Paper · 2011 · African Journal of Plant Science

Cytogenetic and biochemical investigations to study the response of Vigna radiata to cadmium stress

Sowbiya Muneer; Tabasum N. Qadri; Mahmooduzaffar; et al.

7
claims
14
verbatim quotes
21
citations
2
research areas
✓
Open access

Abstract

A study was carried out on root tips and leaves of Vigna radiata, 15 days old plantlets grown in nutrient Hoagland media were exposed to various levels of cadmium chloride (0.05, 0.10 and 0.50 mM) for 48 and 72 h. The plant tissues were analyzed for mitotic index, chromosomal variations, root-shoot ratio, leaf area, chlorophyll estimation, and the activity of nitrate content, nitrate reductase (NR), soluble protein and proline content. A number of chromosomal variations such as laggard chromosomes, anaphasic bridges, and unidistribution of chromatin material were observed in root tips. A reduction in mitotic index, root-shoot ratio, area of leaf and chlorophyll estimation was observed at all concentrations of cadmium. Moreover, activity of nitrate reductase (NR), nitrate content and soluble protein content was also found to be decreased. However, rate of proline content was found to be increased on increasing the concentration of cadmium. An increase in the levels of proline suggests its possible incorporation in synthesis of the phytochelatins and metallothioneins to sequester and combat Cd-stress. Key words: Vigna radiata, phytochelatins, cytogenetics, cadmium.

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Claims 7

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium exposure produced chromosomal abnormalities in root tips.

Taxon
Vigna radiataVigna radiatat0
Organ / stage
root tipse0
Outcome
laggard chromosomes, anaphasic bridges, and unidistribution of chromatin materiale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A number of chromosomal variations such as laggard chromosomes, anaphasic bridges, and unidistribution of chromatin material were observed in root tips.”

✓ located verbatim in the source · abstract, characters 440–592 · show in text ↑
t0

“Vigna radiata”

✓ located verbatim in the source · title, characters 68–81 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium reduced mitotic index.

Taxon
Vigna radiataVigna radiatat0
Treatment
cadmiume0
Outcome
mitotic indexe0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A reduction in mitotic index, root-shoot ratio, area of leaf and chlorophyll estimation was observed at all concentrations of cadmium.”

✓ located verbatim in the source · abstract, characters 593–727 · show in text ↑
t0

“Vigna radiata”

✓ located verbatim in the source · title, characters 68–81 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium reduced root-shoot ratio and leaf area.

Taxon
Vigna radiataVigna radiatat0
Treatment
cadmiume0
Outcome
root-shoot ratio, area of leafe0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A reduction in mitotic index, root-shoot ratio, area of leaf and chlorophyll estimation was observed at all concentrations of cadmium.”

✓ located verbatim in the source · abstract, characters 593–727 · show in text ↑
t0

“Vigna radiata”

✓ located verbatim in the source · title, characters 68–81 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium reduced chlorophyll.

Taxon
Vigna radiataVigna radiatat0
Treatment
cadmiume0
Outcome
chlorophyll estimatione0
Direction
reductione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A reduction in mitotic index, root-shoot ratio, area of leaf and chlorophyll estimation was observed at all concentrations of cadmium.”

✓ located verbatim in the source · abstract, characters 593–727 · show in text ↑
t0

“Vigna radiata”

✓ located verbatim in the source · title, characters 68–81 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cadmium decreased nitrate reductase activity, nitrate and soluble protein content.

Taxon
Vigna radiataVigna radiatat0
Treatment
cadmiumt0
Outcome
activity of nitrate reductase (NR), nitrate content and soluble protein contente0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“activity of nitrate reductase (NR), nitrate content and soluble protein content was also found to be decreased.”

✓ located verbatim in the source · abstract, characters 738–849 · show in text ↑
t0

“response of Vigna radiata to cadmium stress”

✓ located verbatim in the source · title, characters 56–99 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Proline content increased with cadmium concentration.

Taxon
Vigna radiataVigna radiatat0
Treatment
cadmiume0
Outcome
proline contente0
Direction
increasede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“rate of proline content was found to be increased on increasing the concentration of cadmium.”

✓ located verbatim in the source · abstract, characters 859–952 · show in text ↑
t0

“Vigna radiata”

✓ located verbatim in the source · title, characters 68–81 · show in text ↑
Mechanismhypothesisoriginal resultpositivemeasured

Claim · model-written summary

The authors suggest proline may contribute to phytochelatin and metallothionein synthesis under cadmium stress.

Taxon
Vigna radiataVigna radiatat0
Compound
prolinee0
Outcome
synthesis of the phytochelatins and metallothioneinse0
Qualifier
possible incorporatione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna radiata
Vigna radiata · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“An increase in the levels of proline suggests its possible incorporation in synthesis of the phytochelatins and metallothioneins to sequester and combat Cd-stress.”

✓ located verbatim in the source · abstract, characters 953–1116 · show in text ↑
t0

“Vigna radiata”

✓ located verbatim in the source · title, characters 68–81 · show in text ↑

cadmium atom · physiology: enzyme activity · Vigna radiata (mung bean)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative
See all 4 papers →

cadmium atom · cytology · Vigna radiata (mung bean)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. negative
See all 4 papers →

cadmium atom · physiology: pigments · Vigna radiata (mung bean)Results agree

7 papers: 0 positive, 7 negative or no effect

6 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

3 papers more in this group

See all 7 papers →

cadmium atom · growth · Vigna radiata (mung bean)Results agree

21 papers: 0 positive, 21 negative or no effect

20 papers in the same direction as this paper

  1. negative
  2. negative
  3. negative

17 papers more in this group

See all 21 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.