Abstract
Cell suspension cultures were established from shoot explants of mature trees of Acer pseudoplatanus L. (sycamore) at a site contaminated by aerial deposition of copper and cadmium from metal processing industry, and from the same species at uncontaminated sites. The responses of cell cultures to elevated metal concentrations in growth media differed markedly according to site of origin. Both Cu and Cd, applied singly at concentrations of 10–15 mg l−1, inhibited growth and were toxic to cultures originating from the uncontaminated sites, but not to cultures from the contaminated site. This metal tolerance trait in the cultures from the contaminated site was stable through repeated sub-culturing. It could also be induced in one culture originating from the reference uncontaminated site, by gradually exposing the culture to increasing concentrations of Cu. A reduced level of metal removal from the media was found in tolerant cultures, compared to non-tolerant cultures. The results of these experiments demonstrate the occurrence of an alteration of gene expression in response to pollution stress, suggesting that metal tolerance may be induced within shoot meristems in vivo. It also represents the first example of non-mycorrhizal adaptation to metal toxicity identified in woody plants.
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Claims 6
Claim · model-written summary
Cu at 10–15 mg l−1 inhibited reference cultures but not polluted-site cultures.
- Taxon
- Acer pseudoplatanus L.
Acer pseudoplatanust0 - Treatment
- Cue0
- Outcome
- growthe0
- Direction
- inhibitede0
- Value
- 10–15 mg l−1e0
- Comparator
- cultures from the contaminated sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both Cu and Cd, applied singly at concentrations of 10–15 mg l−1, inhibited growth and were toxic to cultures originating from the uncontaminated sites, but not to cultures from the contaminated site.”
t0“Acer pseudoplatanus L.”
Claim · model-written summary
Cd at 10–15 mg l−1 inhibited reference cultures but not polluted-site cultures.
- Taxon
- Acer pseudoplatanus L.Acer pseudoplatanust0
- Treatment
- Cde0
- Outcome
- growthe0
- Direction
- inhibitede0
- Value
- 10–15 mg l−1e0
- Comparator
- cultures from the contaminated sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both Cu and Cd, applied singly at concentrations of 10–15 mg l−1, inhibited growth and were toxic to cultures originating from the uncontaminated sites, but not to cultures from the contaminated site.”
t0“Acer pseudoplatanus L.”
Claim · model-written summary
Polluted-site culture metal tolerance persisted through repeated subculturing.
- Taxon
- Acer pseudoplatanus L.Acer pseudoplatanust0
- Outcome
- metal tolerance traite0
- Direction
- stablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This metal tolerance trait in the cultures from the contaminated site was stable through repeated sub-culturing.”
t0“Acer pseudoplatanus L.”
Claim · model-written summary
Gradually increasing Cu induced tolerance in one reference culture.
- Taxon
- Acer pseudoplatanus L.Acer pseudoplatanust0
- Treatment
- increasing concentrations of Cue0
- Outcome
- inducede0
- Direction
- inducede0
- Comparator
- reference uncontaminated sitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It could also be induced in one culture originating from the reference uncontaminated site, by gradually exposing the culture to increasing concentrations of Cu.”
t0“Acer pseudoplatanus L.”
Claim · model-written summary
Tolerant cultures removed less metal from medium than non-tolerant cultures.
- Taxon
- Acer pseudoplatanus L.Acer pseudoplatanust0
- Outcome
- metal removal from the mediae0
- Direction
- reducede0
- Comparator
- non-tolerant culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reduced level of metal removal from the media was found in tolerant cultures, compared to non-tolerant cultures.”
t0“Acer pseudoplatanus L.”
Claim · model-written summary
Authors inferred altered gene expression in response to pollution stress.
- Taxon
- Acer pseudoplatanus L.Acer pseudoplatanust0
- Outcome
- alteration of gene expressione0
- Qualifier
- in response to pollution stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results of these experiments demonstrate the occurrence of an alteration of gene expression in response to pollution stress, suggesting that metal tolerance may be induced within shoot meristems in vivo.”
t0“Acer pseudoplatanus L.”
The same questions in other papers
cadmium atom · growth · Acer pseudoplatanus (sycamore maple)Results agree
1 paper in the same direction as this paper
- no effect
copper atom · growth · Acer pseudoplatanus (sycamore maple)Results agree
2 papers in the same direction as this paper
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.