Abstract
Rice blast, caused by Magnaporthe oryzae (synonym: Pyricularia oryzae), severely reduces rice production and grain quality. The molecular mechanism of rice resistance to M. oryzae is not fully understood. In this study, we identified a chaperone DnaJ protein, OsDjA6, which is involved in basal resistance to M. oryzae in rice. The OsDjA6 protein is distributed in the entire rice cell. The expression of OsDjA6 is significantly induced in rice after infection with a compatible isolate. Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments. In addition, the expression levels of WRKY45, NPR1 and PR5 are increased in OsDjA6 RNAi plants, indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway. Finally, we found that OsDjA6 interacts directly with the E3 ligase OsZFP1 in vitro and in vivo. These results suggest that the DnaJ protein OsDjA6 negatively regulates rice innate immunity, probably via the ubiquitination proteasome degradation pathway.
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Claims 10
Claim · model-written summary
OsDjA6 was identified as a DnaJ chaperone involved in rice basal blast resistance.
- Taxon
- rice
Oryza sativae0 - Interactor
- M. oryzae
Pyricularia oryzaee0pathogen - Gene
- OsDjA6e0
- Outcome
- basal resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In this study, we identified a chaperone DnaJ protein, OsDjA6, which is involved in basal resistance to M. oryzae in rice.”
Claim · model-written summary
OsDjA6 is distributed throughout the rice cell.
- Taxon
- riceOryza sativae0
- Gene
- OsDjA6e0
- Outcome
- distributed in the entire rice celle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The OsDjA6 protein is distributed in the entire rice cell.”
Claim · model-written summary
Compatible infection significantly induced rice OsDjA6 expression.
- Taxon
- riceOryza sativae0
- Treatment
- infection with a compatible isolatee0
- Gene
- OsDjA6e0
- Outcome
- expression of OsDjA6e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of OsDjA6 is significantly induced in rice after infection with a compatible isolate.”
Claim · model-written summary
Silencing OsDjA6 enhanced rice resistance to M. oryzae.
- Taxon
- riceOryza sativae0
- Treatment
- Silencing of OsDjA6e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Gene
- OsDjA6e0
- Outcome
- resistance to M. oryzaee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments.”
Claim · model-written summary
OsDjA6 silencing increased the reactive oxygen species burst after flg22 treatment.
- Taxon
- riceOryza sativae0
- Treatment
- Silencing of OsDjA6e0
- Gene
- OsDjA6e0
- Outcome
- burst of reactive oxygen speciese0
- Direction
- increasede0
- Qualifier
- flg22e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments.”
Claim · model-written summary
OsDjA6 silencing increased the reactive oxygen species burst after chitin treatment.
- Taxon
- riceOryza sativae0
- Treatment
- Silencing of OsDjA6e0
- Gene
- OsDjA6e0
- Outcome
- burst of reactive oxygen speciese0
- Direction
- increasede0
- Qualifier
- chitin treatmentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of OsDjA6 in transgenic rice enhances resistance to M. oryzae and also results in an increased burst of reactive oxygen species after flg22 and chitin treatments.”
Claim · model-written summary
OsDjA6 RNAi plants had increased WRKY45, NPR1 and PR5 expression.
- Taxon
- riceOryza sativat0
- Treatment
- OsDjA6 RNAie0
- Outcome
- expression levels of WRKY45, NPR1 and PR5e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In addition, the expression levels of WRKY45, NPR1 and PR5 are increased in OsDjA6 RNAi plants, indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway.”
t0“rice”
Claim · model-written summary
OsDjA6 may mediate rice resistance through the salicylic acid pathway.
- Taxon
- riceOryza sativat0
- Gene
- OsDjA6e0
- Compound
- salicylic acide0
- Outcome
- resistancee0
- Qualifier
- may mediate resistance by affecting the salicylic acid pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating that OsDjA6 may mediate resistance by affecting the salicylic acid pathway.”
t0“rice”
Claim · model-written summary
OsDjA6 directly interacts with OsZFP1 in vitro and in vivo.
- Taxon
- riceOryza sativat0
- Gene
- OsDjA6e0
- Outcome
- interacts directly with the E3 ligase OsZFP1e0
- Qualifier
- in vitro and in vivoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Finally, we found that OsDjA6 interacts directly with the E3 ligase OsZFP1 in vitro and in vivo.”
t0“rice”
Claim · model-written summary
OsDjA6 is suggested to negatively regulate rice immunity, probably through ubiquitination proteasome degradation.
- Taxon
- riceOryza sativae0
- Gene
- OsDjA6e0
- Outcome
- innate immunitye0
- Direction
- negatively regulatese0
- Qualifier
- probably via the ubiquitination proteasome degradation pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that the DnaJ protein OsDjA6 negatively regulates rice innate immunity, probably via the ubiquitination proteasome degradation pathway.”
The same questions in other papers
loss of function · Pyricularia oryzae · Oryza sativa (rice)Positive and negative results
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