Abstract
Cell cycle control must be modified at meiosis to allow two divisions to follow a single round of DNA replication, resulting in ploidy reduction. The mechanisms that ensure meiosis termination at the end of the second and not at the end of first division are poorly understood. We show here that Arabidopsis thaliana TDM1, which has been previously shown to be essential for meiotic termination, interacts directly with the Anaphase-Promoting Complex. Further, mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17) dominantly provoked premature meiosis termination after the first division, and the production of diploid spores and gametes. The CDKA;1-CYCA1.2/TAM complex, which is required to prevent premature meiotic exit, phosphorylated TDM1 at T16 in vitro. Finally, while CYCA1;2/TAM was previously shown to be expressed only at meiosis I, TDM1 is present throughout meiosis. These data, together with epistasis analysis, lead us to propose that TDM1 is an APC/C component whose function is to ensure meiosis termination at the end of meiosis II, and whose activity is inhibited at meiosis I by CDKA;1-TAM-mediated phosphorylation to prevent premature meiotic exit. This provides a molecular mechanism for the differential decision of performing an additional round of division, or not, at the end of meiosis I and II, respectively.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Arabidopsis TDM1 directly interacts with the Anaphase-Promoting Complex.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Gene
- TDM1e0
- Outcome
- interacts directly with the Anaphase-Promoting Complexe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis thaliana TDM1, which has been previously shown to be essential for meiotic termination, interacts directly with the Anaphase-Promoting Complex”
Claim · model-written summary
TDM1 T16-P17 mutations caused premature termination after the first meiotic division in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17)e0
- Gene
- TDM1e0
- Outcome
- premature meiosis termination after the first divisione0
- Direction
- provokede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17) dominantly provoked premature meiosis termination after the first division”
e1“Arabidopsis thaliana TDM1”
Claim · model-written summary
TDM1 T16-P17 mutations produced diploid spores and gametes in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17)e0
- Gene
- TDM1e0
- Outcome
- production of diploid spores and gametese0
- Direction
- productione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in TDM1 in a conserved putative Cyclin-Dependant Kinase (CDK) phosphorylation site (T16-P17) dominantly provoked premature meiosis termination after the first division, and the production of diploid spores and gametes”
e1“Arabidopsis thaliana TDM1”
Claim · model-written summary
The CDKA;1-CYCA1.2/TAM complex phosphorylated Arabidopsis TDM1 at T16 in vitro.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- CDKA;1-CYCA1.2/TAM complexe0
- Outcome
- phosphorylated TDM1 at T16e0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The CDKA;1-CYCA1.2/TAM complex, which is required to prevent premature meiotic exit, phosphorylated TDM1 at T16 in vitro”
e1“Arabidopsis thaliana TDM1”
Claim · model-written summary
TDM1 is present throughout meiosis in Arabidopsis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- TDM1e0
- Outcome
- present throughout meiosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TDM1 is present throughout meiosis”
e1“Arabidopsis thaliana TDM1”
Claim · model-written summary
The authors propose that TDM1 ensures meiotic termination at the end of meiosis II as an APC/C component.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- TDM1e0
- Outcome
- ensure meiosis termination at the end of meiosis IIe0
- Qualifier
- lead us to proposee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These data, together with epistasis analysis, lead us to propose that TDM1 is an APC/C component whose function is to ensure meiosis termination at the end of meiosis II”
e1“Arabidopsis thaliana TDM1”
Claim · model-written summary
The authors propose that CDKA;1-TAM-mediated phosphorylation inhibits TDM1 activity at meiosis I to prevent premature meiotic exit.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- TDM1e1
- Outcome
- premature meiotic exite0
- Direction
- inhibitede0
- Qualifier
- at meiosis Ie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whose activity is inhibited at meiosis I by CDKA;1-TAM-mediated phosphorylation to prevent premature meiotic exit”
e1“Arabidopsis thaliana TDM1”
The same questions in other papers
climate site · yield · Arabidopsis thaliana (thale cress)Positive and negative results
1 in the opposite direction
- positive
loss of function · yield · Arabidopsis thaliana (thale cress)Positive and negative results
12 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
14 papers more in this group
See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.