Abstract
Abstract Drought is a critical environmental factor which constrains plant survival and growth. Genetic engineering provides a credible strategy to improve drought tolerance of plants. Here, we generated transgenic poplar lines expressing the isopentenyl transferase gene ( IPT ) under the driver of PtRD26 promoter ( PtRD26 pro ‐IPT ). PtRD26 is a senescence and drought‐inducible NAC transcription factor. PtRD26 pro ‐IPT plants displayed multiple phenotypes, including improved growth and drought tolerance. Transcriptome analysis revealed that auxin biosynthesis pathway was activated in the PtRD26 pro ‐IPT plants, leading to an increase in auxin contents. Biochemical analysis revealed that ARABIDOPSIS RESPONSE REGULATOR10 ( PtARR10 ), one of the type‐B ARR transcription factors in the cytokinin pathway, was induced in PtRD26 pro ‐IPT plants and directly regulated the transcripts of YUCCA4 ( PtYUC4 ) and YUCCA5 ( PtYUC5 ), two enzymes in the auxin biosynthesis pathway. Overexpression of PtYUC4 enhanced drought tolerance, while simultaneous silencing of PtYUC4/5 evidently attenuated the drought tolerance of PtRD26 pro ‐IPT plants. Intriguingly, PtYUC4/5 displayed a conserved thioredoxin reductase activity that is required for drought tolerance by deterring reactive oxygen species accumulation. Our work reveals the molecular basis of cytokinin and auxin interactions in response to environmental stresses, and shed light on the improvement of drought tolerance without a growth penalty in trees by molecular breeding.
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Claims 10
Claim · model-written summary
PtRD26pro-IPT poplar plants displayed improved growth.
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtRD26 pro ‐IPT plants displayed multiple phenotypes, including improved growth and drought tolerance”
e1“transgenic poplar lines”
Claim · model-written summary
PtRD26pro-IPT poplar plants displayed improved drought tolerance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtRD26 pro ‐IPT plants displayed multiple phenotypes, including improved growth and drought tolerance”
e1“transgenic poplar lines”
Claim · model-written summary
The auxin biosynthesis pathway was activated in PtRD26pro-IPT poplar.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin biosynthesis pathway was activated in the PtRD26 pro ‐IPT plants”
e1“transgenic poplar lines”
Claim · model-written summary
Auxin content increased in PtRD26pro-IPT poplar.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin biosynthesis pathway was activated in the PtRD26 pro ‐IPT plants, leading to an increase in auxin contents”
e1“transgenic poplar lines”
Claim · model-written summary
PtARR10 was induced in PtRD26pro-IPT plants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtARR10 ), one of the type‐B ARR transcription factors in the cytokinin pathway, was induced in PtRD26 pro ‐IPT plants”
e1“transgenic poplar lines”
Claim · model-written summary
PtARR10 directly regulated PtYUC4 and PtYUC5 transcripts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“directly regulated the transcripts of YUCCA4 ( PtYUC4 ) and YUCCA5 ( PtYUC5 )”
e1“ARABIDOPSIS RESPONSE REGULATOR10 ( PtARR10 )”
e2“transgenic poplar lines”
Claim · model-written summary
Overexpression of PtYUC4 enhanced drought tolerance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PtYUC4 enhanced drought tolerance”
e1“transgenic poplar lines”
Claim · model-written summary
Simultaneous silencing of PtYUC4/5 attenuated drought tolerance of PtRD26pro-IPT plants.
- Material
- PtRD26 pro ‐IPT plantse0
- Treatment
- simultaneous silencing of PtYUC4/5e0
- Gene
- PtYUC4/5e0
- Outcome
- drought tolerancee0
- Direction
- attenuatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“simultaneous silencing of PtYUC4/5 evidently attenuated the drought tolerance of PtRD26 pro ‐IPT plants”
e1“transgenic poplar lines”
Claim · model-written summary
PtYUC4/5 displayed conserved thioredoxin reductase activity.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtYUC4/5 displayed a conserved thioredoxin reductase activity”
e1“transgenic poplar lines”
Claim · model-written summary
PtYUC4/5 thioredoxin reductase activity was required for drought tolerance through reduced reactive oxygen species accumulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtYUC4/5 displayed a conserved thioredoxin reductase activity that is required for drought tolerance by deterring reactive oxygen species accumulation”
e1“transgenic poplar lines”
The same questions in other papers
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