Abstract
The Salt Overly Sensitive (SOS) pathway plays an important role in the regulation of Na+/K+ ion homeostasis and salt tolerance in Arabidopsis thaliana. Previously, we reported that the calcium binding proteins SOS3 and SOS3-LIKE CALCIUM BINDING PROTEIN8 (SCaBP8) nonredundantly activate the protein kinase SOS2. Here, we show that SOS2 phosphorylates SCaBP8 at its C terminus but does not phosphorylate SOS3. In vitro, SOS2 phosphorylation of SCaBP8 was enhanced by the bimolecular interaction of SOS2 and SCaBP8 and did not require calcium ions. In vivo, this phosphorylation was induced by salt stress, occurred at the membrane, stabilized the SCaBP8-SOS2 interaction, and enhanced plasma membrane Na+/H+ exchange activity. When a Ser at position 237 in the SCaBP8 protein (the SOS2 phosphorylation target) was mutated to Ala, SCaBP8 was no longer phosphorylated by SOS2 and the mutant protein could not fully rescue the salt-sensitive phenotype of the scabp8 mutant. By contrast, when Ser-237 was mutated to Asp to mimic the charge of a phosphorylated Ser residue, the mutant protein rescued the scabp8 salt sensitivity. These data demonstrate that calcium sensor phosphorylation is a critical component of SOS pathway regulation of salt tolerance in Arabidopsis.
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Claims 10
Claim · model-written summary
SOS2 phosphorylated SCaBP8 at its C terminus.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Gene
- SOS2e0
- Outcome
- phosphorylates SCaBP8 at its C terminuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SOS2 phosphorylates SCaBP8 at its C terminus”
e1“Arabidopsis thaliana”
Claim · model-written summary
SOS2 did not phosphorylate SOS3.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SOS3e0
- Outcome
- does not phosphorylate SOS3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“does not phosphorylate SOS3”
e1“Arabidopsis thaliana”
Claim · model-written summary
In vitro SOS2-SCaBP8 interaction enhanced SCaBP8 phosphorylation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SOS2e0
- Outcome
- SOS2 phosphorylation of SCaBP8e0
- Direction
- enhancede0
- Qualifier
- bimolecular interaction of SOS2 and SCaBP8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro, SOS2 phosphorylation of SCaBP8 was enhanced by the bimolecular interaction of SOS2 and SCaBP8”
e1“Arabidopsis thaliana”
Claim · model-written summary
In vitro SCaBP8 phosphorylation did not require calcium ions.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Compound
- calcium ionse0
- Outcome
- SOS2 phosphorylation of SCaBP8e1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did not require calcium ions”
e1“SOS2 phosphorylation of SCaBP8”
e2“Arabidopsis thaliana”
Claim · model-written summary
SCaBP8 phosphorylation stabilized SCaBP8-SOS2 interaction.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SCaBP8-SOS2e0
- Outcome
- SCaBP8-SOS2 interactione0
- Direction
- stabilizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this phosphorylation was induced by salt stress, occurred at the membrane, stabilized the SCaBP8-SOS2 interaction”
e1“Arabidopsis thaliana”
Claim · model-written summary
SCaBP8 phosphorylation enhanced plasma membrane Na+/H+ exchange activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- SCaBP8-SOS2e0
- Outcome
- plasma membrane Na+/H+ exchange activitye0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this phosphorylation was induced by salt stress, occurred at the membrane, stabilized the SCaBP8-SOS2 interaction, and enhanced plasma membrane Na+/H+ exchange activity.”
e1“Arabidopsis thaliana”
Claim · model-written summary
Substituting Ala for Ser at SCaBP8 position 237 prevented phosphorylation by SOS2.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- mutated to Alae0
- Gene
- SCaBP8e0
- Outcome
- SCaBP8 was no longer phosphorylated by SOS2e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When a Ser at position 237 in the SCaBP8 protein (the SOS2 phosphorylation target) was mutated to Ala, SCaBP8 was no longer phosphorylated by SOS2”
e1“Arabidopsis thaliana”
Claim · model-written summary
The Ala mutant protein could not fully rescue scabp8 salt sensitivity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae2
- Material
- scabp8 mutante0
- Treatment
- mutated to Alae1
- Outcome
- salt-sensitive phenotypee0
- Direction
- could not fully rescuee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mutant protein could not fully rescue the salt-sensitive phenotype of the scabp8 mutant.”
e1“mutated to Ala”
e2“Arabidopsis thaliana”
Claim · model-written summary
Asp substitution at Ser-237 rescued scabp8 salt sensitivity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- scabp8e0
- Treatment
- Ser-237 was mutated to Aspe0
- Outcome
- scabp8 salt sensitivitye0
- Direction
- rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when Ser-237 was mutated to Asp to mimic the charge of a phosphorylated Ser residue, the mutant protein rescued the scabp8 salt sensitivity.”
e1“Arabidopsis thaliana”
Claim · model-written summary
The data indicate calcium sensor phosphorylation is critical for SOS pathway regulation of salt tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Outcome
- salt tolerancee0
- Qualifier
- calcium sensor phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“calcium sensor phosphorylation is a critical component of SOS pathway regulation of salt tolerance in Arabidopsis.”
e1“Arabidopsis thaliana”
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