Abstract
SummaryWhether or not βglucosidase is active in fleshy fruit remains unknown. We investigated levels of gene expression, enzyme activities, and the physiological functions of βglucosidase 1 (FaBG1) in strawberry (Fragaria ananassa Duch.) fruit flesh. Real-time RT-PCR analysis showed that concomitant with the onset of fruit colour development in strawberry, levels of expression of the βglucosidase 1 gene (FaBG1) increased rapidly. Furthermore, 15 mM histamine, an inhibitor of βglucosidase, significantly inhibited red-colour development in strawberry fruit. These results suggest that FaBG1 might play a role in strawberry fruit ripening. A combination of enzyme incubation assays with receptacle tissue and GC-MS analysis demonstrated that the red-colour in strawberry fruit was rich in abscisic acid (ABA)-glucosyl ester (ABA-GE) and that FaBG1 had high glycosyl hydrolase activity, which could promote the accumulation of free ABA via hydrolysis of ABA-GE. Using tobacco rattle virus (TRV)-induced gene silencing (VIGS), down-regulation of FaBG1 gene expression not only inhibited strawberry fruit ripening, but also led to a significant decline in ABA concentration in VIGS-treated fruit. In conclusion, these results provide physiological, biochemical, and molecular evidence to validate the hypothesis that strawberry βglucosidase 1 (FaBG1) has high glycosyl hydrolase activity and plays a role in fruit ripening via the rapid production of free ABA.
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Claims 7
Claim · model-written summary
FaBG1 expression increased rapidly with the onset of fruit colour development in strawberry.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Organ / stage
- fruit colour developmente0
- Gene
- FaBG1e0
- Outcome
- levels of expressione0
- Direction
- increased rapidlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“Real-time RT-PCR analysis showed that concomitant with the onset of fruit colour development in strawberry, levels of expression of the βglucosidase 1 gene (FaBG1) increased rapidly”
Claim · model-written summary
15 mM histamine, an inhibitor of beta-glucosidase, significantly inhibited red-colour development in strawberry fruit.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- 15 mM histaminee0
- Outcome
- red-colour developmente0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“15 mM histamine, an inhibitor of βglucosidase, significantly inhibited red-colour development in strawberry fruit”
Claim · model-written summary
Red-coloured strawberry fruit was rich in ABA-glucosyl ester (ABA-GE).
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- rich in abscisic acid (ABA)-glucosyl ester (ABA-GE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the red-colour in strawberry fruit was rich in abscisic acid (ABA)-glucosyl ester (ABA-GE)”
Claim · model-written summary
FaBG1 had high glycosyl hydrolase activity in enzyme incubation assays with strawberry receptacle tissue.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaBG1e1
- Outcome
- high glycosyl hydrolase activitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strawberry (Fragaria ananassa Duch.) fruit flesh”
e1“FaBG1 had high glycosyl hydrolase activity, which could promote the accumulation of free ABA via hydrolysis of ABA-GE”
Claim · model-written summary
FaBG1 could promote the accumulation of free ABA in strawberry fruit via hydrolysis of ABA-GE.
- Taxon
- strawberryFragaria ×ananassae0
- Gene
- FaBG1e1
- Compound
- ABA-GEe1
- Outcome
- accumulation of free ABAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strawberry (Fragaria ananassa Duch.) fruit flesh”
e1“FaBG1 had high glycosyl hydrolase activity, which could promote the accumulation of free ABA via hydrolysis of ABA-GE”
Claim · model-written summary
Down-regulation of FaBG1 expression by VIGS inhibited strawberry fruit ripening.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- down-regulation of FaBG1 gene expressione0
- Gene
- FaBG1e0
- Outcome
- strawberry fruit ripeninge0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down-regulation of FaBG1 gene expression not only inhibited strawberry fruit ripening”
Claim · model-written summary
VIGS of FaBG1 led to a significant decline in ABA concentration in treated strawberry fruit.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- VIGSe1
- Outcome
- ABA concentratione1
- Direction
- declinee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strawberry (Fragaria ananassa Duch.) fruit flesh”
e1“led to a significant decline in ABA concentration in VIGS-treated fruit”
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See all 22 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.