Abstract
The significance of plastid terminal oxidase (PTOX) in phytoene desaturation and chloroplast function has been demonstrated using PTOX-deficient mutants, particularly in Arabidopsis. However, studies on its role in monocots are lacking. Here, we report cloning and characterization of the rice (Oryza sativa) PTOX1 gene. Using Ecotype Targeting Induced Local Lesions IN Genomes (EcoTILLING) and TILLING as forward genetic tools, we identified the causative mutation of an EMS mutant characterized by excessive tillering, semi-dwarfism and leaf variegation that corresponded to the PTOX1 gene. The tillering and semi-dwarf phenotypes of the ptox1 mutant are similar to phenotypes of known strigolactone (SL)-related rice mutants, and both phenotypic traits could be rescued by application of the synthetic SL GR24. The ptox1 mutant accumulated phytoene in white leaf sectors with a corresponding deficiency in β-carotene, consistent with the expected function of PTOX1 in promoting phytoene desaturase activity. There was also no accumulation of the carotenoid-derived SL ent-2'-epi-5-deoxystrigol in root exudates. Elevated concentrations of auxin were detected in the mutant, supporting previous observations that SL interaction with auxin is important in shoot branching control. Our results demonstrate that PTOX1 is required for both carotenoid and SL synthesis resulting in SL-deficient phenotypes in rice.
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Claims 9
Claim · model-written summary
The causative mutation of a rice mutant with excessive tillering, semi-dwarfism and leaf variegation corresponded to PTOX1.
- Taxon
- Oryza sativa
Oryza sativae1 - Material
- EMS mutante0
- Gene
- PTOX1e0
- Outcome
- causative mutatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified the causative mutation of an EMS mutant characterized by excessive tillering, semi-dwarfism and leaf variegation that corresponded to the PTOX1 gene”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
The ptox1 mutant had tillering and semi-dwarf phenotypes similar to strigolactone-related rice mutants.
- Taxon
- Oryza sativaOryza sativae1
- Material
- ptox1 mutante0
- Outcome
- tillering and semi-dwarf phenotypese0
- Comparator
- known strigolactone (SL)-related rice mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tillering and semi-dwarf phenotypes of the ptox1 mutant are similar to phenotypes of known strigolactone (SL)-related rice mutants”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
Synthetic strigolactone GR24 rescued the ptox1 tillering phenotype.
- Taxon
- Oryza sativaOryza sativae1
- Material
- ptox1 mutante0
- Treatment
- application of the synthetic SL GR24e0
- Outcome
- tilleringe0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tillering and semi-dwarf phenotypes of the ptox1 mutant are similar to phenotypes of known strigolactone (SL)-related rice mutants, and both phenotypic traits could be rescued by application of the synthetic SL GR24”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
Synthetic strigolactone GR24 rescued the ptox1 semi-dwarf phenotype.
- Taxon
- Oryza sativaOryza sativae1
- Material
- ptox1 mutante0
- Treatment
- application of the synthetic SL GR24e0
- Outcome
- semi-dwarf phenotypese0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tillering and semi-dwarf phenotypes of the ptox1 mutant are similar to phenotypes of known strigolactone (SL)-related rice mutants, and both phenotypic traits could be rescued by application of the synthetic SL GR24”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
The ptox1 mutant accumulated phytoene in white leaf sectors.
- Taxon
- Oryza sativaOryza sativae1
- Material
- ptox1 mutante0
- Organ / stage
- white leaf sectorse0
- Outcome
- phytoenee0
- Direction
- accumulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ptox1 mutant accumulated phytoene in white leaf sectors”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
The ptox1 mutant was deficient in β-carotene in white leaf sectors.
- Taxon
- Oryza sativaOryza sativae1
- Material
- ptox1 mutante0
- Organ / stage
- white leaf sectorse0
- Outcome
- β-carotenee0
- Direction
- deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ptox1 mutant accumulated phytoene in white leaf sectors with a corresponding deficiency in β-carotene”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
The strigolactone ent-2'-epi-5-deoxystrigol did not accumulate in ptox1 root exudates.
- Taxon
- Oryza sativaOryza sativae1
- Organ / stage
- root exudatese0
- Outcome
- ent-2'-epi-5-deoxystrigole0
- Direction
- no accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was also no accumulation of the carotenoid-derived SL ent-2'-epi-5-deoxystrigol in root exudates”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
Auxin concentrations were elevated in the ptox1 mutant.
- Taxon
- Oryza sativaOryza sativae1
- Material
- mutante0
- Outcome
- concentrations of auxine0
- Direction
- Elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Elevated concentrations of auxin were detected in the mutant”
e1“rice (Oryza sativa) PTOX1 gene”
Claim · model-written summary
PTOX1 is required for carotenoid and strigolactone synthesis in rice.
- Taxon
- riceOryza sativae0
- Gene
- PTOX1e0
- Outcome
- carotenoid and SL synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PTOX1 is required for both carotenoid and SL synthesis resulting in SL-deficient phenotypes in rice”
The same questions in other papers
class · growth · Oryza sativa (rice)Positive and negative results
61 papers in the same direction as this paper · 17 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- no effect
- negative
76 papers more in this group
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