Abstract
The plant cuticle, composed of cutin and wax, is crucial for reducing non-stomatal water loss and enhancing drought tolerance. CYP86A genes are key regulators of cutin and wax biosynthesis, yet the mechanisms controlling their expression remain unclear. Here, we identified PtrC2H2.2-6, a C2H2-type transcription factor that is downregulated at both transcriptional and protein levels under water stress in poplar. Through bioinformatics and biochemical analyses, we demonstrated that PtrC2H2.2-6 interacts with PtrPPK1 to regulate drought tolerance by modulating two critical cuticle biosynthesis genes, PtrCYP86A7 and PtrCYP86A8. PtrC2H2.2-6 negatively regulates cutin and wax biosynthesis by binding to the CACT motif in the promoters of PtrCYP86A7 and PtrCYP86A8, thereby suppressing their expression. Conversely, PtrPPK1 interacts with PtrC2H2.2-6, phosphorylates it, and promotes its degradation, thereby relieving its repression of PtrCYP86A7/8. Functional validation revealed that RNAi-mediated silencing of PtrC2H2.2-6 enhanced wax accumulation and drought tolerance, while overexpression of PtrC2H2.2-6 reduced wax accumulation and drought resistance. Furthermore, overexpression of PtrCYP86A7 or PtrCYP86A8 increased wax accumulation, enhanced the water retention capacity of the leaf surface, and improved drought resistance. Collectively, the PtrPPK1-PtrC2H2.2-6-PtrCYP86A7/A8 module is a critical regulatory mechanism for drought tolerance in poplar, offering potential targets for breeding drought-resistant forest trees.
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Claims 9
Claim · model-written summary
PtrC2H2.2-6 was downregulated at transcriptional and protein levels during water stress.
- Treatment
- water stresse0
- Gene
- PtrC2H2.2-6e0
- Outcome
- transcriptional and protein levelse0
- Direction
- downregulatede0
PopulusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrC2H2.2-6, a C2H2-type transcription factor that is downregulated at both transcriptional and protein levels under water stress in poplar”
Claim · model-written summary
PtrC2H2.2-6 interacted with PtrPPK1 to regulate drought tolerance through PtrCYP86A7 and PtrCYP86A8.
- Gene
- PtrC2H2.2-6e0
- Outcome
- drought tolerancee0
- Qualifier
- by modulating two critical cuticle biosynthesis genes, PtrCYP86A7 and PtrCYP86A8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrC2H2.2-6 interacts with PtrPPK1 to regulate drought tolerance by modulating two critical cuticle biosynthesis genes, PtrCYP86A7 and PtrCYP86A8”
t0“Poplar”
Claim · model-written summary
PtrC2H2.2-6 suppressed PtrCYP86A7 and PtrCYP86A8 expression through promoter binding.
- Gene
- PtrC2H2.2-6e0
- Outcome
- their expressione0
- Direction
- suppressinge0
- Qualifier
- binding to the CACT motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrC2H2.2-6 negatively regulates cutin and wax biosynthesis by binding to the CACT motif in the promoters of PtrCYP86A7 and PtrCYP86A8, thereby suppressing their expression”
t0“Poplar”
Claim · model-written summary
PtrC2H2.2-6 negatively regulated cutin and wax biosynthesis.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrC2H2.2-6 negatively regulates cutin and wax biosynthesis”
t0“Poplar”
Claim · model-written summary
PtrPPK1 phosphorylated PtrC2H2.2-6 and promoted its degradation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PtrPPK1 interacts with PtrC2H2.2-6, phosphorylates it, and promotes its degradation”
t0“Poplar”
Claim · model-written summary
Silencing PtrC2H2.2-6 enhanced wax accumulation and drought tolerance.
- Treatment
- RNAi-mediated silencing of PtrC2H2.2-6e0
- Gene
- PtrC2H2.2-6e0
- Outcome
- wax accumulation and drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNAi-mediated silencing of PtrC2H2.2-6 enhanced wax accumulation and drought tolerance”
t0“Poplar”
Claim · model-written summary
Overexpression of PtrC2H2.2-6 reduced wax accumulation and drought resistance.
- Treatment
- overexpression of PtrC2H2.2-6e0
- Gene
- PtrC2H2.2-6e0
- Outcome
- wax accumulation and drought resistancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PtrC2H2.2-6 reduced wax accumulation and drought resistance”
t0“Poplar”
Claim · model-written summary
PtrCYP86A7 overexpression increased wax accumulation, leaf water retention, and drought resistance.
- Treatment
- overexpression of PtrCYP86A7e0
- Gene
- PtrCYP86A7e0
- Outcome
- wax accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PtrCYP86A7 or PtrCYP86A8 increased wax accumulation, enhanced the water retention capacity of the leaf surface, and improved drought resistance”
t0“Poplar”
Claim · model-written summary
PtrCYP86A8 overexpression increased wax accumulation and improved drought resistance.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of PtrCYP86A7 or PtrCYP86A8 increased wax accumulation, enhanced the water retention capacity of the leaf surface, and improved drought resistance”
t0“Poplar”
The same questions in other papers
loss of function · tolerance: abiotic · Populus (poplar)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.