Abstract
Deciphering signal transduction processes is crucial for understanding how plants sense and respond to environmental changes. Various chemical compounds function as central messengers within deeply intertwined signaling networks. How such compounds act in concert remains to be elucidated. We have developed dual-reporting transcriptionally linked genetically encoded fluorescent indicators (2-in-1-GEFIs) for multiparametric in vivo analyses of the phytohormone abscisic acid (ABA), Ca2+, protons (H+), chloride (anions), the glutathione redox potential, and H2O2. Simultaneous analyses of two signaling compounds in Arabidopsis (Arabidopsis thaliana) roots revealed that ABA treatment and uptake did not trigger rapid cytosolic Ca2+ or H+ dynamics. Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDE, and glutathione disulfide (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca2+, H+, and anion dynamics, but except for GSSG, only weakly affected the cytosolic redox state. Overall, 2-in-1-GEFIs enable complementary, high-resolution in vivo analyses of signaling compound dynamics and facilitate an advanced understanding of the spatiotemporal coordination of signal transduction processes in Arabidopsis.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Dual-reporting indicators enable multiparametric in vivo analysis of several signaling compounds.
- Taxon
- Arabidopsis
Arabidopsist0 - Outcome
- multiparametric in vivo analysese0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We have developed dual-reporting transcriptionally linked genetically encoded fluorescent indicators (2-in-1-GEFIs) for multiparametric in vivo analyses of the phytohormone abscisic acid (ABA), Ca2+, protons (H+), chloride (anions), the glutathione redox potential, and H2O2”
t0“Arabidopsis”
Claim · model-written summary
ABA treatment and uptake did not trigger rapid cytosolic Ca2+ dynamics in Arabidopsis roots.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- rootse0
- Treatment
- ABA treatment and uptakee0
- Outcome
- cytosolic Ca2+ or H+ dynamicse0
- Direction
- did not triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Simultaneous analyses of two signaling compounds in Arabidopsis (Arabidopsis thaliana) roots revealed that ABA treatment and uptake did not trigger rapid cytosolic Ca2+ or H+ dynamics”
Claim · model-written summary
Glutamate treatment induced rapid overlapping cytosolic Ca2+, H+, and anion dynamics.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Glutamatee0
- Outcome
- cytosolic Ca2+, H+, and anion dynamicse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDE, and glutathione disulfide (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca2+, H+, and anion dynamics”
t0“Arabidopsis”
Claim · model-written summary
ATP treatment induced rapid overlapping cytosolic Ca2+, H+, and anion dynamics.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ATPe0
- Outcome
- cytosolic Ca2+, H+, and anion dynamicse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDE, and glutathione disulfide (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca2+, H+, and anion dynamics”
t0“Arabidopsis”
Claim · model-written summary
Arabidopsis PLANT ELICITOR PEPTIDE treatment induced rapid overlapping cytosolic Ca2+, H+, and anion dynamics.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Arabidopsis PLANT ELICITOR PEPTIDEe0
- Outcome
- cytosolic Ca2+, H+, and anion dynamicse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDE, and glutathione disulfide (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca2+, H+, and anion dynamics”
t0“Arabidopsis”
Claim · model-written summary
GSSG treatment induced rapid overlapping cytosolic Ca2+, H+, and anion dynamics.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- glutathione disulfide (GSSG)e0
- Outcome
- cytosolic Ca2+, H+, and anion dynamicse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDE, and glutathione disulfide (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca2+, H+, and anion dynamics”
t0“Arabidopsis”
Claim · model-written summary
Glutamate, ATP, and Arabidopsis PLANT ELICITOR PEPTIDE weakly affected cytosolic redox state.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDEe0
- Outcome
- cytosolic redox statee0
- Direction
- weakly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutamate, ATP, Arabidopsis PLANT ELICITOR PEPTIDE, and glutathione disulfide (GSSG) treatments induced rapid spatiotemporally overlapping cytosolic Ca2+, H+, and anion dynamics, but except for GSSG, only weakly affected the cytosolic redox state”
t0“Arabidopsis”
The same questions in other papers
ATP · molecular: metabolites · ArabidopsisPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →(+)-abscisic acid · nutrient: content · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →glutathione disulfide · molecular: metabolites · ArabidopsisResults agree
1 paper in the same direction as this paper
- positive
resistance inducer · molecular: metabolites · ArabidopsisPositive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.