Paper · 2013 · Biotechnology : an Indian journal

Effects of salt and drought stress conditions on callus growth, proline content and antioxidant enzyme avtivity of Punica granatum ÃÂNanaÃÂ

Alireza Bonyanpour; Morteza Khosh-Khui

11
claims
22
verbatim quotes
0
citations
3
research areas
—
Not open access

Abstract

Callus of Punica granatum ‘Nana’ were cultured on MS medium supplemented with various concentrations of BA and NAA to evaluate callus growth. The highest callus freshweight (0.61 g), dryweight (0.068 g) and diameter (16.16 mm) were observed in 1 mg L-1 BA and 0.1 mg L-1 NAA. In a separate experiment, growth and antioxidant activity (Super oxidismutase (SOD), Catalase (CAT) and Peroxidase (POD)) of calluswere studied in plants grown under saline (0 -5 g L-1 NaCl) and drought (0 to 8% PEG) conditions. Results showed that under both conditions, growth of callus decreased with increasing in stress treatments. Under saline condition, increasing in salinity increased antioxidant enzyme activity. The highest CAT, POD and SOD activities observed in 4, 5, 2 g L-1 NaCl, respectively.While under drought conditions, the highest SOD and CAT activities observed in 6% PEG and highest POD activity occurred in 8% PEG. The survived tolerant calli obtained had potential to produce salt and drought tolerant cultivars.

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Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest callus fresh weight was observed on 1 mg L-1 BA and 0.1 mg L-1 NAA medium.

Taxon
Punica granatumPunica granatumt0
Organ / stage
calluse1
Treatment
1 mg L-1 BA and 0.1 mg L-1 NAAe1
Outcome
callus freshweighte1
Direction
higheste1
Value
0.61 ge1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“The highest callus freshweight (0.61 g), dryweight (0.068 g) and diameter (16.16 mm) were observed in 1 mg L-1 BA and 0.1 mg L-1 NAA”

✓ located verbatim in the source · abstract, characters 142–274 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest callus dry weight was observed on 1 mg L-1 BA and 0.1 mg L-1 NAA medium.

Taxon
Punica granatumPunica granatumt0
Organ / stage
calluse1
Treatment
1 mg L-1 BA and 0.1 mg L-1 NAAe1
Outcome
dryweighte1
Direction
higheste1
Value
0.068 ge1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“The highest callus freshweight (0.61 g), dryweight (0.068 g) and diameter (16.16 mm) were observed in 1 mg L-1 BA and 0.1 mg L-1 NAA”

✓ located verbatim in the source · abstract, characters 142–274 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The largest callus diameter was observed on 1 mg L-1 BA and 0.1 mg L-1 NAA medium.

Taxon
Punica granatumPunica granatumt0
Organ / stage
calluse1
Treatment
1 mg L-1 BA and 0.1 mg L-1 NAAe1
Outcome
diametere1
Direction
higheste1
Value
16.16 mme1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“The highest callus freshweight (0.61 g), dryweight (0.068 g) and diameter (16.16 mm) were observed in 1 mg L-1 BA and 0.1 mg L-1 NAA”

✓ located verbatim in the source · abstract, characters 142–274 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Salt and drought stress treatments decreased callus growth under both conditions.

Taxon
Punica granatumPunica granatumt0
Organ / stage
calluse2
Treatment
stress treatmentse2
Outcome
growth of calluse2
Direction
decreasede2
Qualifier
under both conditionse2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“Results showed that under both conditions, growth of callus decreased with increasing in stress treatments”

✓ located verbatim in the source · abstract, characters 505–611 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Increasing salinity increased antioxidant enzyme activity in callus under saline condition.

Taxon
Punica granatumPunica granatumt0
Treatment
increasing in salinitye3
Outcome
antioxidant enzyme activitye3
Direction
increasede3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“Under saline condition, increasing in salinity increased antioxidant enzyme activity”

✓ located verbatim in the source · abstract, characters 613–697 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under salinity, CAT activity was highest at 4 g L-1 NaCl.

Taxon
Punica granatumPunica granatumt0
Treatment
NaCle4
Outcome
CATe4
Direction
higheste4
Qualifier
4, 5, 2 g L-1 NaCl, respectivelye4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The highest CAT, POD and SOD activities observed in 4, 5, 2 g L-1 NaCl, respectively”

✓ located verbatim in the source · abstract, characters 699–783 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under salinity, POD activity was highest at 5 g L-1 NaCl.

Taxon
Punica granatumPunica granatumt0
Treatment
NaCle4
Outcome
PODe4
Direction
higheste4
Qualifier
4, 5, 2 g L-1 NaCl, respectivelye4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The highest CAT, POD and SOD activities observed in 4, 5, 2 g L-1 NaCl, respectively”

✓ located verbatim in the source · abstract, characters 699–783 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under salinity, SOD activity was highest at 2 g L-1 NaCl.

Taxon
Punica granatumPunica granatumt0
Treatment
NaCle4
Outcome
SODe4
Direction
higheste4
Qualifier
4, 5, 2 g L-1 NaCl, respectivelye4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The highest CAT, POD and SOD activities observed in 4, 5, 2 g L-1 NaCl, respectively”

✓ located verbatim in the source · abstract, characters 699–783 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under drought, SOD and CAT activities were highest at 6% PEG.

Taxon
Punica granatumPunica granatumt0
Treatment
drought conditionse5
Outcome
SOD and CAT activitiese5
Direction
higheste5
Qualifier
6% PEGe5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“While under drought conditions, the highest SOD and CAT activities observed in 6% PEG and highest POD activity occurred in 8% PEG”

✓ located verbatim in the source · abstract, characters 784–913 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Under drought, POD activity was highest at 8% PEG.

Taxon
Punica granatumPunica granatumt0
Treatment
drought conditionse5
Outcome
POD activitye5
Direction
higheste5
Qualifier
8% PEGe5

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“While under drought conditions, the highest SOD and CAT activities observed in 6% PEG and highest POD activity occurred in 8% PEG”

✓ located verbatim in the source · abstract, characters 784–913 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The surviving tolerant calli have potential to produce salt and drought tolerant cultivars.

Taxon
Punica granatumPunica granatumt0
Outcome
salt and drought tolerant cultivarse6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Punica granatum
Punica granatum · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“The survived tolerant calli obtained had potential to produce salt and drought tolerant cultivars”

✓ located verbatim in the source · abstract, characters 915–1012 · show in text ↑
t0

“Punica granatum”

✓ located verbatim in the source · title, characters 115–130 · show in text ↑

N-benzyladenine · growth · Punica granatum (pomegranate)Results agree

24 papers: 24 positive, 0 negative or no effect

23 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

20 papers more in this group

See all 24 papers →

1-naphthaleneacetic acid · growth · Punica granatum (pomegranate)Results agree

25 papers: 25 positive, 0 negative or no effect

24 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

21 papers more in this group

See all 25 papers →

drought · physiology: antioxidant enzymes · Punica granatum (pomegranate)Results differ

4 papers: 2 positive, 2 negative or no effect

1 paper in the same direction as this paper · 2 in the opposite direction

  1. negative
  2. negative
  3. positive
See all 4 papers →

salinity · soil: biology · Punica granatum (pomegranate)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.