Abstract
Cold is a common abiotic stress that seriously affects plant growth and development. MYB transcription factors are regulatory molecules that play important roles in various biological processes. We have previously demonstrated that SlMYB15 positively regulates cold tolerance in tomato. However, the underlying mechanism of SlMYB15-induced cold tolerance remains largely unexplored. Here, cold-induced SlMYB15 was found to be targeted by Solanum lycopersicum (sly)-miR156e-3p, which was decreased by cold stimulus in tomato. Tomato plants overexpressing sly-MIR156e-3p displayed significant enhancement in susceptibility to cold stress, while silencing of sly-miR156e-3p by an artificial microRNA interference strategy caused tomato plants to be more tolerant to cold. Moreover, both overexpression of SlMYB15 and silencing of sly-miR156e-3p increased the accumulation of ABA. SlMYB15 directly binds to the promoter regions of ABA biosynthesis and signalling genes, SlNCED1 and SlABF4, resulting in enhanced cold tolerance. Further experiments showed that SlMYB15 and sly-miR156e-3p also coordinated the cold tolerance of tomato via the reactive oxygen species (ROS) signalling pathway, as reflected by the increased expression of SlRBOH1, enhanced H2O2 and O2•-accumulation, and amplified activity of antioxidant enzymes in SlMYB15-overexpressing and sly-miR156e-3p-silenced plants. Taken together, our results demonstrate that SlMYB15 targeted by sly-miR156e-3p confers higher survivability to cold stress via ABA and ROS signals. This study provides valuable information for breeding improved crop cultivars better equipped with cold tolerance.
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Claims 10
Claim · model-written summary
Cold induced SlMYB15 and reduced sly-miR156e-3p, which targets SlMYB15, in tomato.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- cold stimuluse0
- Gene
- SlMYB15e0
- Outcome
- sly)-miR156e-3pe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, cold-induced SlMYB15 was found to be targeted by Solanum lycopersicum (sly)-miR156e-3p, which was decreased by cold stimulus in tomato.”
Claim · model-written summary
Overexpression of sly-MIR156e-3p significantly increased tomato susceptibility to cold stress.
- Taxon
- TomatoSolanum lycopersicume0
- Treatment
- overexpressing sly-MIR156e-3pe0
- Gene
- sly-MIR156e-3pe0
- Outcome
- susceptibility to cold stresse0
- Direction
- enhancemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tomato plants overexpressing sly-MIR156e-3p displayed significant enhancement in susceptibility to cold stress”
Claim · model-written summary
Silencing sly-miR156e-3p made tomato plants more tolerant to cold.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of sly-miR156e-3pe0
- Gene
- sly-miR156e-3pe0
- Outcome
- tolerant to colde0
- Direction
- more tolerante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of sly-miR156e-3p by an artificial microRNA interference strategy caused tomato plants to be more tolerant to cold”
Claim · model-written summary
SlMYB15 overexpression increased ABA accumulation.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- overexpression of SlMYB15e0
- Gene
- SlMYB15e0
- Outcome
- accumulation of ABAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both overexpression of SlMYB15 and silencing of sly-miR156e-3p increased the accumulation of ABA”
t0“Tomato SlMYB15 transcription factor targeted by sly-miR156e-3p positively regulates ABA-mediated cold tolerance”
Claim · model-written summary
Silencing sly-miR156e-3p increased ABA accumulation.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- silencing of sly-miR156e-3pe0
- Gene
- sly-miR156e-3pe0
- Outcome
- accumulation of ABAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both overexpression of SlMYB15 and silencing of sly-miR156e-3p increased the accumulation of ABA”
t0“Tomato SlMYB15 transcription factor targeted by sly-miR156e-3p positively regulates ABA-mediated cold tolerance”
Claim · model-written summary
SlMYB15 directly binds promoter regions of SlNCED1 and SlABF4, enhancing cold tolerance.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- SlMYB15e0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlMYB15 directly binds to the promoter regions of ABA biosynthesis and signalling genes, SlNCED1 and SlABF4, resulting in enhanced cold tolerance.”
t0“Tomato SlMYB15 transcription factor targeted by sly-miR156e-3p positively regulates ABA-mediated cold tolerance”
Claim · model-written summary
SlMYB15 overexpression and sly-miR156e-3p silencing increased SlRBOH1 expression in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- SlMYB15-overexpressing and sly-miR156e-3p-silenced plantse0
- Gene
- SlRBOH1e0
- Outcome
- expression of SlRBOH1e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased expression of SlRBOH1, enhanced H2O2 and O2•-accumulation, and amplified activity of antioxidant enzymes in SlMYB15-overexpressing and sly-miR156e-3p-silenced plants”
t0“Tomato SlMYB15 transcription factor targeted by sly-miR156e-3p positively regulates ABA-mediated cold tolerance”
Claim · model-written summary
SlMYB15 overexpression and sly-miR156e-3p silencing enhanced H2O2 and O2•- accumulation in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- SlMYB15-overexpressing and sly-miR156e-3p-silenced plantse0
- Outcome
- H2O2 and O2•-accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhanced H2O2 and O2•-accumulation, and amplified activity of antioxidant enzymes in SlMYB15-overexpressing and sly-miR156e-3p-silenced plants”
t0“Tomato SlMYB15 transcription factor targeted by sly-miR156e-3p positively regulates ABA-mediated cold tolerance”
Claim · model-written summary
SlMYB15 overexpression and sly-miR156e-3p silencing amplified antioxidant enzyme activity in tomato.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- SlMYB15-overexpressing and sly-miR156e-3p-silenced plantse0
- Outcome
- activity of antioxidant enzymese0
- Direction
- amplifiede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amplified activity of antioxidant enzymes in SlMYB15-overexpressing and sly-miR156e-3p-silenced plants”
t0“Tomato SlMYB15 transcription factor targeted by sly-miR156e-3p positively regulates ABA-mediated cold tolerance”
Claim · model-written summary
The authors conclude that SlMYB15 and sly-miR156e-3p coordinate tomato cold tolerance through ROS signalling.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlMYB15e0
- Outcome
- cold tolerancee0
- Qualifier
- via the reactive oxygen species (ROS) signalling pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlMYB15 and sly-miR156e-3p also coordinated the cold tolerance of tomato via the reactive oxygen species (ROS) signalling pathway”
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See all 155 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.