Abstract
The cultivar ‘G × N-9’ is an almond x peach hybrid rootstock, the main characteristic of which is resistance to several root-knot nematodes (Meloidogyne spp.). Although its propagation by hardwood cutting in the fall is recommended, optimization of ‘G × N-9’ in vitro propagation is currently needed. Thus, the objective of this study was to evaluate the effect of the cytokinins [6-benzylaminopurine (BAP) and Kinetin (KIN)], and the carbohydrate sources (sucrose and sorbitol) on shoot proliferation and elongation, as well as the influence of indole-3-butyric acid (IBA) on in vitro rooting and acclimatization of ‘G × N-9’ rootstock. This study reports enhanced on shoot proliferation and elongation based on the interaction between BAP and KIN. When comparing the effects of sorbitol and sucrose, sorbitol at 15 and 30 mg L−1 was the most effective for the induction of shoot proliferation (5.75 ± 0.19 and 6.39 ± 0.21 shoots per explant, respectively). The highest number of roots per explant was in QL medium supplemented with 0.6 and 1.2 mg L−1 IBA. After 30 days of acclimatization, the treated-plants with 1.2 mg L−1 IBA showed the smallest root length, but the higher number of roots per explant (8.35 ± 0.41). The successful acclimatization of plantlets depended on the use of IBA during in vitro culture, to induce a healthy and well-developed root system.
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Claims 8
Claim · model-written summary
BAP and KIN interaction enhanced shoot proliferation and elongation.
- Taxon
- Peach
Prunus persicat0 - Treatment
- interaction between BAP and KINe0
- Outcome
- shoot proliferation and elongatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced on shoot proliferation and elongation based on the interaction between BAP and KIN”
t0“‘G × N-9’ Peach Rootstock”
Claim · model-written summary
Sorbitol at 15 mg L−1 gave 5.75 ± 0.19 shoots per explant.
- Taxon
- PeachPrunus persicat0
- Treatment
- sorbitol at 15e0
- Outcome
- shoot proliferatione0
- Direction
- most effectivee0
- Value
- 5.75 ± 0.19e0
- Comparator
- sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When comparing the effects of sorbitol and sucrose, sorbitol at 15 and 30 mg L−1 was the most effective for the induction of shoot proliferation (5.75 ± 0.19 and 6.39 ± 0.21 shoots per explant, respectively).”
t0“Peach Rootstock”
Claim · model-written summary
Sorbitol at 30 mg L−1 gave 6.39 ± 0.21 shoots per explant.
- Taxon
- PeachPrunus persicat0
- Treatment
- sorbitol at 15 and 30 mg L−1e0
- Outcome
- shoot proliferatione0
- Direction
- most effectivee0
- Value
- 6.39 ± 0.21 shoots per explante0
- Comparator
- sucrosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When comparing the effects of sorbitol and sucrose, sorbitol at 15 and 30 mg L−1 was the most effective for the induction of shoot proliferation (5.75 ± 0.19 and 6.39 ± 0.21 shoots per explant, respectively).”
t0“Peach Rootstock”
Claim · model-written summary
QL medium with 0.6 mg L−1 IBA yielded the highest root number.
- Taxon
- PeachPrunus persicat0
- Treatment
- QL medium supplemented with 0.6e0
- Outcome
- number of roots per explante0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of roots per explant was in QL medium supplemented with 0.6 and 1.2 mg L−1 IBA.”
t0“Peach Rootstock”
Claim · model-written summary
QL medium with 1.2 mg L−1 IBA yielded the highest root number.
- Taxon
- PeachPrunus persicat0
- Treatment
- 1.2 mg L−1 IBAe0
- Outcome
- number of roots per explante0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest number of roots per explant was in QL medium supplemented with 0.6 and 1.2 mg L−1 IBA.”
t0“Peach Rootstock”
Claim · model-written summary
After 30 days of acclimatization, 1.2 mg L−1 IBA treated plants had the smallest root length.
- Taxon
- PeachPrunus persicat0
- Treatment
- 1.2 mg L−1 IBAe0
- Outcome
- root lengthe0
- Direction
- smalleste0
- Qualifier
- After 30 days of acclimatizatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 30 days of acclimatization, the treated-plants with 1.2 mg L−1 IBA showed the smallest root length, but the higher number of roots per explant (8.35 ± 0.41).”
t0“Peach Rootstock”
Claim · model-written summary
After acclimatization, 1.2 mg L−1 IBA treated plants had more roots per explant, at 8.35 ± 0.41.
- Taxon
- PeachPrunus persicat0
- Treatment
- 1.2 mg L−1 IBAe0
- Outcome
- number of roots per explante0
- Direction
- highere0
- Value
- 8.35 ± 0.41e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 30 days of acclimatization, the treated-plants with 1.2 mg L−1 IBA showed the smallest root length, but the higher number of roots per explant (8.35 ± 0.41).”
t0“Peach Rootstock”
Claim · model-written summary
Successful acclimatization depended on IBA use during in vitro culture.
- Taxon
- PeachPrunus persicat0
- Treatment
- use of IBA during in vitro culturee0
- Outcome
- successful acclimatization of plantletse0
- Direction
- depended one0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The successful acclimatization of plantlets depended on the use of IBA during in vitro culture, to induce a healthy and well-developed root system.”
t0“Peach Rootstock”
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