Abstract
ABSTRACT Somatic embryo induction medium was reported to be genotype dependent for soybean. This study was aimed to obtain the optimum medium for embryo somatic induction and proliferation, and to regenerate somatic embryo of five soybean genotypes. Five soybean genotypes (Tanggamus, Anjasmoro, Yellow Biloxi, CG-22-10, and SP-10-4) were used in this study. The research was divided into four steps: (1) embryogenic callus induction of five soybean genotypes, (2) embryogenic callus proliferation of five soybean genotypes, (3) optimation of embryo somatic induction on five soybean genotypes and (4) embryo somatic regeneration of five soybean genotypes. The induction experiment showed that based on number of embryogenic callus, the best somatic embryo-induction medium was 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5. Embryogenic callus number for each genotype tested was increased on proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5, and Yellow Biloxi gave the highest number of proliferated somatic embryos compared to other genotypes. Increasing number of globular somatic embryo of all genotypes was obtained from the optimation of somatic embryo induction media being used, and Tanggamus genotype gave the highest number of globular somatic embryo which followed by Yellow Biloxi genotype. Tanggamus and Yellow Biloxi genotypes were also successfully formed the four steps of somatic embryos (globular, heart, torpedo, and cotyledonary stages), but in regeneration medium of MS0 and media MS + sukrosa 10 g L-1 + GA3 2 mg L-1 + BAP 4 mg L-1 + Vit B5 only Tanggamus genotype was regenerated into plantlet. Keywords: 2,4-D, NAA, somatic embryos, induction, proliferation
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-sa. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Based on embryogenic callus number, the best somatic embryo-induction medium for soybean was 3% sucrose + NAA 5 mg L-1 + 2,4-D 5 mg L-1 + Vitamin B5.
- Taxon
- soybean
Glycine maxe1 - Treatment
- 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5e0
- Outcome
- number of embryogenic calluse0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“based on number of embryogenic callus, the best somatic embryo-induction medium was 3% sucrose+ NAA 5 mg L-1+2,4-D 5 mg L-1+ Vitamin B5”
e1“Five soybean genotypes”
Claim · model-written summary
Embryogenic callus number of every soybean genotype increased on proliferation medium of 3% sucrose + 2,4-D 5 mg L-1 + NAA 5 mg L-1 + Vitamin B5.
- Taxon
- soybeanGlycine maxe1
- Treatment
- proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5e0
- Outcome
- Embryogenic callus numbere0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic callus number for each genotype tested was increased on proliferation media of 3% sukrosa + 2,4-D 5 mg L-1 + NAA 5 mg L-1+ Vit B5”
e1“Five soybean genotypes”
Claim · model-written summary
Genotype Yellow Biloxi gave the highest number of proliferated somatic embryos compared with the other genotypes.
- Taxon
- soybeanGlycine maxe1
- Material
- Yellow Biloxie0
- Outcome
- number of proliferated somatic embryose0
- Direction
- higheste0
- Comparator
- other genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Yellow Biloxi gave the highest number of proliferated somatic embryos compared to other genotypes”
e1“Five soybean genotypes”
Claim · model-written summary
The number of globular somatic embryos increased for all genotypes after optimization of the induction medium.
- Taxon
- soybeanGlycine maxe1
- Treatment
- optimation of somatic embryo induction mediae0
- Outcome
- number of globular somatic embryoe0
- Direction
- Increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing number of globular somatic embryo of all genotypes was obtained from the optimation of somatic embryo induction media being used”
e1“Five soybean genotypes”
Claim · model-written summary
Genotype Tanggamus gave the highest number of globular somatic embryos, followed by Yellow Biloxi.
- Taxon
- soybeanGlycine maxe1
- Material
- Tanggamuse0
- Outcome
- number of globular somatic embryoe0
- Direction
- higheste0
- Qualifier
- followed by Yellow Biloxi genotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tanggamus genotype gave the highest number of globular somatic embryo which followed by Yellow Biloxi genotype”
e1“Five soybean genotypes”
Claim · model-written summary
Genotypes Tanggamus and Yellow Biloxi formed all four somatic embryo stages (globular, heart, torpedo and cotyledonary).
- Taxon
- soybeanGlycine maxe1
- Material
- Tanggamus and Yellow Biloxie0
- Outcome
- four steps of somatic embryose0
- Qualifier
- globular, heart, torpedo, and cotyledonary stagese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tanggamus and Yellow Biloxi genotypes were also successfully formed the four steps of somatic embryos (globular, heart, torpedo, and cotyledonary stages)”
e1“Five soybean genotypes”
Claim · model-written summary
Only genotype Tanggamus regenerated into a plantlet on the regeneration media tested.
- Taxon
- soybeanGlycine maxe1
- Material
- Tanggamuse0
- Outcome
- regenerated into plantlete0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only Tanggamus genotype was regenerated into plantlet”
e1“Five soybean genotypes”
The same questions in other papers
2,4-D · growth · Glycine max (soybean)Results differ
19 papers in the same direction as this paper · 15 in the opposite direction
- negative
- negative
- negative
31 papers more in this group
See all 35 papers →1-naphthaleneacetic acid · growth · Glycine max (soybean)Positive and negative results
14 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- negative
- negative
15 papers more in this group
See all 19 papers →in vitro · growth · Glycine max (soybean)Positive and negative results
37 papers in the same direction as this paper · 14 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
49 papers more in this group
See all 53 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.