Abstract
Mineral nutrients, such as manganese (Mn) and iron (Fe), play essential roles in many biological processes in plants but their over-enrichment is harmful for the metabolism. Metal tolerance proteins (MTPs) are involved in cellular Mn and Fe homeostasis. However, the transporter responsible for the transport of Mn in wheat is unknown. In our study, TuMTP8, a Mn-CDF transporter from diploid wheat (Triticum urartu), was identified. Expression of TuMTP8 in yeast strains of Δccc1 and Δsmf1 and Arabidopsis conferred tolerance to elevated Mn and Fe, but not to other metals (zinc, cobalt, copper, nickel, or cadmium). Compared with TuVIT1 (vacuole Fe transporter), TuMTP8 shows a significantly higher proportion in Mn transport and a smaller proportion in Fe transport. The transient analysis in tobacco epidermal cells revealed that TuMTP8 localizes to vacuolar membrane. The highest transcript levels of TuMTP8 were in the sheath of the oldest leaf and the awn, suggesting that TuMTP8 sequesters excess Mn into the vacuole in these organs, away from more sensitive tissues. These findings indicate that TuMTP8, a tonoplast-localized Mn/Fe transporter, functions as a primary balancer to regulate Mn distribution in T. urartu under elevated Mn conditions and participates in the intracellular transport and storage of excess Mn as a detoxification mechanism, thereby conferring Mn tolerance.
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Claims 9
Claim · model-written summary
TuMTP8 was identified as a Mn-CDF transporter from diploid wheat.
- Taxon
- Triticum urartu
Triticum urartue0 - Gene
- TuMTP8e0
- Outcome
- Mn-CDF transportere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TuMTP8, a Mn-CDF transporter from diploid wheat (Triticum urartu), was identified”
Claim · model-written summary
TuMTP8 expression conferred tolerance to elevated Mn and Fe in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Expression of TuMTP8e0
- Gene
- TuMTP8e0
- Outcome
- tolerance to elevated Mn and Fee0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TuMTP8 in yeast strains of Δccc1 and Δsmf1 and Arabidopsis conferred tolerance to elevated Mn and Fe, but not to other metals (zinc, cobalt, copper, nickel, or cadmium)”
Claim · model-written summary
TuMTP8 expression did not confer tolerance to other tested metals in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Expression of TuMTP8e0
- Gene
- TuMTP8e0
- Outcome
- other metals (zinc, cobalt, copper, nickel, or cadmium)e0
- Direction
- note0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of TuMTP8 in yeast strains of Δccc1 and Δsmf1 and Arabidopsis conferred tolerance to elevated Mn and Fe, but not to other metals (zinc, cobalt, copper, nickel, or cadmium)”
Claim · model-written summary
TuMTP8 had a higher proportion of Mn transport and a smaller proportion of Fe transport than TuVIT1.
- Taxon
- Triticum urartuTriticum urartut0
- Gene
- TuMTP8e0
- Outcome
- proportion in Mn transporte0
- Direction
- highere0
- Comparator
- TuVIT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with TuVIT1 (vacuole Fe transporter), TuMTP8 shows a significantly higher proportion in Mn transport and a smaller proportion in Fe transport”
t0“Triticum urartu”
Claim · model-written summary
TuMTP8 had a smaller proportion of Fe transport than TuVIT1.
- Taxon
- Triticum urartuTriticum urartut0
- Gene
- TuMTP8e0
- Outcome
- proportion in Fe transporte0
- Direction
- smallere0
- Comparator
- TuVIT1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared with TuVIT1 (vacuole Fe transporter), TuMTP8 shows a significantly higher proportion in Mn transport and a smaller proportion in Fe transport”
t0“Triticum urartu”
Claim · model-written summary
TuMTP8 localized to the vacuolar membrane in tobacco epidermal cells.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- epidermal cellse0
- Gene
- TuMTP8e0
- Outcome
- localizes to vacuolar membranee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transient analysis in tobacco epidermal cells revealed that TuMTP8 localizes to vacuolar membrane”
Claim · model-written summary
TuMTP8 transcript levels were highest in the sheath of the oldest leaf and awn.
- Taxon
- Triticum urartuTriticum urartut0
- Organ / stage
- sheath of the oldest leaf and the awne0
- Gene
- TuMTP8e0
- Outcome
- transcript levelse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest transcript levels of TuMTP8 were in the sheath of the oldest leaf and the awn”
t0“Triticum urartu”
Claim · model-written summary
The authors infer that TuMTP8 sequesters excess Mn in vacuoles of these organs.
- Taxon
- Triticum urartuTriticum urartut0
- Gene
- TuMTP8e0
- Outcome
- sequesters excess Mn into the vacuolee0
- Qualifier
- away from more sensitive tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that TuMTP8 sequesters excess Mn into the vacuole in these organs, away from more sensitive tissues”
t0“Triticum urartu”
Claim · model-written summary
TuMTP8 is interpreted as a primary regulator of Mn distribution and excess Mn storage under elevated Mn.
- Taxon
- T. urartuTriticum urartue0
- Gene
- TuMTP8e0
- Outcome
- Mn distributione0
- Qualifier
- under elevated Mn conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TuMTP8, a tonoplast-localized Mn/Fe transporter, functions as a primary balancer to regulate Mn distribution in T. urartu under elevated Mn conditions and participates in the intracellular transport and storage of excess Mn as a detoxification mechanism, thereby conferring Mn tolerance”
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