Abstract
Rose plants are one of the most important horticultural crops, whose commercial value mainly depends on long-distance transportation, and wounding and ethylene are the main factors leading to their quality decline and accelerated senescence in the process. However, underlying molecular mechanisms of crosstalk between wounding and ethylene in the regulation of flower senescence remain poorly understood. In relation to this, transcriptome analysis was performed on rose flowers subjected to various treatments, including control, wounding, ethylene, and wounding- and ethylene- (EW) dual treatment. A large number of differentially expressed genes (DEGs) were identified, ranging from 2,442 between the ethylene- and control-treated groups to 4,055 between the EW- and control-treated groups. Using weighted gene co-expression network analysis (WGCNA), we identified a hub gene RhWRKY33 (rchiobhmchr5g0071811), accumulated in the nucleus, where it may function as a transcription factor. Moreover, quantitative reverse transcription PCR (RT-qPCR) results showed that the expression of RhWRKY33 was higher in the wounding-, ethylene, and EW-treated petals than in the control-treated petals. We also functionally characterized the RhWRKY33 gene through virus-induced gene silencing (VIGS). The silencing of RhWRKY33 significantly delayed the senescence process in the different treatments (control, wounding, ethylene, and EW). Meanwhile, we found that the effect of RhWRKY33 -silenced petals under ethylene and EW dual-treatment were stronger than those under wounding treatment in delaying the petal senescence process, implying that RhWRKY33 is closely involved with ethylene and wounding mediated petal senescence. Overall, the results indicate that RhWRKY33 positively regulates the onset of floral senescence mediated by both ethylene and wounding signaling, but relies heavily on ethylene signaling.
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Claims 8
Claim · model-written summary
Transcriptome analysis identified 2,442 DEGs between ethylene and control.
Verbatim
e0“A large number of differentially expressed genes (DEGs) were identified, ranging from 2,442 between the ethylene- and control-treated groups to 4,055 between the EW- and control-treated groups”
t0“Rose Plants”
Claim · model-written summary
Transcriptome analysis identified 4,055 DEGs between EW and control.
Verbatim
e0“A large number of differentially expressed genes (DEGs) were identified, ranging from 2,442 between the ethylene- and control-treated groups to 4,055 between the EW- and control-treated groups”
t0“Rose Plants”
Claim · model-written summary
Network analysis identified RhWRKY33 as a hub gene.
Verbatim
e0“we identified a hub gene RhWRKY33 (rchiobhmchr5g0071811), accumulated in the nucleus, where it may function as a transcription factor”
t0“Rose Plants”
Claim · model-written summary
RhWRKY33 accumulated in the nucleus.
Verbatim
e0“RhWRKY33 (rchiobhmchr5g0071811), accumulated in the nucleus”
t0“Rose Plants”
Claim · model-written summary
RhWRKY33 expression was higher in wounding, ethylene and combined treatments than controls.
Verbatim
e0“expression of RhWRKY33 was higher in the wounding-, ethylene, and EW-treated petals than in the control-treated petals”
t0“Rose Plants”
Claim · model-written summary
RhWRKY33 silencing significantly delayed senescence across treatments.
- Taxon
- Rose Plantst0
- Treatment
- silencing of RhWRKY33e0
- Gene
- RhWRKY33e0
- Outcome
- senescence processe0
- Direction
- delayede0
Not reportedComparator
Verbatim
e0“silencing of RhWRKY33 significantly delayed the senescence process in the different treatments (control, wounding, ethylene, and EW)”
t0“Rose Plants”
Claim · model-written summary
The silencing delay under ethylene and combined treatment was stronger than under wounding.
Verbatim
e0“effect of RhWRKY33 -silenced petals under ethylene and EW dual-treatment were stronger than those under wounding treatment in delaying the petal senescence process”
t0“Rose Plants”
Claim · model-written summary
The authors infer RhWRKY33 promotes floral senescence through ethylene and wounding signalling, mainly ethylene.
Verbatim
e0“RhWRKY33 positively regulates the onset of floral senescence mediated by both ethylene and wounding signaling, but relies heavily on ethylene signaling”
t0“Rose Plants”
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