suggest that higher H2O2 concentrations promote the oxidation of SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1), an essential transcription factor that promotes Al resistance, thereby promoting its degradation by enhancing the interaction between STOP1 and the F-box protein RAE1. Conversely, decreasing H2O2 levels or blocking the oxidation of STOP1 leads to greater STOP1 stability and increased Al resistance. Moreover, we show that the thioredoxin TRX1 interacts with STOP1 to catalyze its chemical reduction. Thus, our results highlight the importance of H2O2 in Al resistance and regulation of STOP1 stability in Arabidopsis (Arabidopsis thaliana).
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
RAE6 mutation impaired mitochondrial complex I activity.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianat0 - Treatment
- Mutation in RAE6e0
- Gene
- RAE6e0
- Outcome
- activity of complex Ie0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in RAE6 impairs the activity of complex I of the mitochondrial electron transport chain”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
RAE6 mutation increased H2O2 accumulation.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Treatment
- Mutation in RAE6e0
- Gene
- RAE6e0
- Outcome
- accumulation of H2O2e0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in RAE6 impairs the activity of complex I of the mitochondrial electron transport chain, resulting in the accumulation of H2O2”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
RAE6 mutation increased aluminum sensitivity.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Treatment
- Mutation in RAE6e0
- Gene
- RAE6e0
- Outcome
- sensitivity to Ale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation in RAE6 impairs the activity of complex I of the mitochondrial electron transport chain, resulting in the accumulation of H2O2 and increased sensitivity to Al”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Higher H2O2 promoted STOP1 oxidation.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Gene
- SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1)e0
- Compound
- H2O2e0
- Outcome
- oxidation of SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher H2O2 concentrations promote the oxidation of SENSITIVE TO PROTON RHIZOTOXICITY 1 (STOP1)”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
STOP1 oxidation promoted degradation through stronger STOP1–RAE1 interaction.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Gene
- STOP1e0
- Outcome
- degradatione0
- Qualifier
- enhancing the interaction between STOP1 and the F-box protein RAE1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoting its degradation by enhancing the interaction between STOP1 and the F-box protein RAE1”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Decreasing H2O2 increased STOP1 stability and aluminum resistance.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Treatment
- decreasing H2O2 levelse0
- Gene
- STOP1e0
- Outcome
- STOP1 stability and increased Al resistancee0
- Direction
- greatere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreasing H2O2 levels or blocking the oxidation of STOP1 leads to greater STOP1 stability and increased Al resistance”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Blocking STOP1 oxidation increased STOP1 stability and aluminum resistance.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Treatment
- blocking the oxidation of STOP1e0
- Gene
- STOP1e0
- Outcome
- STOP1 stability and increased Al resistancee0
- Direction
- greatere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“decreasing H2O2 levels or blocking the oxidation of STOP1 leads to greater STOP1 stability and increased Al resistance”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
TRX1 interacted with STOP1 and catalyzed its chemical reduction.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianat0
- Gene
- thioredoxin TRX1e0
- Outcome
- chemical reductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thioredoxin TRX1 interacts with STOP1 to catalyze its chemical reduction”
t0“Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
hydrogen peroxide · disease · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
loss of function · nutrient: content · Arabidopsis thaliana (thale cress)Positive and negative results
18 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
22 papers more in this group
See all 26 papers →loss of function · physiology: oxidative · Arabidopsis thaliana (thale cress)Results agree
4 papers in the same direction as this paper
- no effect
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.