Abstract
WRKY proteins belong to a transcription factor (TF) family and play dynamic roles in many plant processes, including plant responses to abiotic and biotic stresses, as well as secondary metabolism. However, no WRKY gene in Panax ginseng C.A. Meyer has been reported to date. In this study, a number of WRKY unigenes from methyl jasmonate (MeJA)-treated adventitious root transcriptome of this species were identified using next-generation sequencing technology. A total of 48 promising WRKY unigenes encoding WRKY proteins were obtained by eliminating wrong and incomplete open reading frame (ORF). Phylogenetic analysis reveals 48 WRKY TFs, including 11 Group I, 36 Group II, and 1 Group III. Moreover, one MeJA-responsive unigene designated as PgWRKY1 was cloned and characterized. It contains an entire ORF of 1077 bp and encodes a polypeptide of 358 amino acid residues. The PgWRKY1 protein contains a single WRKY domain consisting of a conserved amino acid sequence motif WRKYGQK and a C2H2-type zinc-finger motif belonging to WRKY subgroup II-d. Subcellular localization of PgWRKY1-GFP fusion protein in onion and tobacco epidermis cells revealed that PgWRKY1 was exclusively present in the nucleus. Quantitative real-time polymerase chain reaction analysis demonstrated that the expression of PgWRKY1 was relatively higher in roots and lateral roots compared with leaves, stems, and seeds. Importantly, PgWRKY1 expression was significantly induced by salicylic acid, abscisic acid, and NaCl, but downregulated by MeJA treatment. These results suggested that PgWRKY1 might be a multiple stress-inducible gene responding to hormones and salt stresses.
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Claims 12
Claim · model-written summary
Forty-eight WRKY unigenes encoding WRKY proteins were obtained from the MeJA-treated adventitious root transcriptome of Panax ginseng.
- Taxon
- Panax ginseng
Panax ginsenge1 - Outcome
- WRKY unigenes encoding WRKY proteinse0
- Value
- 48e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 48 promising WRKY unigenes encoding WRKY proteins were obtained”
e1“Panax ginseng”
Claim · model-written summary
Phylogenetic analysis placed 11 of the 48 ginseng WRKY TFs in Group I.
- Taxon
- Panax ginsengPanax ginsenge1
- Outcome
- 11 Group Ie0
- Value
- 11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis reveals 48 WRKY TFs, including 11 Group I, 36 Group II, and 1 Group III”
e1“Panax ginseng”
Claim · model-written summary
Phylogenetic analysis placed 36 of the 48 ginseng WRKY TFs in Group II.
- Taxon
- Panax ginsengPanax ginsenge1
- Outcome
- 36 Group IIe0
- Value
- 36e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis reveals 48 WRKY TFs, including 11 Group I, 36 Group II, and 1 Group III”
e1“Panax ginseng”
Claim · model-written summary
Phylogenetic analysis placed 1 of the 48 ginseng WRKY TFs in Group III.
- Taxon
- Panax ginsengPanax ginsenge1
- Outcome
- 1 Group IIIe0
- Value
- 1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis reveals 48 WRKY TFs, including 11 Group I, 36 Group II, and 1 Group III”
e1“Panax ginseng”
Claim · model-written summary
The PgWRKY1 unigene contains an entire ORF of 1077 bp.
- Taxon
- Panax ginsengPanax ginsenge1
- Gene
- PgWRKY1e0
- Outcome
- entire ORFe0
- Value
- 1077 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one MeJA-responsive unigene designated as PgWRKY1 was cloned and characterized. It contains an entire ORF of 1077 bp and encodes a polypeptide of 358 amino acid residues”
e1“Panax ginseng”
Claim · model-written summary
PgWRKY1 encodes a polypeptide of 358 amino acid residues.
- Taxon
- Panax ginsengPanax ginsenge1
- Gene
- PgWRKY1e0
- Outcome
- polypeptidee0
- Value
- 358 amino acid residuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“one MeJA-responsive unigene designated as PgWRKY1 was cloned and characterized. It contains an entire ORF of 1077 bp and encodes a polypeptide of 358 amino acid residues”
e1“Panax ginseng”
Claim · model-written summary
The PgWRKY1 protein contains a single WRKY domain with the conserved motif WRKYGQK and a C2H2-type zinc-finger motif belonging to WRKY subgroup II-d.
- Taxon
- Panax ginsengPanax ginsenge1
- Gene
- PgWRKY1 proteine0
- Outcome
- a single WRKY domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PgWRKY1 protein contains a single WRKY domain consisting of a conserved amino acid sequence motif WRKYGQK and a C2H2-type zinc-finger motif belonging to WRKY subgroup II-d”
e1“Panax ginseng”
Claim · model-written summary
PgWRKY1-GFP fusion protein expressed in onion and tobacco epidermis cells was exclusively present in the nucleus.
- Taxon
- onion and tobacco
Nicotiana tabacum
Allium cepae0 - Organ / stage
- epidermis cellse0
- Gene
- PgWRKY1e0
- Outcome
- exclusively present in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Subcellular localization of PgWRKY1-GFP fusion protein in onion and tobacco epidermis cells revealed that PgWRKY1 was exclusively present in the nucleus”
Claim · model-written summary
PgWRKY1 expression was relatively higher in roots and lateral roots than in leaves, stems and seeds.
- Taxon
- Panax ginsengPanax ginsenge1
- Organ / stage
- roots and lateral rootse0
- Gene
- PgWRKY1e0
- Outcome
- expression of PgWRKY1e0
- Direction
- highere0
- Comparator
- leaves, stems, and seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of PgWRKY1 was relatively higher in roots and lateral roots compared with leaves, stems, and seeds”
e1“Panax ginseng”
Claim · model-written summary
PgWRKY1 expression was significantly induced by salicylic acid, abscisic acid and NaCl.
- Taxon
- Panax ginsengPanax ginsenge1
- Treatment
- salicylic acid, abscisic acid, and NaCle0
- Gene
- PgWRKY1e0
- Outcome
- PgWRKY1 expressione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PgWRKY1 expression was significantly induced by salicylic acid, abscisic acid, and NaCl, but downregulated by MeJA treatment”
e1“Panax ginseng”
Claim · model-written summary
PgWRKY1 expression was downregulated by MeJA treatment.
- Taxon
- Panax ginsengPanax ginsenge1
- Treatment
- MeJA treatmente0
- Gene
- PgWRKY1e0
- Outcome
- PgWRKY1 expressione0
- Direction
- downregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PgWRKY1 expression was significantly induced by salicylic acid, abscisic acid, and NaCl, but downregulated by MeJA treatment”
e1“Panax ginseng”
Claim · model-written summary
The authors suggest PgWRKY1 might be a multiple stress-inducible gene responding to hormones and salt stresses.
- Taxon
- Panax ginsengPanax ginsenge1
- Gene
- PgWRKY1e0
- Outcome
- multiple stress-inducible genee0
- Qualifier
- responding to hormones and salt stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggested that PgWRKY1 might be a multiple stress-inducible gene responding to hormones and salt stresses”
e1“Panax ginseng”
The same questions in other papers
(-)-methyl jasmonate · molecular: expression · Panax ginseng (ginseng)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
salicylic acid · molecular: expression · Panax ginseng (ginseng)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.