Abstract
SsCut, which functions as an elicitor, can induce plant immunity. In this study, we utilized Nicotiana benthamiana and virus-induced gene silencing to decrease the expression of > 2,500 genes individually. Using this forward genetics approach, several genes were identified that, when silenced, compromised SsCut-triggered cell death based on a cell death assay. A C2H2-type zinc finger gene was isolated from N. benthamiana. Sequence analysis indicated that the gene encodes a 27 kDa protein with 253 amino acids containing two typical C2H2-type zinc finger domains; this gene was named NbCZF1. We found that SsCut-induced cell death could be inhibited by virus-induced gene silencing of NbCZF1 in N. benthamiana. In addition, SsCut induces stomatal closure, accompanied by reactive oxygen species (ROS) production by NADPH oxidases and nitric oxide (NO) production. NbCZF1-silenced plants showed impaired SsCut-induced stomatal closure, decreased SsCut-induced production of ROS and NO in guard cells and reduced SsCut-induced resistance against Phytophthora nicotianae. Taken together, these results demonstrate that the NbCZF1–ROS–NO pathway mediates multiple SsCut-triggered responses, including stomatal closure, hypersensitive responses and defense-related gene expression. This is the first report describing the function of a C2H2-type zinc finger protein in N. benthamiana.
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Claims 11
Claim · model-written summary
Several silenced genes compromised SsCut-triggered cell death.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- silencede0
- Outcome
- SsCut-triggered cell deathe0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“several genes were identified that, when silenced, compromised SsCut-triggered cell death”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCZF1 encodes a 27 kDa, 253-amino-acid zinc finger protein with two C2H2 domains.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbCZF1e0
- Outcome
- two typical C2H2-type zinc finger domainse0
- Value
- 27 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gene encodes a 27 kDa protein with 253 amino acids containing two typical C2H2-type zinc finger domains; this gene was named NbCZF1”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbCZF1 inhibited SsCut-induced cell death.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- virus-induced gene silencing of NbCZF1e0
- Gene
- NbCZF1e0
- Outcome
- SsCut-induced cell deathe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SsCut-induced cell death could be inhibited by virus-induced gene silencing of NbCZF1 in N. benthamiana”
Claim · model-written summary
SsCut induced stomatal closure.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- SsCute0
- Outcome
- stomatal closuree0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SsCut induces stomatal closure”
t0“Nicotiana benthamiana”
Claim · model-written summary
SsCut induced guard cell ROS production by NADPH oxidases.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- SsCute0
- Gene
- NADPH oxidasese0
- Outcome
- reactive oxygen species (ROS) productione0
- Direction
- productione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SsCut induces stomatal closure, accompanied by reactive oxygen species (ROS) production by NADPH oxidases and nitric oxide (NO) production”
t0“Nicotiana benthamiana”
Claim · model-written summary
SsCut induced NO production.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- SsCute1
- Outcome
- nitric oxide (NO) productione0
- Direction
- productione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitric oxide (NO) production”
t0“Nicotiana benthamiana”
e1“SsCut induces stomatal closure, accompanied by reactive oxygen species (ROS) production by NADPH oxidases and nitric oxide (NO) production”
Claim · model-written summary
NbCZF1 silencing impaired SsCut-induced stomatal closure.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCZF1-silencede0
- Gene
- NbCZF1e0
- Outcome
- SsCut-induced stomatal closuree0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCZF1-silenced plants showed impaired SsCut-induced stomatal closure”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCZF1 silencing decreased SsCut-induced ROS production in guard cells.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Organ / stage
- guard cellse0
- Treatment
- NbCZF1-silencede0
- Gene
- NbCZF1e0
- Outcome
- SsCut-induced production of ROSe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCZF1-silenced plants showed impaired SsCut-induced stomatal closure, decreased SsCut-induced production of ROS and NO in guard cells”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCZF1 silencing decreased SsCut-induced NO production in guard cells.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCZF1-silencede0
- Gene
- NbCZF1e0
- Outcome
- production of ROS and NO in guard cellse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCZF1-silenced plants showed impaired SsCut-induced stomatal closure, decreased SsCut-induced production of ROS and NO in guard cells”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbCZF1 silencing reduced SsCut-induced resistance to Phytophthora nicotianae.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbCZF1-silencede0
- Interactor
- Phytophthora nicotianae
Phytophthora nicotianaee0pathogen - Gene
- NbCZF1e0
- Outcome
- SsCut-induced resistance against Phytophthora nicotianaee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NbCZF1-silenced plants showed impaired SsCut-induced stomatal closure, decreased SsCut-induced production of ROS and NO in guard cells and reduced SsCut-induced resistance against Phytophthora nicotianae”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors infer that the NbCZF1–ROS–NO pathway mediates several SsCut-triggered responses.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbCZF1–ROS–NO pathwaye0
- Outcome
- stomatal closure, hypersensitive responses and defense-related gene expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the NbCZF1–ROS–NO pathway mediates multiple SsCut-triggered responses, including stomatal closure, hypersensitive responses and defense-related gene expression”
t0“Nicotiana benthamiana”
The same questions in other papers
loss of function · cytology · Nicotiana benthamianaPositive and negative results
23 papers in the same direction as this paper · 9 in the opposite direction
- negative
- positive
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