Abstract
Bacillus pumilus MTCC7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to Rhizoctonia solani, the pathogen causing sheath blight disease of rice. The phenazine-1-carboxylic acid gene (phc CD) of this bacterium was PCR amplified (1400 bp), cloned, and sequenced. The sequence analysis revealed the presence of two ORFs of phc CD gene commonly found in Pseudomonas species. The sequence showed 98% similarity to phc CD gene of the Pseudomonas isolate LBUM223 (DQ788993). The crude antibiotic extract from B. pumilus MTCC7615 was observed to inhibit mycelial growth of R. solani under in vitro conditions. The HPLC analysis of crude antibiotic extract from B. pumilus MTCC7615 confirmed the presence of phenazine. The study has also reported the presence of phc CD gene which is responsible for the synthesis of phenazine-1-carboxylic acid in B. pumilus. The ability of the bacterial isolate to control sheath blight disease in rice seedlings under in vivo conditions was confirmed by the pot culture experiment. The structural and functional genomics of phc C and phc D genes would lead to a better understanding of phenazine biosynthesis in B. pumilus for its efficient utilization in plant protection strategies.
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Claims 5
Claim · model-written summary
The crude antibiotic extract from Bacillus pumilus MTCC7615 inhibited mycelial growth of Rhizoctonia solani in vitro.
- Taxon
- rice
Oryza sativae0 - Treatment
- crude antibiotic extract from B. pumilus MTCC7615e1
- Interactor
- R. solani
Rhizoctonia solanie1pathogen - Second interactor
- B. pumilus MTCC7615Bacillus pumiluse1biocontrol_agente0
- Outcome
- mycelial growthe1
- Direction
- inhibite1
- Setting
- in vitro conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus pumilus MTCC7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to Rhizoctonia solani”
e1“The crude antibiotic extract from B. pumilus MTCC7615 was observed to inhibit mycelial growth of R. solani under in vitro conditions”
Claim · model-written summary
HPLC analysis confirmed the presence of phenazine in the crude antibiotic extract of B. pumilus MTCC7615.
- Taxon
- riceOryza sativae0
- Compound
- phenazinee2
- Outcome
- presence of phenazinee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus pumilus MTCC7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to Rhizoctonia solani”
e2“The HPLC analysis of crude antibiotic extract from B. pumilus MTCC7615 confirmed the presence of phenazine”
Claim · model-written summary
The phc CD gene of B. pumilus is responsible for the synthesis of phenazine-1-carboxylic acid.
- Taxon
- riceOryza sativae0
- Gene
- phc CDe3
- Compound
- phenazine-1-carboxylic acide3
- Outcome
- synthesis of phenazine-1-carboxylic acide3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus pumilus MTCC7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to Rhizoctonia solani”
e3“The study has also reported the presence of phc CD gene which is responsible for the synthesis of phenazine-1-carboxylic acid in B. pumilus”
Claim · model-written summary
The phc CD gene sequence showed 98% similarity to the phc CD gene of Pseudomonas isolate LBUM223.
- Taxon
- riceOryza sativae0
- Gene
- phc CDe4
- Outcome
- similaritye4
- Value
- 98%e4
- Comparator
- phc CD gene of the Pseudomonas isolate LBUM223e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus pumilus MTCC7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to Rhizoctonia solani”
e4“The sequence showed 98% similarity to phc CD gene of the Pseudomonas isolate LBUM223 (DQ788993)”
Claim · model-written summary
The bacterial isolate controlled sheath blight disease in rice seedlings under in vivo pot culture conditions.
- Taxon
- riceOryza sativae5
- Treatment
- bacterial isolatee5
- Interactor
- Rhizoctonia solaniRhizoctonia solanie0pathogen
- Outcome
- sheath blight diseasee5
- Direction
- controle5
- Setting
- pot culturee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bacillus pumilus MTCC7615, a biocontrol agent isolated from rice rhizosphere was characterized to be antagonistic to Rhizoctonia solani”
e5“The ability of the bacterial isolate to control sheath blight disease in rice seedlings under in vivo conditions was confirmed by the pot culture experiment”
The same questions in other papers
Bacillus pumilus · Rhizoctonia solani · Oryza sativa (rice)Results agree
1 paper in the same direction as this paper
- positive
antimicrobial agent · Rhizoctonia solani · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
- positive
- positive
botanical · Rhizoctonia solani · Oryza sativa (rice)Positive and negative results
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- no effect
- negative
- positive
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See all 21 papers →inoculation · Rhizoctonia solani · Oryza sativa (rice)Positive and negative results
22 papers in the same direction as this paper · 8 in the opposite direction
- no effect
- negative
- negative
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.