Paper · 1989 · Journal of the American Society for Horticultural Science

Allelopathic Substances in Asparagus Roots: Extraction, Characterization, and Biological Activity

Jan P. Hazebroek; Stephen A. Garrison; Thomas Gianfagna

18
claims
31
verbatim quotes
19
citations
2
research areas
✓
Open access · cc-by-nc-nd

Abstract

Abstract Aqueous extracts of asparagus ( Asparagus officinalis L.) roots inhibited seed germination in tomato and lettuce, but not in cucumber. The extracts reduced hypocotyl growth in lettuce, shoot growth in asparagus, and inhibited radicle elongation in barley, lettuce, and asparagus. Seedling growth in tomato and two cultivars of wheat were not affected. Inhibition was concentration-dependent. Radicle growth in ‘Grand Rapids’ lettuce was sensitive to an extract concentration as low as 0.05 g dry root tissue/100 ml H 2 O. Asparagus radicles were more sensitive than asparagus shoots. In one experiment, phytotoxicity of crude extract was not altered by autoclaving. Aqueous root extracts of A. racemosis Willd. also inhibited germination and radicle growth in ‘Grand Rapids’ lettuce. A crude extract was purified by solvent partitioning, and charcoal adsorption, cation exchange, and thin-layer chromatography (TLC). A band from the TLC was found to fluoresce under ultraviolet light, react with phenolic-sensitive localization reagents, and inhibit the growth of lettuce and asparagus radicles.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc-nd. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 18

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract inhibited tomato seed germination.

Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse0
Interactor
asparagusAsparaguse0other
Outcome
seed germinatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots inhibited seed germination in tomato and lettuce, but not in cucumber.”

✓ located verbatim in the source · abstract, characters 9–143 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract inhibited lettuce seed germination.

Taxon
lettuceLactuca sativae0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse0
Interactor
asparagusAsparaguse0other
Outcome
seed germinatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots inhibited seed germination in tomato and lettuce, but not in cucumber.”

✓ located verbatim in the source · abstract, characters 9–143 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Asparagus root extract did not inhibit cucumber seed germination.

Taxon
cucumberCucumis sativuse0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse0
Interactor
asparagusAsparaguse0other
Outcome
seed germinatione0
Direction
not ine0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cucumis sativus
Cucumis sativus · TaxonH. Zell · CC BY-SA 3.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots inhibited seed germination in tomato and lettuce, but not in cucumber.”

✓ located verbatim in the source · abstract, characters 9–143 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract reduced lettuce hypocotyl growth.

Taxon
lettuceLactuca sativae0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Interactor
asparagusAsparaguse1other
Outcome
hypocotyl growthe0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The extracts reduced hypocotyl growth in lettuce, shoot growth in asparagus, and inhibited radicle elongation in barley, lettuce, and asparagus.”

✓ located verbatim in the source · abstract, characters 144–288 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract reduced asparagus shoot growth.

Taxon
asparagusAsparaguse0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Outcome
shoot growthe0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The extracts reduced hypocotyl growth in lettuce, shoot growth in asparagus, and inhibited radicle elongation in barley, lettuce, and asparagus.”

✓ located verbatim in the source · abstract, characters 144–288 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract inhibited barley radicle elongation.

Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Interactor
asparagusAsparaguse1other
Outcome
radicle elongatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The extracts reduced hypocotyl growth in lettuce, shoot growth in asparagus, and inhibited radicle elongation in barley, lettuce, and asparagus.”

✓ located verbatim in the source · abstract, characters 144–288 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract inhibited lettuce radicle elongation.

Taxon
lettuceLactuca sativae0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Interactor
asparagusAsparaguse1other
Outcome
radicle elongatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The extracts reduced hypocotyl growth in lettuce, shoot growth in asparagus, and inhibited radicle elongation in barley, lettuce, and asparagus.”

✓ located verbatim in the source · abstract, characters 144–288 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Asparagus root extract inhibited asparagus radicle elongation.

Taxon
asparagusAsparaguse0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Outcome
radicle elongatione0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The extracts reduced hypocotyl growth in lettuce, shoot growth in asparagus, and inhibited radicle elongation in barley, lettuce, and asparagus.”

✓ located verbatim in the source · abstract, characters 144–288 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Asparagus root extract did not affect tomato seedling growth.

Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Interactor
asparagusAsparaguse1other
Outcome
Seedling growthe0
Direction
not affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling growth in tomato and two cultivars of wheat were not affected.”

✓ located verbatim in the source · abstract, characters 289–360 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Asparagus root extract did not affect seedling growth in two wheat cultivars.

Material
two cultivars of wheate0
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Interactor
asparagusAsparaguse1other
Outcome
Seedling growthe0
Direction
not affectede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Seedling growth in tomato and two cultivars of wheat were not affected.”

✓ located verbatim in the source · abstract, characters 289–360 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Extract inhibition depended on concentration.

Taxon
asparagusAsparaguse1
Treatment
Aqueous extracts of asparagus ( Asparagus officinalis L.) rootse1
Outcome
Inhibitione0
Qualifier
concentration-dependente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Inhibition was concentration-dependent.”

✓ located verbatim in the source · abstract, characters 361–400 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Grand Rapids lettuce radicle growth was sensitive at 0.05 g dry root tissue/100 ml H 2 O.

Taxon
lettuceLactuca sativae0
Material
‘Grand Rapids’e0
Treatment
an extract concentration as low as 0.05 g dry root tissue/100 ml H 2 Oe0
Interactor
asparagusAsparaguse1other
Outcome
Radicle growthe0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Radicle growth in ‘Grand Rapids’ lettuce was sensitive to an extract concentration as low as 0.05 g dry root tissue/100 ml H 2 O.”

✓ located verbatim in the source · abstract, characters 401–530 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Asparagus radicles were more sensitive than shoots.

Taxon
AsparagusAsparaguse0
Outcome
radicles were more sensitivee0
Direction
more sensitivee0
Comparator
asparagus shootse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Asparagus radicles were more sensitive than asparagus shoots.”

✓ located verbatim in the source · abstract, characters 531–592 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Autoclaving did not alter crude extract phytotoxicity in one experiment.

Taxon
asparagusAsparaguse1
Treatment
autoclavinge0
Outcome
phytotoxicity of crude extracte0
Direction
not alterede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“In one experiment, phytotoxicity of crude extract was not altered by autoclaving.”

✓ located verbatim in the source · abstract, characters 593–674 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A. racemosis root extracts inhibited Grand Rapids lettuce germination and radicle growth.

Taxon
lettuceLactuca sativae0
Material
‘Grand Rapids’e0
Treatment
Aqueous root extracts of A. racemosis Willd.e0
Interactor
A. racemosis Willd.e0other
Outcome
germination and radicle growthe0
Direction
inhibitede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗

Verbatim

e0

“Aqueous root extracts of A. racemosis Willd. also inhibited germination and radicle growth in ‘Grand Rapids’ lettuce.”

✓ located verbatim in the source · abstract, characters 675–792 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

A TLC band fluoresced under ultraviolet light and reacted with phenolic-sensitive localization reagents.

Taxon
AsparagusAsparagust0
Outcome
fluoresce under ultraviolet light, react with phenolic-sensitive localization reagentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A band from the TLC was found to fluoresce under ultraviolet light, react with phenolic-sensitive localization reagents, and inhibit the growth of lettuce and asparagus radicles.”

✓ located verbatim in the source · abstract, characters 926–1104 · show in text ↑
t0

“Asparagus Roots”

✓ located verbatim in the source · title, characters 27–42 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A TLC band inhibited lettuce radicle growth.

Taxon
lettuceLactuca sativae0
Treatment
A band from the TLCe0
Interactor
asparagusAsparaguse1other
Outcome
growth of lettuce and asparagus radiclese0
Direction
inhibite0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Asparagus
Asparagus · Interactor · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A band from the TLC was found to fluoresce under ultraviolet light, react with phenolic-sensitive localization reagents, and inhibit the growth of lettuce and asparagus radicles.”

✓ located verbatim in the source · abstract, characters 926–1104 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A TLC band inhibited asparagus radicle growth.

Taxon
asparagusAsparaguse0
Treatment
A band from the TLCe0
Outcome
growth of lettuce and asparagus radiclese0
Direction
inhibite0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Asparagus
Asparagus · Taxon · picture of the genus (one of its species)Ольга Лялюк · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A band from the TLC was found to fluoresce under ultraviolet light, react with phenolic-sensitive localization reagents, and inhibit the growth of lettuce and asparagus radicles.”

✓ located verbatim in the source · abstract, characters 926–1104 · show in text ↑
e1

“Aqueous extracts of asparagus ( Asparagus officinalis L.) roots”

✓ located verbatim in the source · abstract, characters 9–72 · show in text ↑

botanical · growth · AsparagusPositive and negative results

3 papers: 1 positive, 2 negative or no effect

1 paper in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
See all 3 papers →

botanical · growth · Hordeum vulgare (barley)Positive and negative results

84 papers: 48 positive, 36 negative or no effect

35 papers in the same direction as this paper · 48 in the opposite direction

  1. positive
  2. positive
  3. positive

80 papers more in this group

See all 84 papers →

botanical · growth · Cucumis sativus (cucumber)Positive and negative results

111 papers: 66 positive, 45 negative or no effect

44 papers in the same direction as this paper · 66 in the opposite direction

  1. positive
  2. positive
  3. positive

107 papers more in this group

See all 111 papers →

botanical · growth · Lactuca sativa (garden lettuce)Positive and negative results

271 papers: 114 positive, 157 negative or no effect

156 papers in the same direction as this paper · 114 in the opposite direction

  1. positive
  2. positive
  3. positive

267 papers more in this group

See all 271 papers →

botanical · growth · Solanum lycopersicum (tomato)Positive and negative results

291 papers: 203 positive, 88 negative or no effect

87 papers in the same direction as this paper · 203 in the opposite direction

  1. positive
  2. positive
  3. positive

287 papers more in this group

See all 291 papers →

botanical · growth · Triticum aestivum (wheat)Positive and negative results

435 papers: 206 positive, 229 negative or no effect

228 papers in the same direction as this paper · 206 in the opposite direction

  1. positive
  2. positive
  3. positive

431 papers more in this group

See all 435 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.