Abstract
Abstract We examined the involvement of nitric oxide (NO) in the protective effect of 24-epicastasterone (EC) and its conjugate with a salicylic acid residue (EC 2-monosalicylate, ECMS) on etiolated wheat seedlings under heat stress. The effect of EC and ECMS at 50 nM concentration, which increased the seedling survival after deleterious heating, led to a transient increase in the NO content in roots. The seedling treatment with NO scavenger PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) completely prevented the stress–protective effect of EC and reduced the protective effect of ECMS. The increase in the content of NO caused by the action of EC and ECMS was noticeably leveled, it may simply be less pronounced in the presence of the sodium tungstate nitrate reductase inhibitor, but not the NO synthase inhibitor aminoguanidine. The increase in the NO content induced by both studied brassinosteroids did not occur when seedlings were treated with a hydrogen peroxide (H2O2) scavenger, dimethylthiourea. The seedling treatment with NO antagonists had practically no effect on the increase of the H2O2 content in roots caused by EC but it decreased this effect of ECMS. It is supposed that the increase of NO content can occur due to the increase of the H2O2 level in the signal pathway induced by EC. The ECMS-induced interactions between NO and H2O2 as signaling mediators can be more complex. The results suggest that NO is involved in the implementation of the EC and ECMS protective effect on seedlings under heat stress.
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Claims 10
Claim · model-written summary
EC increased wheat seedling survival after deleterious heating.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- ECe0
- Outcome
- seedling survivale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of EC and ECMS at 50 nM concentration, which increased the seedling survival after deleterious heating”
t0“Wheat Seedlings”
Claim · model-written summary
ECMS increased wheat seedling survival after deleterious heating.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- ECMS at 50 nM concentratione0
- Outcome
- seedling survivale0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of EC and ECMS at 50 nM concentration, which increased the seedling survival after deleterious heating”
t0“Wheat Seedlings”
Claim · model-written summary
EC and ECMS transiently increased root NO content.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- rootse0
- Treatment
- EC and ECMSt1
- Outcome
- NO contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“led to a transient increase in the NO content in roots”
t0“Wheat Seedlings”
t1“The effect of EC and ECMS at 50 nM concentration”
Claim · model-written summary
PTIO completely prevented EC heat protection.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- NO scavenger PTIOe0
- Outcome
- stress–protective effect of ECe0
- Direction
- preventede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The seedling treatment with NO scavenger PTIO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide) completely prevented the stress–protective effect of EC”
t0“Wheat Seedlings”
Claim · model-written summary
PTIO reduced ECMS heat protection.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- NO scavenger PTIOt1
- Outcome
- protective effect of ECMSe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduced the protective effect of ECMS”
t0“Wheat Seedlings”
t1“The seedling treatment with NO scavenger PTIO”
Claim · model-written summary
Sodium tungstate diminished the EC and ECMS induced NO increase.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- sodium tungstate nitrate reductase inhibitore0
- Outcome
- content of NOe0
- Direction
- less pronouncede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in the content of NO caused by the action of EC and ECMS was noticeably leveled, it may simply be less pronounced in the presence of the sodium tungstate nitrate reductase inhibitor”
t0“Wheat Seedlings”
Claim · model-written summary
Aminoguanidine did not diminish the EC and ECMS induced NO increase.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- NO synthase inhibitor aminoguanidinee0
- Outcome
- increase in the content of NOt1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not the NO synthase inhibitor aminoguanidine”
t0“Wheat Seedlings”
t1“The increase in the content of NO caused by the action of EC and ECMS”
Claim · model-written summary
Dimethylthiourea prevented the NO increase induced by both brassinosteroids.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- dimethylthioureae0
- Outcome
- increase in the NO contente0
- Direction
- did not occure0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in the NO content induced by both studied brassinosteroids did not occur when seedlings were treated with a hydrogen peroxide (H2O2) scavenger, dimethylthiourea.”
t0“Wheat Seedlings”
Claim · model-written summary
NO antagonists had practically no effect on the EC induced root H2O2 increase.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- NO antagonistse0
- Outcome
- increase of the H2O2 content in roots caused by ECe0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The seedling treatment with NO antagonists had practically no effect on the increase of the H2O2 content in roots caused by EC”
t0“Wheat Seedlings”
Claim · model-written summary
NO antagonists decreased the ECMS induced H2O2 increase.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- NO antagonistst1
- Outcome
- this effect of ECMSe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but it decreased this effect of ECMS”
t0“Wheat Seedlings”
t1“The seedling treatment with NO antagonists”
The same questions in other papers
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sodium tungstate · molecular: metabolites · Triticum aestivum (wheat)Results agree
2 papers in the same direction as this paper
- negative
- negative
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- negative
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- positive
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See all 166 papers →biocontrol · physiology: oxidative · Triticum aestivum (wheat)Results agree
2 papers in the same direction as this paper
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.