Abstract
Abstract Background Although panicle blast is more destructive to yield loss than leaf blast in rice, the cloned genes that function in panicle blast resistance are still very limited and the molecular mechanisms underlying panicle blast resistance remain largely unknown. Results In the present study, we have confirmed that the three Oxalate oxidase ( OXO ) genes, OsOXO2 , OsOXO3 and OsOXO4 from a blast-resistant cultivar BC10 function in panicle blast resistance in rice. The expression of OsOXO2 , OsOXO3 and OsOXO4 were induced by panicle blast inoculation. Subcellular localization analysis revealed that the three OXO proteins are all localized in the nucleus and cytoplasm. Simultaneous silencing of OsOXO2 , OsOXO3 and OsOXO4 decreased rice resistance to panicle blast, whereas the OsOXO2 , OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance. More H 2 O 2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants, while the silencing plants exhibited less H 2 O 2 and lower expression levels of PR genes compared to the control plants. Moreover, phytohormone treatment and the phytohormone signaling related gene expression analysis showed that panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways but suppression of SA signaling pathway. Conclusion OsOXO2 , OsOXO3 and OsOXO4 positively regulate panicle blast resistance in rice. The OXO genes could modulate the accumulation of H 2 O 2 and expression levels of PR gene in plants. Moreover, the OXO genes mediated panicle blast resistance could be regulated by ABA, SA and JA, and may be associated with the activation of JA and ABA signaling pathways but suppression of the SA signaling pathway.
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Claims 12
Claim · model-written summary
Panicle blast inoculation induced expression of the three OXO genes.
- Taxon
- rice
Oryza sativat0 - Treatment
- panicle blast inoculatione0
- Gene
- OsOXO2 , OsOXO3 and OsOXO4e0
- Outcome
- expression of OsOXO2 , OsOXO3 and OsOXO4e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of OsOXO2 , OsOXO3 and OsOXO4 were induced by panicle blast inoculation”
t0“Rice”
Claim · model-written summary
The three OXO proteins localized to nucleus and cytoplasm.
- Taxon
- riceOryza sativat0
- Gene
- OXO proteinse0
- Outcome
- localized in the nucleus and cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three OXO proteins are all localized in the nucleus and cytoplasm”
t0“Rice”
Claim · model-written summary
Simultaneous OXO gene silencing reduced rice panicle blast resistance.
- Taxon
- riceOryza sativae0
- Treatment
- Simultaneous silencing of OsOXO2 , OsOXO3 and OsOXO4e0
- Gene
- OsOXO2 , OsOXO3 and OsOXO4e0
- Outcome
- rice resistance to panicle blaste0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Simultaneous silencing of OsOXO2 , OsOXO3 and OsOXO4 decreased rice resistance to panicle blast”
t0“Rice”
Claim · model-written summary
OsOXO2 overexpression enhanced panicle blast resistance.
- Taxon
- riceOryza sativae0
- Treatment
- OsOXO2e0
- Gene
- OsOXO2e0
- Outcome
- panicle blast resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsOXO2 , OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance”
t0“Rice”
Claim · model-written summary
OsOXO3 overexpression enhanced panicle blast resistance.
- Taxon
- riceOryza sativae0
- Treatment
- OsOXO3e0
- Gene
- OsOXO3e0
- Outcome
- panicle blast resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsOXO2 , OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance”
t0“Rice”
Claim · model-written summary
OsOXO4 overexpression enhanced panicle blast resistance.
- Taxon
- riceOryza sativae0
- Treatment
- OsOXO4e0
- Gene
- OsOXO4e0
- Outcome
- panicle blast resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsOXO2 , OsOXO3 and OsOXO4 overexpression rice plants individually showed enhanced panicle blast resistance”
t0“Rice”
Claim · model-written summary
OXO overexpression increased H 2 O 2 relative to controls.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressing plantse0
- Outcome
- H 2 O 2e0
- Direction
- Moree0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More H 2 O 2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants”
t0“Rice”
Claim · model-written summary
OXO overexpression increased PR gene expression relative to controls.
- Taxon
- riceOryza sativat0
- Treatment
- overexpressing plantse0
- Outcome
- expression levels of PR genese0
- Direction
- highere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“More H 2 O 2 and higher expression levels of PR genes were observed in the overexpressing plants than in the control plants”
t0“Rice”
Claim · model-written summary
OXO silencing reduced H 2 O 2 relative to controls.
- Taxon
- riceOryza sativat0
- Treatment
- silencing plantse0
- Outcome
- H 2 O 2e0
- Direction
- lesse0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing plants exhibited less H 2 O 2 and lower expression levels of PR genes compared to the control plants”
t0“Rice”
Claim · model-written summary
OXO silencing reduced PR gene expression relative to controls.
- Taxon
- riceOryza sativat0
- Treatment
- silencing plantse0
- Outcome
- expression levels of PR genese0
- Direction
- lowere0
- Comparator
- control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing plants exhibited less H 2 O 2 and lower expression levels of PR genes compared to the control plants”
t0“Rice”
Claim · model-written summary
OXO-mediated resistance was associated with activation of JA and ABA signaling.
- Taxon
- riceOryza sativat0
- Gene
- OXO genese0
- Outcome
- panicle blast resistancee0
- Qualifier
- activation of JA and ABA signaling pathwayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways”
t0“Rice”
Claim · model-written summary
OXO-mediated resistance was associated with suppression of SA signaling.
- Taxon
- riceOryza sativat0
- Gene
- OXO genese1
- Outcome
- panicle blast resistancee1
- Qualifier
- suppression of SA signaling pathwaye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of SA signaling pathway”
t0“Rice”
e1“panicle blast resistance mediated by the three OXO genes was associated with the activation of JA and ABA signaling pathways”
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