Abstract
Objective. Study the competitiveness, complementarity and symbiotic activity of a new strain of chickpea nodule bacteria Mesorhizobium ciceri ND-64, study the influence of seed inoculation on the productivity and yield of chickpea varieties of Ukrainian selection under their cultivation in different soil and climatic zones of Ukraine. Methods. Serological, gas chromatography, field experiment and statistical. Results. The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12). The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control. Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia. Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown. Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties. The competitiveness of M. ciceri ND-64 was determined using the immunological method in the field experiment. It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation in the variant with inoculation of the strain M. ciceri ND-64 100 % of the nodules were formed by representatives of this serogroup. Conclusion. M. ciceri ND-64 strain is an effective microsymbiont of chickpea plants, due to its virulence, competitiveness, nitrogen-fixing properties. According to the results of field experiments, it was shown that the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil. Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety. Therefore, M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality.
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Claims 16
Claim · model-written summary
ND-64 inoculation increased nitrogen-fixing nodules by 5 % to 67 % versus the reference strain in the Steppe.
- Taxon
- chickpea
Cicer arietinume0 - Treatment
- pre-sowing bacterizatione0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- nitrogen-fixing nodulese0
- Direction
- increasee0
- Value
- 5 % to 67 %e0
- Comparator
- inoculation with the reference strain of M. ciceri H-12e0
- Setting
- Steppe zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12).”
Claim · model-written summary
ND-64 inoculation increased mass of nodules by 10 % to 67 % versus the reference strain in the Steppe.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- pre-sowing bacterizatione0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- mass of nodulese0
- Direction
- increasee0
- Value
- 10 % to 67 %e0
- Comparator
- inoculation with the reference strain of M. ciceri H-12e0
- Setting
- Steppe zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12).”
Claim · model-written summary
ND-64 inoculation increased nitrogenase activity by 26 % to 150 % versus the reference strain in the Steppe.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- pre-sowing bacterizatione0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- nitrogenase activitye0
- Direction
- increasee0
- Value
- 26 % to 150 %e0
- Comparator
- inoculation with the reference strain of M. ciceri H-12e0
- Setting
- Steppe zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of a new strain of M. ciceri ND-64 for pre-sowing bacterization of chickpea seeds of varieties Skarb, Admiral, Odysei, Budzhak, Triumf and Pamiat upon growing in field experiments in the Steppe zone against the background of the local population of nodule bacteria contributes to a significant increase in nitrogen-fixing nodules by 5 % to 67 %, an increase in the mass of nodules by 10 % to 67 % and nitrogenase activity 26 % to 150 % compared with the positive control (inoculation with the reference strain of M. ciceri H-12).”
Claim · model-written summary
ND-64 inoculation increased chickpea yield by 4% to 18% versus the reference strain in the Steppe.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- pre-sowing bacterizatione0
- Interactor
- M. ciceri ND- 64Mesorhizobium cicerie0symbiont
- Outcome
- yield of chickpeae0
- Direction
- increasinge0
- Value
- 4 % to 18 %e0
- Comparator
- positive controle0
- Setting
- Steppe zone of Ukrainee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The use of M. ciceri ND- 64 for pre-sowing bacterization of chickpea seeds under cultivation in the Steppe zone of Ukraine also had a positive effect on increasing the yield of chickpea — by 4 % to 18 % compared to the positive control.”
Claim · model-written summary
ND-64 inoculation increased number of nodules by 57 % to 89 % in Polissia versus the positive control.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- Inoculation with a new straine0
- Outcome
- number of nodulese0
- Direction
- highere0
- Value
- 57 % to 89 %e0
- Comparator
- positive controle0
- Setting
- Polissia zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia.”
Claim · model-written summary
ND-64 inoculation increased mass of nodules by 2.2–2.9 times in Polissia versus the positive control.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- Inoculation with a new straine0
- Outcome
- mass of nodulese0
- Direction
- increaseede0
- Value
- 2.2–2.9 timese0
- Comparator
- positive controle0
- Setting
- Polissia zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia.”
Claim · model-written summary
ND-64 inoculation increased nitrogenase activity by 2–4 times in Polissia versus the positive control.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- Inoculation with a new straine0
- Outcome
- nitrogenase activitye0
- Direction
- increaseede0
- Value
- 2–4 timese0
- Comparator
- positive controle0
- Setting
- Polissia zonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation with a new strain of chickpea seeds of varieties Skarb, Triumf and Pamiat contributed to the formation of higher number of nodules (by 57 % to 89 %), increaseed mass of nodules (2.2–2.9 times) and their nitrogenase activity (2–4 times) compared with the positive control upon cultivation in the Polissia zone on the fields where there is no population of chickpea rhizobia.”
Claim · model-written summary
ND-64 inoculation increased structural parameters of yield under Polissia conditions.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- structural parameters of yielde0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown.”
Claim · model-written summary
ND-64 inoculation increased chickpea yield under Polissia conditions.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- chickpea yielde0
- Direction
- highest gaine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown.”
Claim · model-written summary
ND-64 inoculation increased photosynthetic activity under Polissia conditions.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- photosynthetic activitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown.”
Claim · model-written summary
ND-64 inoculation increased protein content in grain under Polissia conditions.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- inoculation with M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- protein content in graine0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under these conditions, an increase in structural parameters of yield, the highest gain in chickpea yield, increase in photosynthetic activity and protein content in grain upon inoculation with M. ciceri ND-64 was shown.”
Claim · model-written summary
Antiserum detected serological relatedness among the listed Mesorhizobium strains isolated from chickpea nodules.
- Taxon
- chickpeaCicer arietinume0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- serological relatednesse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Obtaining a specific antisera by immunization of rabbits allowed to detect serological relatedness of M. ciceri ND-64, M. ciceri H-101, Mesorhizobium sp. ND-601 and M. ciceri ND-64, isolated from chickpea nodules of Skarb and Pamiat varieties.”
Claim · model-written summary
All nodules in the ND-64 inoculation variant were formed by representatives of its serogroup.
- Taxon
- chickpeaCicer arietinumt0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- nodules have been formed by the representatives of M. ciceri ND-64e0
- Value
- 100 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It was found that 100 % of nodules have been formed by the representatives of M. ciceri ND-64 in the variant where this serological group was applied for inoculation”
t0“CHICKPEA”
Claim · model-written summary
ND-64 formed an effective symbiosis across the tested varieties with or without local rhizobia.
- Taxon
- chickpeaCicer arietinume0
- Interactor
- M. cicerіe0symbiont
- Outcome
- effective symbiosise0
- Qualifier
- both against the active local population of rhizobia and upon the absence of M. cicerі population in the soile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the new strain, complementary to all studied varieties differing in seed size and shape, type of bush and average height, forms an effective symbiosis for growing chickpea plants both against the active local population of rhizobia and upon the absence of M. cicerі population in the soil.”
Claim · model-written summary
ND-64 seed treatment exceeded reference and variety-derived strains in symbiotic parameters.
- Taxon
- chickpeaCicer arietinumt0
- Treatment
- seed treatment with suspension of M. ciceri ND-64e0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- Symbiotic parameterse0
- Direction
- exceede0
- Comparator
- reference strain of M. ciceri H-12 and strains isolated from the nodules of each study varietye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Symbiotic parameters upon seed treatment with suspension of M. ciceri ND-64 exceed these values in the variants with inoculation using the reference strain of M. ciceri H-12 and strains isolated from the nodules of each study variety.”
t0“CHICKPEA”
Claim · model-written summary
The authors recommend ND-64 as a chickpea seed inoculant to improve symbiosis, productivity and seed quality.
- Taxon
- chickpeaCicer arietinume0
- Interactor
- M. ciceri ND-64Mesorhizobium cicerie0symbiont
- Outcome
- used as a bioagent of a microbial preparation for inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“M. ciceri ND-64 can be used as a bioagent of a microbial preparation for inoculation of chickpea of different varieties in order to form an effective bean-rhizobial symbiosis, increase the productivity of this culture and improve seed quality.”
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