Abstract
WRKY genes and jasmonic acid (JA) play a crucial role in plants’ responses against biotic and abiotic stress. However, the regulating mechanism of WRKY genes on strawberry fruits’ resistance against Botrytis cinerea is largely unknown, and few studies have been performed on their effect on the JA-mediated defense mechanism against B. cinerea. This study explored the effect of FaWRKY25 on the JA-mediated strawberry resistance against B. cinerea. Results showed that the JA content decreased significantly as the fruits matured, whereas the FaWRKY25 expression rose substantially, which led to heightened susceptibility to B. cinerea and in strawberries. External JA treatment significantly increased the JA content in strawberries and reduced the FaWRKY25 expression, thereby enhancing the fruits’ resistance against B. cinerea. FaWRKY25 overexpression significantly lowered the fruits’ resistance against B. cinerea, whereas FaWRKY25 silencing significantly increased resistance. Moreover, FaWRKY25 overexpression significantly lowered the JA content, whereas FaWRKY25 silencing significantly increased it. FaWRKY25 expression level substantially affects the expression levels of genes related to JA biosynthesis and metabolism, other members of the WRKY family, and defense genes. Accordingly, FaWRKY25 plays a crucial role in regulating strawberries’ resistance against B. cinerea and may negatively regulate their JA-mediated resistance mechanism against B. cinerea.
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Claims 12
Claim · model-written summary
JA content declined significantly as strawberry fruit matured.
- Taxon
- Strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Organ / stage
- fruitse0
- Outcome
- JA contente0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“the JA content decreased significantly as the fruits matured”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 expression rose as strawberry fruit matured.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FaWRKY25e0
- Outcome
- FaWRKY25 expressione0
- Direction
- rosee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the FaWRKY25 expression rose substantially”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
Fruit maturation was accompanied by increased susceptibility to Botrytis.
- Taxon
- StrawberryFragaria ×ananassat0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- susceptibilitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“heightened susceptibility to B. cinerea”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
External JA significantly raised strawberry JA content.
- Taxon
- strawberriesFragaria ×ananassae0
- Treatment
- External JA treatmente0
- Outcome
- JA contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“External JA treatment significantly increased the JA content in strawberries”
Claim · model-written summary
External JA reduced FaWRKY25 expression.
- Taxon
- strawberriesFragaria ×ananassae0
- Treatment
- External JA treatmente0
- Gene
- FaWRKY25e0
- Outcome
- FaWRKY25 expressione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“External JA treatment significantly increased the JA content in strawberries and reduced the FaWRKY25 expression”
Claim · model-written summary
External JA enhanced strawberry fruit resistance to Botrytis.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- External JA treatmente0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- resistance against B. cinereae0
- Direction
- enhancinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“External JA treatment significantly increased the JA content in strawberries and reduced the FaWRKY25 expression, thereby enhancing the fruits’ resistance against B. cinerea”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 overexpression significantly reduced fruit resistance to Botrytis.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- FaWRKY25 overexpressione0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- FaWRKY25e0
- Outcome
- fruits’ resistancee0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaWRKY25 overexpression significantly lowered the fruits’ resistance against B. cinerea”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 silencing significantly increased fruit resistance.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- FaWRKY25 silencinge0
- Gene
- FaWRKY25e0
- Outcome
- resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaWRKY25 silencing significantly increased resistance”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 overexpression significantly lowered JA content.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- FaWRKY25 overexpressione0
- Gene
- FaWRKY25e0
- Outcome
- JA contente0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaWRKY25 overexpression significantly lowered the JA content”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 silencing significantly increased JA content.
- Taxon
- StrawberryFragaria ×ananassat0
- Treatment
- FaWRKY25 silencinge0
- Gene
- FaWRKY25e0
- Outcome
- JA contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaWRKY25 overexpression significantly lowered the JA content, whereas FaWRKY25 silencing significantly increased it”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 expression substantially affected JA pathway, WRKY family and defense gene expression.
- Taxon
- StrawberryFragaria ×ananassat0
- Gene
- FaWRKY25e0
- Outcome
- expression levels of genes related to JA biosynthesis and metabolism, other members of the WRKY family, and defense genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaWRKY25 expression level substantially affects the expression levels of genes related to JA biosynthesis and metabolism, other members of the WRKY family, and defense genes”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
Claim · model-written summary
FaWRKY25 may negatively regulate JA-mediated resistance to Botrytis.
- Taxon
- StrawberryFragaria ×ananassat0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- FaWRKY25t0
- Outcome
- JA-mediated resistance mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“may negatively regulate their JA-mediated resistance mechanism against B. cinerea”
t0“Strawberry FaWRKY25 Transcription Factor Negatively Regulated the Resistance of Strawberry Fruits to Botrytis cinerea”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.