Abstract
The stomatal apparatus consists of a pair of guard cells and regulates gas exchange between the leaf and atmosphere. In guard cells, blue light (BL) activates H(+)-ATPase in the plasma membrane through the phosphorylation of its penultimate threonine, mediating stomatal opening. Although this regulation is thought to be widely adopted among kidney-shaped guard cells in dicots, the molecular basis underlying that of dumbbell-shaped guard cells in monocots remains unclear. Here, we show that H(+)-ATPases are involved in the regulation of dumbbell-shaped guard cells. Stomatal opening of rice was promoted by the H(+)-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H(+)-ATPase inhibitor vanadate. Using H(+)-ATPase antibodies, we showed the presence of phosphoregulation of the penultimate threonine in Oryza sativa H(+)-ATPases (OSAs) and localization of OSAs in the plasma membrane of guard cells. Interestingly, we identified one H(+)-ATPase isoform, OSA7, that is preferentially expressed among the OSA genes in guard cells, and found that loss of function of OSA7 resulted in partial insensitivity to BL. We conclude that H(+)-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species.
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Claims 8
Claim · model-written summary
Fusicoccin promoted rice stomatal opening.
- Taxon
- rice
Oryza sativae0 - Treatment
- fusicoccine0
- Gene
- H(+)-ATPasee0
- Outcome
- Stomatal openinge0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomatal opening of rice was promoted by the H(+)-ATPase activator fusicoccin”
Claim · model-written summary
Blue light promoted rice stomatal opening.
- Taxon
- riceOryza sativae0
- Treatment
- BLe0
- Outcome
- Stomatal openinge0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomatal opening of rice was promoted by the H(+)-ATPase activator fusicoccin and by BL”
Claim · model-written summary
Vanadate suppressed blue-light-promoted rice stomatal opening.
- Taxon
- riceOryza sativae0
- Treatment
- vanadatee0
- Outcome
- Stomatal openinge1
- Direction
- suppressede0
- Comparator
- BLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice was promoted by the H(+)-ATPase activator fusicoccin and by BL, and the latter was suppressed by the H(+)-ATPase inhibitor vanadate”
e1“Stomatal opening of rice”
Claim · model-written summary
Rice OSAs showed phosphoregulation of the penultimate threonine.
- Taxon
- Oryza sativaOryza sativae0
- Gene
- Oryza sativa H(+)-ATPases (OSAs)e1
- Outcome
- phosphoregulation of the penultimate threoninee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa H(+)-ATPases (OSAs)”
e1“presence of phosphoregulation of the penultimate threonine in Oryza sativa H(+)-ATPases (OSAs)”
Claim · model-written summary
Rice OSAs were localized to the guard-cell plasma membrane.
- Taxon
- Oryza sativaOryza sativae0
- Organ / stage
- guard cellse1
- Gene
- OSAse1
- Outcome
- localization of OSAs in the plasma membrane of guard cellse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa H(+)-ATPases (OSAs)”
e1“localization of OSAs in the plasma membrane of guard cells”
Claim · model-written summary
OSA7 was preferentially expressed among OSA genes in guard cells.
- Taxon
- Oryza sativaOryza sativae0
- Organ / stage
- guard cellse1
- Gene
- OSA7e1
- Outcome
- preferentially expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa H(+)-ATPases (OSAs)”
e1“OSA7, that is preferentially expressed among the OSA genes in guard cells”
Claim · model-written summary
Loss of OSA7 function caused partial insensitivity to blue light.
- Taxon
- Oryza sativaOryza sativae0
- Treatment
- loss of function of OSA7e1
- Gene
- OSA7e1
- Outcome
- insensitivity to BLe1
- Qualifier
- partiale1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Oryza sativa H(+)-ATPases (OSAs)”
e1“loss of function of OSA7 resulted in partial insensitivity to BL”
Claim · model-written summary
The authors conclude that H(+)-ATPase participates in blue-light-induced stomatal opening in monocot guard cells.
- Taxon
- riceOryza sativae0
- Organ / stage
- dumbbell-shaped guard cellse1
- Gene
- H(+)-ATPasee1
- Outcome
- BL-induced stomatal openinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stomatal opening of rice”
e1“H(+)-ATPase is involved in BL-induced stomatal opening of dumbbell-shaped guard cells in monocotyledon species”
The same questions in other papers
light · physiology: gas exchange · Oryza sativa (rice)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.