Abstract
BACKGROUND: MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression of target mRNAs involved in plant growth, development, and abiotic stress. As one of the most important model plants, peach (Prunus persica) has high agricultural significance and nutritional values. It is well adapted to be cultivated in greenhouse in which some auxiliary conditions like temperature, humidity, and UVB etc. are needed to ensure the fruit quality. However, little is known about the genomic information of P. persica under UVB supplement. Transcriptome and expression profiling data for this species are therefore important resources to better understand the biological mechanism of seed development, formation and plant adaptation to environmental change. Using a high-throughput miRNA sequencing, followed by qRT-PCR tests and physiological properties determination, we identified the responsive-miRNAs under low-dose UVB treatment and described the expression pattern and putative function of related miRNAs and target genes in chlorophyll and carbohydrate metabolism. RESULTS: A total of 164 known peach miRNAs belonging to 59 miRNA families and 109 putative novel miRNAs were identified. Some of these miRNAs were highly conserved in at least four other plant species. In total, 1794 and 1983 target genes for known and novel miRNAs were predicted, respectively. The differential expression profiles of miRNAs between the control and UVB-supplement group showed that UVB-responsive miRNAs were mainly involved in carbohydrate metabolism and signal transduction. UVB supplement stimulated peach to synthesize more chlorophyll and sugars, which was verified by qRT-PCR tests of related target genes and metabolites' content measurement. CONCLUSION: The high-throughput sequencing data provided the most comprehensive miRNAs resource available for peach study. Our results identified a series of differentially expressed miRNAs/target genes that were predicted to be low-dose UVB-responsive. The correlation between transcriptional profiles and metabolites contents in UVB supplement groups gave novel clues for the regulatory mechanism of miRNAs in Prunus. Low-dose UVB supplement could increase the chlorophyll and sugar (sorbitol) contents via miRNA-target genes and therefore improve the fruit quality in protected cultivation of peaches.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
A total of 164 known peach miRNAs belonging to 59 miRNA families were identified.
- Taxon
- peach
Prunus persicae0 - Outcome
- known peach miRNAse1
- Value
- 164e1
- Qualifier
- belonging to 59 miRNA familiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e1“A total of 164 known peach miRNAs belonging to 59 miRNA families and 109 putative novel miRNAs were identified”
Claim · model-written summary
109 putative novel peach miRNAs were identified.
- Taxon
- peachPrunus persicae0
- Outcome
- putative novel miRNAse1
- Value
- 109e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e1“A total of 164 known peach miRNAs belonging to 59 miRNA families and 109 putative novel miRNAs were identified”
Claim · model-written summary
For known miRNAs, 1794 target genes were predicted.
- Taxon
- peachPrunus persicae0
- Outcome
- target genese2
- Value
- 1794e2
- Qualifier
- for known and novel miRNAse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e2“In total, 1794 and 1983 target genes for known and novel miRNAs were predicted, respectively”
Claim · model-written summary
For novel miRNAs, 1983 target genes were predicted.
- Taxon
- peachPrunus persicae0
- Material
- novel miRNAse2
- Outcome
- target genese2
- Value
- 1983e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e2“In total, 1794 and 1983 target genes for known and novel miRNAs were predicted, respectively”
Claim · model-written summary
Some peach miRNAs were highly conserved in at least four other plant species.
- Taxon
- peachPrunus persicae0
- Outcome
- highly conservede3
- Qualifier
- in at least four other plant speciese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e3“Some of these miRNAs were highly conserved in at least four other plant species”
Claim · model-written summary
UVB-responsive miRNAs were mainly involved in carbohydrate metabolism and signal transduction in the UVB-supplement group compared with control.
- Taxon
- peachPrunus persicae0
- Treatment
- UVB-supplemente4
- Outcome
- carbohydrate metabolism and signal transductione4
- Comparator
- controle4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e4“The differential expression profiles of miRNAs between the control and UVB-supplement group showed that UVB-responsive miRNAs were mainly involved in carbohydrate metabolism and signal transduction”
Claim · model-written summary
UVB supplement stimulated peach to synthesize more chlorophyll and sugars.
- Taxon
- peachPrunus persicae0
- Treatment
- UVB supplemente5
- Outcome
- chlorophyll and sugarse5
- Direction
- stimulatede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e5“UVB supplement stimulated peach to synthesize more chlorophyll and sugars”
Claim · model-written summary
Low-dose UVB supplement could increase chlorophyll and sugar (sorbitol) contents via miRNA-target genes, which the authors suggest would improve fruit quality.
- Taxon
- peachPrunus persicae0
- Treatment
- Low-dose UVB supplemente6
- Outcome
- chlorophyll and sugar (sorbitol) contentse6
- Qualifier
- via miRNA-target genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“As one of the most important model plants, peach (Prunus persica) has high agricultural significance”
e6“Low-dose UVB supplement could increase the chlorophyll and sugar (sorbitol) contents via miRNA-target genes and therefore improve the fruit quality in protected cultivation of peaches”
The same questions in other papers
uv · physiology: pigments · Prunus persica (peach)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.