Abstract
Valsa mali is the main pathogenic fungus that causes the apple Valsa canker, a destructive disease severely threatening apple production in the world. However, the underlying key components involved in resistance against V. mali in apple trees remain largely unexplored. Here, we isolated and functionally characterized a G-type lectin S-receptor-like protein kinase MdSRLK3 from the cultivar Royal Gala derivative line GL-3. qRT-PCR showed that the relative expression of MdSRLK3 in apple branches reached its highest level at 24 h post V. mali inoculation, which was 13.42 times higher than without inoculation. Transient overexpression of MdSRLK3 enhanced apple resistance against V. mali, while transient silencing of MdSRLK3 reduced its resistance against the pathogen. More importantly, stable silencing of MdSRLK3 resulted in reduced resistance against this fungus. Furthermore, we demonstrated that MdSRLK3 positively regulated apple resistance by affecting the Ca 2+ signaling pathway, and the regulation was also related to the H 2 O 2 and callose signaling pathways. Overall, our data reveal that MdSRLK3 is a positive regulator of apple immunity.
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Claims 7
Claim · model-written summary
MdSRLK3 expression in apple branches peaked 24 hours after V. mali inoculation, at 13.42 times the uninoculated level.
- Taxon
- apple
Malus domesticae0 - Organ / stage
- branchese0
- Treatment
- V. mali inoculatione0
- Interactor
- V. maliCytospora malie0pathogen
- Gene
- MdSRLK3e0
- Outcome
- relative expression of MdSRLK3e0
- Direction
- higheste0
- Value
- 13.42 timese0
- Comparator
- without inoculatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the relative expression of MdSRLK3 in apple branches reached its highest level at 24 h post V. mali inoculation, which was 13.42 times higher than without inoculation”
Claim · model-written summary
Transient MdSRLK3 overexpression increased apple resistance to V. mali.
- Taxon
- appleMalus domesticae0
- Treatment
- Transient overexpression of MdSRLK3e0
- Interactor
- V. maliCytospora malie0pathogen
- Gene
- MdSRLK3e0
- Outcome
- apple resistance against V. malie0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient overexpression of MdSRLK3 enhanced apple resistance against V. mali”
Claim · model-written summary
Transient MdSRLK3 silencing reduced apple resistance to the pathogen.
- Taxon
- appleMalus domesticat0
- Treatment
- transient silencing of MdSRLK3e0
- Interactor
- Valsa maliCytospora malit0pathogen
- Gene
- MdSRLK3e0
- Outcome
- resistance against the pathogene0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transient silencing of MdSRLK3 reduced its resistance against the pathogen”
t0“The Apple Receptor-Like Kinase MdSRLK3 Positively Regulates Resistance Against Pathogenic Fungus Valsa mali”
Claim · model-written summary
Stable MdSRLK3 silencing reduced apple resistance to the fungus.
- Taxon
- appleMalus domesticat0
- Treatment
- stable silencing of MdSRLK3e0
- Interactor
- Valsa maliCytospora malit0pathogen
- Gene
- MdSRLK3e0
- Outcome
- resistance against this funguse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stable silencing of MdSRLK3 resulted in reduced resistance against this fungus”
t0“The Apple Receptor-Like Kinase MdSRLK3 Positively Regulates Resistance Against Pathogenic Fungus Valsa mali”
Claim · model-written summary
MdSRLK3 positively regulated apple resistance by affecting calcium signaling.
- Taxon
- appleMalus domesticae0
- Gene
- MdSRLK3e0
- Outcome
- apple resistancee0
- Direction
- positively regulatede0
- Qualifier
- Ca 2+ signaling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdSRLK3 positively regulated apple resistance by affecting the Ca 2+ signaling pathway”
Claim · model-written summary
MdSRLK3 regulation of resistance was related to hydrogen peroxide signaling.
- Taxon
- appleMalus domesticat0
- Gene
- MdSRLK3t0
- Outcome
- regulatione0
- Qualifier
- H 2 O 2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regulation was also related to the H 2 O 2 and callose signaling pathways”
t0“The Apple Receptor-Like Kinase MdSRLK3 Positively Regulates Resistance Against Pathogenic Fungus Valsa mali”
Claim · model-written summary
MdSRLK3 regulation of resistance was related to callose signaling.
- Taxon
- appleMalus domesticat0
- Gene
- MdSRLK3t0
- Outcome
- regulatione0
- Qualifier
- callose signaling pathwayse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the regulation was also related to the H 2 O 2 and callose signaling pathways”
t0“The Apple Receptor-Like Kinase MdSRLK3 Positively Regulates Resistance Against Pathogenic Fungus Valsa mali”
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See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.