Paper · 2004 · OhioLink ETD Center (Ohio Library and Information Network)

Controlling seedling height by treating seeds with plant growth regulators

Stanislav Magnitskiy

20
claims
23
verbatim quotes
3
citations
3
research areas
✓
Open access

Abstract

Treating seeds with growth regulators may be a suitable method for controlling plug height of selected ornamental and horticultural plants.Soaking seeds in 50-1000 mgL -1 paclobutrazol solutions reduced plug growth in verbena (Verbena x hybrida Voss., cv.Quartz White), pansy (Viola wittrockiana, cv.Bingo Yellow Blotch), salvia (Salvia splendens, cv.Vista Red), marigold (Tagetes patula L., cv.Bonanza Gold), dill (Anathemum graveolens, cv.Fernleaf), and cucumber (Cucumis sativus, cv.Poinsett 76SR).When treating seeds with growth regulators, seedling emergence was delayed and reduced in verbena, pansy, salvia, dill, but not cucumber or marigold.Osmopriming or coating verbena seeds with 10-500 mgL -1 paclobutrazol were associated with 30-93% or 86-100% reduction in seedling emergence, respectively.Soaking marigold seeds during 0.15-45 min in 1-5 mgL -1 uniconazole solutions resulted in plants which were shorter up to 23%, while similar application of 10-60 mgL -1 ancymidol solutions resulted in plants which were shorter up to 6%.Soaking marigold seeds in 1-5 mgL -1 uniconazole or 10-60 mgL -1 ancymidol solutions did not reduce seedling emergence.Storing paclobutrazolsoaked seeds in a cold chamber for 22 months did not affect marigold growth reduction rates and seedling emergence.Soaking celosia (Celosia cristata, cv.New Look) seeds in 50-1000 mgL -1 paclobutrazol solutions was associated with negligible reduction in seedling growth, while similar application of ancymidol or chlormequat did not reduce seedling growth.iii Soaking verbena or marigold seeds in 10-500 mgL -1 paclobutrazol solutions for 5-180 min did not reduce seed viability (tetrazolium test), but did reduce respiration and heat production rates in seeds.Reductions in seedling emergence and reduced respiration rates in paclobutrazol-treated seeds indicate that paclobutrazol imposed dormancy in verbena seeds.Washing restored rates of respiration and heat production in verbena and marigold seeds.Seed coat morphology or hilum size in verbena, celosia, marigold, or cucumber could not be associated with seedling growth response to treating seeds with growth regulators.Scanning electron microscopy found deposits of Bonzi formulation on the surface of celosia seeds that were soaked in 500 mgL -1 paclobutrazol solution for 180 min.Energy disperse x-ray spectroscopy detected increased aluminum concentration on the surface of seeds soaked in 500 (celosia and marigold) or 1000 (cucumber) mgL -1 paclobutrazol solution for 180 min.Paclobutrazol concentration in the seed coats was higher than in the rest of the seed after soaking cucumber seeds in 1000-4000 mgL -1 paclobutrazol solutions.Seed coats of cucumber represent a barrier for paclobutrazol penetration into seeds at least during short soaking durations (5 min).Flowering delays of 7-10 days were found for salvia and cucumber plants grown from seeds soaked in 10-500 (salvia) or 50-1000 (cucumber) mgL -1 paclobutrazol solutions.Soaking salvia seeds in 10-500 mgL -1 paclobutrazol solutions for 5-360 min did not influence germination of the next generation of seeds.Cucumber plants grown from seeds soaked in 1000 mgL -1 paclobutrazol solutions had up to 37% lower average fruit weight than controls.Total fruit yield and fruit size in cucumber were reduced when fruits were collected on plants grown from seeds soaked in increasing paclobutrazol iv rates for 180 min.No reductions in average fruit weight, total fruit yield or fruit diameter were found for tomato (Lycopersicon esculentum Mill., cv.Sun 6108) plants grown from seeds soaked in 50-1000 mgL -1 paclobutrazol solutions for 5-180 min.Paclobutrazol residue was not detected (0.025 gkg -1 DW detection limit) in cucumber or tomato fruits harvested on plants grown from seeds soaked in 1000 mgL -1 paclobutrazol solution during 180 min.From a practical point of view, soaking seeds in 50-500 mgL -1 paclobutrazol solutions for 5-180 min could be employed to produce short cucumber plugs without reducing fruit yield.Results of this study could be further used towards the authorization of paclobutrazol usage for controlling plug height in vegetable crops.

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Claims 20

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking seeds in 50-1000 mg/L paclobutrazol reduced plug growth in verbena, pansy and salvia.

Taxon
verbena (Verbena x hybrida Voss., cv.Quartz White), pansy (Viola wittrockiana, cv.Bingo Yellow Blotch), salvia (Salvia splendens, cv.Vista Red)Glandularia ×hybridaSalvia splendensViola ×wittrockianae0
Treatment
Soaking seeds in 50-1000 mgL -1 paclobutrazol solutionse0
Outcome
plug growthe0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glandularia x hybrida
Glandularia ×hybrida · TaxonWo st 01 · CC BY-SA 4.0 · iNaturalist ↗
Salvia splendens
Salvia splendens · TaxonLucky7879613459 · CC BY-SA 4.0 · Wikimedia Commons ↗
Viola x wittrockiana
Viola ×wittrockiana · Taxon · picture from its Wikimedia Commons categoryAndrew Bossi · CC BY-SA 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Soaking seeds in 50-1000 mgL -1 paclobutrazol solutions reduced plug growth in verbena (Verbena x hybrida Voss., cv.Quartz White), pansy (Viola wittrockiana, cv.Bingo Yellow Blotch), salvia (Salvia splendens, cv.Vista Red)”

✓ located verbatim in the source · abstract, characters 139–361 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking seeds in 50-1000 mg/L paclobutrazol reduced plug growth in marigold, dill and cucumber.

Taxon
marigold (Tagetes patula L., cv.Bonanza Gold), dill (Anathemum graveolens, cv.Fernleaf), and cucumber (Cucumis sativus, cv.Poinsett 76SR)Cucumis sativusAnethum graveolensTagetes patulae1
Treatment
Soaking seeds in 50-1000 mgL -1 paclobutrazol solutionse0
Outcome
plug growthe0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Anethum graveolens
Anethum graveolens · TaxonTepeyac · CC BY-SA 3.0 · Wikimedia Commons ↗
Tagetes patula
Tagetes patula · Taxon · picture from its Wikimedia Commons categoryTomekgg08 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Soaking seeds in 50-1000 mgL -1 paclobutrazol solutions reduced plug growth in”

✓ located verbatim in the source · abstract, characters 139–217 · show in text ↑
e1

“marigold (Tagetes patula L., cv.Bonanza Gold), dill (Anathemum graveolens, cv.Fernleaf), and cucumber (Cucumis sativus, cv.Poinsett 76SR)”

✓ located verbatim in the source · abstract, characters 363–500 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Treating seeds with growth regulators delayed and reduced seedling emergence in verbena, pansy, salvia and dill.

Taxon
verbena, pansy, salvia, dille0
Treatment
treating seeds with growth regulatorse0
Outcome
seedling emergencee0
Direction
delayed and reducede0

Not reportedComparator

Verbatim

e0

“When treating seeds with growth regulators, seedling emergence was delayed and reduced in verbena, pansy, salvia, dill, but not cucumber or marigold”

✓ located verbatim in the source · abstract, characters 501–649 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Treating seeds with growth regulators did not affect seedling emergence in cucumber or marigold.

Taxon
cucumber or marigolde0
Treatment
treating seeds with growth regulatorse0
Outcome
seedling emergencee0
Direction
no_changeinferrede0

Not reportedComparator

Verbatim

e0

“When treating seeds with growth regulators, seedling emergence was delayed and reduced in verbena, pansy, salvia, dill, but not cucumber or marigold”

✓ located verbatim in the source · abstract, characters 501–649 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Osmopriming verbena seeds with 10-500 mg/L paclobutrazol reduced seedling emergence by 30-93%.

Taxon
verbenaVerbenae0
Treatment
Osmopriming or coating verbena seeds with 10-500 mgL -1 paclobutrazole0
Outcome
seedling emergencee0
Direction
reductione0
Value
30-93%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbena
Verbena · Taxon · picture from its Wikimedia Commons categoryRohitverma9625 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Osmopriming or coating verbena seeds with 10-500 mgL -1 paclobutrazol were associated with 30-93% or 86-100% reduction in seedling emergence, respectively”

✓ located verbatim in the source · abstract, characters 650–804 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Coating verbena seeds with 10-500 mg/L paclobutrazol reduced seedling emergence by 86-100%.

Taxon
verbenaVerbenae0
Treatment
Osmopriming or coating verbena seeds with 10-500 mgL -1 paclobutrazole0
Outcome
seedling emergencee0
Direction
reductione0
Value
86-100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbena
Verbena · Taxon · picture from its Wikimedia Commons categoryRohitverma9625 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Osmopriming or coating verbena seeds with 10-500 mgL -1 paclobutrazol were associated with 30-93% or 86-100% reduction in seedling emergence, respectively”

✓ located verbatim in the source · abstract, characters 650–804 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking marigold seeds for 0.15-45 min in 1-5 mg/L uniconazole gave plants up to 23% shorter.

Taxon
marigolde0
Treatment
Soaking marigold seeds during 0.15-45 min in 1-5 mgL -1 uniconazole solutionse0
Outcome
plants which were shortere0
Direction
shortere0
Value
up to 23%e0

Not reportedComparator

Verbatim

e0

“Soaking marigold seeds during 0.15-45 min in 1-5 mgL -1 uniconazole solutions resulted in plants which were shorter up to 23%”

✓ located verbatim in the source · abstract, characters 805–930 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking marigold seeds in 10-60 mg/L ancymidol gave plants up to 6% shorter.

Taxon
marigolde0
Treatment
similar application of 10-60 mgL -1 ancymidol solutionse1
Outcome
plants which were shortere1
Direction
shortere1
Value
up to 6%e1

Not reportedComparator

Verbatim

e0

“Soaking marigold seeds during”

✓ located verbatim in the source · abstract, characters 805–834 · show in text ↑
e1

“similar application of 10-60 mgL -1 ancymidol solutions resulted in plants which were shorter up to 6%”

✓ located verbatim in the source · abstract, characters 938–1040 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Soaking marigold seeds in uniconazole or ancymidol at the tested concentrations did not reduce seedling emergence.

Taxon
marigolde0
Treatment
Soaking marigold seeds in 1-5 mgL -1 uniconazole or 10-60 mgL -1 ancymidol solutionse0
Outcome
seedling emergencee0
Direction
did not reducee0

Not reportedComparator

Verbatim

e0

“Soaking marigold seeds in 1-5 mgL -1 uniconazole or 10-60 mgL -1 ancymidol solutions did not reduce seedling emergence”

✓ located verbatim in the source · abstract, characters 1041–1159 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Storing paclobutrazol-soaked marigold seeds in a cold chamber for 22 months did not change growth reduction rates or seedling emergence.

Taxon
marigolde0
Treatment
Storing paclobutrazolsoaked seeds in a cold chamber for 22 monthse0
Outcome
growth reduction rates and seedling emergencee0
Direction
did not affecte0

Not reportedComparator

Verbatim

e0

“Storing paclobutrazolsoaked seeds in a cold chamber for 22 months did not affect marigold growth reduction rates and seedling emergence”

✓ located verbatim in the source · abstract, characters 1160–1295 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Soaking celosia seeds in 50-1000 mg/L paclobutrazol gave negligible reduction in seedling growth, and ancymidol or chlormequat did not reduce it.

Taxon
Celosia cristataCelosia cristatae0
Material
cv.New Looke0
Treatment
50-1000 mgL -1 paclobutrazol solutionse0
Outcome
seedling growthe0
Direction
negligible reductione0
Qualifier
ancymidol or chlormequat did not reduce seedling growthe0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Celosia cristata
Celosia cristata · TaxonThe original uploader was Earth at English Wikipedia . · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“Soaking celosia (Celosia cristata, cv.New Look) seeds in 50-1000 mgL -1 paclobutrazol solutions was associated with negligible reduction in seedling growth, while similar application of ancymidol or chlormequat did not reduce seedling growth”

✓ located verbatim in the source · abstract, characters 1296–1537 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Soaking verbena or marigold seeds in 10-500 mg/L paclobutrazol for 5-180 min did not reduce seed viability.

Taxon
verbena or marigolde0
Treatment
10-500 mgL -1 paclobutrazol solutions for 5-180 mine0
Outcome
seed viabilitye0
Direction
did not reducee0
Qualifier
tetrazolium teste0

Not reportedComparator

Verbatim

e0

“Soaking verbena or marigold seeds in 10-500 mgL -1 paclobutrazol solutions for 5-180 min did not reduce seed viability (tetrazolium test)”

✓ located verbatim in the source · abstract, characters 1542–1679 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The same paclobutrazol soaks reduced respiration and heat production rates of verbena and marigold seeds.

Taxon
verbena or marigolde0
Treatment
10-500 mgL -1 paclobutrazol solutions for 5-180 mine0
Outcome
respiration and heat production ratese1
Direction
reducee1

Not reportedComparator

Verbatim

e0

“Soaking verbena or marigold seeds in 10-500 mgL -1 paclobutrazol solutions for 5-180 min”

✓ located verbatim in the source · abstract, characters 1542–1630 · show in text ↑
e1

“did reduce respiration and heat production rates in seeds”

✓ located verbatim in the source · abstract, characters 1685–1742 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors infer that paclobutrazol imposed dormancy in verbena seeds, from reduced emergence and respiration.

Taxon
verbenaVerbenae0
Treatment
paclobutrazole0
Outcome
dormancye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbena
Verbena · Taxon · picture from its Wikimedia Commons categoryRohitverma9625 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“paclobutrazol imposed dormancy in verbena seeds”

✓ located verbatim in the source · abstract, characters 1851–1898 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Plants of salvia and cucumber grown from paclobutrazol-soaked seeds flowered 7-10 days later.

Taxon
salvia and cucumberCucumis sativusSalviae0
Treatment
seeds soaked in 10-500 (salvia) or 50-1000 (cucumber) mgL -1 paclobutrazol solutionse0
Outcome
Floweringe0
Direction
delayse0
Value
7-10 dayse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Salvia
Salvia · Taxon · picture of the genus (one of its species)Rob Hille · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Flowering delays of 7-10 days were found for salvia and cucumber plants grown from seeds soaked in 10-500 (salvia) or 50-1000 (cucumber) mgL -1 paclobutrazol solutions”

✓ located verbatim in the source · abstract, characters 2813–2980 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cucumber plants from seeds soaked in 1000 mg/L paclobutrazol had up to 37% lower average fruit weight than controls.

Taxon
CucumberCucumis sativuse0
Treatment
seeds soaked in 1000 mgL -1 paclobutrazol solutionse0
Outcome
average fruit weighte0
Direction
lowere0
Value
up to 37%e0
Comparator
controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Cucumber plants grown from seeds soaked in 1000 mgL -1 paclobutrazol solutions had up to 37% lower average fruit weight than controls”

✓ located verbatim in the source · abstract, characters 3119–3252 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Total fruit yield and fruit size of cucumber were reduced for plants grown from seeds soaked for 180 min in increasing paclobutrazol rates.

Taxon
cucumberCucumis sativuse0
Treatment
seeds soaked in increasing paclobutrazol iv rates for 180 mine0
Outcome
Total fruit yield and fruit sizee0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Total fruit yield and fruit size in cucumber were reduced when fruits were collected on plants grown from seeds soaked in increasing paclobutrazol iv rates for 180 min”

✓ located verbatim in the source · abstract, characters 3253–3420 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Tomato plants grown from seeds soaked in 50-1000 mg/L paclobutrazol for 5-180 min showed no reduction in average fruit weight, total yield or fruit diameter.

Material
cv.Sun 6108e0
Treatment
seeds soaked in 50-1000 mgL -1 paclobutrazol solutions for 5-180 mine0
Outcome
average fruit weight, total fruit yield or fruit diametere0
Direction
No reductionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No reductions in average fruit weight, total fruit yield or fruit diameter were found for tomato (Lycopersicon esculentum Mill., cv.Sun 6108) plants grown from seeds soaked in 50-1000 mgL -1 paclobutrazol solutions for 5-180 min”

✓ located verbatim in the source · abstract, characters 3421–3649 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

No paclobutrazol residue was detected in cucumber or tomato fruits from plants grown from seeds soaked in 1000 mg/L paclobutrazol for 180 min.

Treatment
seeds soaked in 1000 mgL -1 paclobutrazol solution during 180 mine0
Outcome
Paclobutrazol residuee0
Qualifier
0.025 gkg -1 DW detection limite0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Paclobutrazol residue was not detected (0.025 gkg -1 DW detection limit) in cucumber or tomato fruits harvested on plants grown from seeds soaked in 1000 mgL -1 paclobutrazol solution during 180 min”

✓ located verbatim in the source · abstract, characters 3650–3848 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend soaking seeds in 50-500 mg/L paclobutrazol for 5-180 min to produce short cucumber plugs without reducing fruit yield.

Taxon
cucumberCucumis sativuse0
Treatment
soaking seeds in 50-500 mgL -1 paclobutrazol solutions for 5-180 mine0
Outcome
produce short cucumber plugs without reducing fruit yielde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“soaking seeds in 50-500 mgL -1 paclobutrazol solutions for 5-180 min could be employed to produce short cucumber plugs without reducing fruit yield”

✓ located verbatim in the source · abstract, characters 3881–4028 · show in text ↑

paclobutrazol · yield · Cucumis sativus (cucumber)Results differ

5 papers: 3 positive, 2 negative or no effect

1 paper in the same direction as this paper · 3 in the opposite direction

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

paclobutrazol · yield · Solanum lycopersicum (tomato)Results differ

12 papers: 6 positive, 6 negative or no effect

5 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

8 papers more in this group

See all 12 papers →

paclobutrazol · growth · Tagetes (marigolds)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

paclobutrazol · phenology · Cucumis sativus (cucumber)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. positive
See all 2 papers →

paclobutrazol · growth · Verbena (vervains)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

uniconazole · growth · Tagetes (marigolds)Positive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
See all 3 papers →

paclobutrazol · growth · Cucumis sativus (cucumber)Positive and negative results

8 papers: 6 positive, 2 negative or no effect

5 papers in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive

4 papers more in this group

See all 8 papers →

paclobutrazol · growth · Anethum graveolens (dill)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

8 of 25 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.