Abstract
Iron (Fe) deficiency impairs photosynthetic efficiency, plant growth and biomass yield. This study aimed to reveal the role of nitric oxide (NO) in restoring Fe-homeostasis and oxidative status in Fe-deficient alfalfa. In alfalfa, a shortage of Fe negatively affected the efficiency of root andshoot length, leaf greenness, maximum quantum yield PSII (Fv/Fm), Fe, S, and Zn accumulation, as well as an increase in H2O2 accumulation. In contrast, in the presence of sodium nitroprusside (SNP), a NO donor, these negative effects of Fe deficiency were largely reversed. In response to the SNP, the expression of Fe transporters (IRT1, NRAMP1) and S transporter (SULTR1;2) genes increased in alfalfa. Additionally, the detection of NO generation using fluorescence microscope revealed that SNP treatment increased the level of NO signal, indicating that NO may act as regulatory signal in response to SNP in plants. Interestingly, the increase of antioxidant genes and their related enzymes (Fe-SOD, APX) in response to SNP treatment suggests that Fe-SOD and APX are key contributors to reducing ROS (H2O2) accumulation and oxidative stress in alfalfa. Furthermore, the elevation of Ascorbate-glutathione (AsA-GSH) pathway-related genes (GR and MDAR) Fe-deficiency with SNP implies that the presence of NO relates to enhanced antioxidant defense against Fe-deficiency stress.
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Claims 9
Claim · model-written summary
Fe deficiency reduced alfalfa root and shoot length, leaf greenness, and PSII maximum quantum yield.
- Taxon
- Alfalfa
Medicago sativat0 - Treatment
- a shortage of Fee0
- Outcome
- root andshoot length, leaf greenness, maximum quantum yield PSII (Fv/Fm)e0
- Direction
- negatively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“a shortage of Fe negatively affected the efficiency of root andshoot length, leaf greenness, maximum quantum yield PSII (Fv/Fm)”
Claim · model-written summary
Fe deficiency reduced Fe, S, and Zn accumulation in alfalfa.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- a shortage of Fee0
- Outcome
- Fe, S, and Zn accumulatione0
- Direction
- negatively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“a shortage of Fe negatively affected the efficiency of root andshoot length, leaf greenness, maximum quantum yield PSII (Fv/Fm), Fe, S, and Zn accumulation”
Claim · model-written summary
Fe deficiency increased H2O2 accumulation in alfalfa.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- a shortage of Fee0
- Outcome
- H2O2 accumulatione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“a shortage of Fe negatively affected the efficiency of root andshoot length, leaf greenness, maximum quantum yield PSII (Fv/Fm), Fe, S, and Zn accumulation, as well as an increase in H2O2 accumulation”
Claim · model-written summary
SNP largely reversed the reported effects of Fe deficiency in alfalfa.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- sodium nitroprusside (SNP)e0
- Outcome
- negative effects of Fe deficiencye0
- Direction
- reversede0
- Qualifier
- largelye0
- Comparator
- Fe deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“in the presence of sodium nitroprusside (SNP), a NO donor, these negative effects of Fe deficiency were largely reversed”
Claim · model-written summary
SNP increased expression of IRT1, NRAMP1, and SULTR1;2 in alfalfa.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- SNPe0
- Gene
- IRT1, NRAMP1e0
- Outcome
- expression of Fe transporters (IRT1, NRAMP1) and S transporter (SULTR1;2) genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“In response to the SNP, the expression of Fe transporters (IRT1, NRAMP1) and S transporter (SULTR1;2) genes increased in alfalfa”
Claim · model-written summary
SNP increased NO signal in alfalfa.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- SNP treatmente0
- Outcome
- level of NO signale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“SNP treatment increased the level of NO signal”
Claim · model-written summary
SNP increased antioxidant genes and related enzymes Fe-SOD and APX in alfalfa.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- SNP treatmente0
- Gene
- Fe-SOD, APXe0
- Outcome
- antioxidant genes and their related enzymes (Fe-SOD, APX)e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“increase of antioxidant genes and their related enzymes (Fe-SOD, APX) in response to SNP treatment”
Claim · model-written summary
The authors suggest Fe-SOD and APX contribute to reducing H2O2 accumulation and oxidative stress in alfalfa.
- Taxon
- alfalfaMedicago sativae0
- Gene
- Fe-SOD and APXe0
- Outcome
- ROS (H2O2) accumulation and oxidative stresse0
- Direction
- reducinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“Fe-SOD and APX are key contributors to reducing ROS (H2O2) accumulation and oxidative stress in alfalfa”
Claim · model-written summary
GR and MDAR pathway-related genes were elevated under Fe deficiency with SNP in alfalfa.
- Taxon
- AlfalfaMedicago sativat0
- Treatment
- Fe-deficiency with SNPe0
- Gene
- GR and MDARe0
- Outcome
- Ascorbate-glutathione (AsA-GSH) pathway-related genes (GR and MDAR)e0
- Direction
- elevatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Alfalfa”
e0“elevation of Ascorbate-glutathione (AsA-GSH) pathway-related genes (GR and MDAR) Fe-deficiency with SNP”
The same questions in other papers
iron atom · nutrient: content · Medicago sativa (alfalfa)Positive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- negative
- positive
1 paper more in this group
See all 5 papers →sodium nitroprusside · nutrient: content · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- positive
sodium nitroprusside · molecular: metabolites · Medicago sativa (alfalfa)Results agree
1 paper in the same direction as this paper
- positive
iron atom · physiology: photosynthesis · Medicago sativa (alfalfa)Results agree
2 papers in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.