Abstract
Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different soil and climatic zones of Ukraine. Methods. Serological, gas chromatography, field experiment and statistical. Results. The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12). The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control. Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia. Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown. Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties. The competitiveness of M. ciceri ND-64 was determined using the immunological method in the field experiment. It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation in the variant with inoculation of the strain M. ciceri ND-64 100 % of the nodules were formed by representatives of this serogroup. Conclusion. M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants, due to its virulence, competitiveness, nitrogen-fixing properties. According to the results of field experiments, it was shown that the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil. Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety. Therefore, M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality.
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Claims 14
Claim · model-written summary
Pre-sowing inoculation with M. ciceri ND-64 increased nitrogen-fixing nodules by 5% to 67% relative to the positive control in Steppe field trials.
- Taxon
- chickpea
Cicer arietinume0 - Material
- Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiate0
- Treatment
- pre-sowing bacterization of chickpea seedse0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie4symbiont
- Outcome
- nitrogen-fixing nodulese1
- Direction
- increasee1
- Value
- 5 % to 67 %e1
- Comparator
- positive controle2
- Setting
- Steppe zonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat”
e1“contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %”
e2“compared with the positive control (inoculation with the reference strain of M. ciceri H-12)”
e3“upon growing in field experiments in the Steppe zone”
e4“The use of a new strain of M. ciceri ND-64”
Claim · model-written summary
Pre-sowing inoculation with M. ciceri ND-64 increased nodule mass by 10% to 67% relative to the positive control.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- pre-sowing bacterization of chickpea seedse0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie4symbiont
- Outcome
- mass of nodulese3
- Direction
- increasee3
- Value
- 10 % to 67 %e3
- Comparator
- positive controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat”
e2“compared with the positive control (inoculation with the reference strain of M. ciceri H-12)”
e3“an increase in the mass of nodules by 10 % to 67 %”
e4“The use of a new strain of M. ciceri ND-64”
Claim · model-written summary
Pre-sowing inoculation with M. ciceri ND-64 increased nitrogenase activity by 26% to 150% relative to the positive control.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- pre-sowing bacterization of chickpea seedse0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie5symbiont
- Outcome
- nitrogenase activitye4
- Direction
- increasee1
- Value
- 26 % to 150 %e4
- Comparator
- positive controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat”
e1“contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %”
e2“compared with the positive control (inoculation with the reference strain of M. ciceri H-12)”
e4“nitrogenase activity 26 % to 150 %”
e5“The use of a new strain of M. ciceri ND-64”
Claim · model-written summary
Pre-sowing inoculation with M. ciceri ND-64 increased chickpea yield by 4% to 18% relative to the positive control.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- pre-sowing bacterization of chickpea seedse0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie3symbiont
- Outcome
- yield of chickpeae2
- Direction
- increasinge2
- Value
- 4 % to 18 %e5
- Comparator
- positive controle5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat”
e5“by 4 % to 18 % compared to the positive control”
e2“positive effect on increasing the yield of chickpea”
e3“The use of a new strain of M. ciceri ND-64”
Claim · model-written summary
In the Polissia zone, inoculation with the new strain gave 57% to 89% more nodules than the positive control.
- Taxon
- chickpeaCicer arietinume6
- Material
- Skarb, Triumf and Pamiate6
- Treatment
- Inoculation with a new straine6
- Outcome
- number of nodulese6
- Direction
- highere6
- Value
- 57 % to 89 %e6
- Comparator
- positive controle2
- Setting
- Polissia zonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“compared with the positive control (inoculation with the reference strain of M. ciceri H-12)”
e6“Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %)”
e3“cultivation in the Polissia zone”
Claim · model-written summary
In the Polissia zone, inoculation with the new strain gave nitrogenase activity 2 to 4 times that of the positive control.
- Taxon
- chickpeaCicer arietinume6
- Treatment
- Inoculation with a new straine6
- Outcome
- nitrogenase activitye4
- Direction
- higherinferrede6
- Value
- 2–4 timese4
- Comparator
- positive controle2
- Setting
- Polissia zonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“compared with the positive control (inoculation with the reference strain of M. ciceri H-12)”
e6“Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %)”
e4“their nitrogenase activity (2–4 times)”
e3“cultivation in the Polissia zone”
Claim · model-written summary
In the Polissia zone, inoculation with the new strain gave nodule mass 2.2 to 2.9 times that of the positive control.
- Taxon
- chickpeaCicer arietinume6
- Treatment
- Inoculation with a new straine6
- Outcome
- mass of nodulese4
- Direction
- increaseede4
- Value
- 2.2–2.9 timese4
- Comparator
- positive controle2
- Setting
- Polissia zonee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“compared with the positive control (inoculation with the reference strain of M. ciceri H-12)”
e6“Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %)”
e4“increaseed mass of nodules (2.2–2.9 times)”
e3“cultivation in the Polissia zone”
Claim · model-written summary
Inoculation with M. ciceri ND-64 increased structural parameters of chickpea yield.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- structural parameters of yielde0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown”
Claim · model-written summary
Inoculation with M. ciceri ND-64 increased photosynthetic activity and grain protein content.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- photosynthetic activitye0
- Direction
- increasee0
- Qualifier
- protein content in graine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown”
Claim · model-written summary
Inoculation with M. ciceri ND-64 gave the highest gain in chickpea yield.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- gain in chickpea yielde0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown”
Claim · model-written summary
M. ciceri ND-64 formed 100% of the nodules in the variant where it was applied.
- Taxon
- chickpeaCicer arietinume1
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- nodulese0
- Value
- 100 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“100 % of nodules have been formed by the representatives of M. ciceri ND-64”
e1“M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants”
Claim · model-written summary
An immunological method detected serological relatedness among M. ciceri ND-64 and related strains.
- Taxon
- chickpeaCicer arietinume1
- Outcome
- serological relatednesse0
- Comparator
- M. ciceri H-101, Mesorhizobium sp. ND-601e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601”
e1“M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants”
Claim · model-written summary
Seed treatment with M. ciceri ND-64 gave symbiotic parameters exceeding those of the reference strain M. ciceri H-12.
- Taxon
- chickpeaCicer arietinume1
- Treatment
- suspension of M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- Symbiotic parameterse0
- Direction
- exceede0
- Comparator
- reference strain of M. ciceri H-12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12”
e1“M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants”
Claim · model-written summary
The authors recommend using M. ciceri ND-64 as a bioagent in a microbial preparation for chickpea inoculation.
- Taxon
- chickpeaCicer arietinume0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- used as a bioagent of a microbial preparatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties”
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