Abstract
Tomato (Solanum lycopersicum) is highly susceptible to both high and low temperatures, which threaten its growth, yield, and quality. Ascorbate peroxidase (APX) plays a pivotal role in plant responses to abiotic stresses. In this study, we unveil the positive involvement of heat- and cold-induced SlAPX2 in bolstering tomato resilience to temperature extremes. Knockout of SlAPX2 using the CRISPR/Cas9 technique exacerbated oxidative stress under heat and cold conditions, as evidenced by reduced Fv/Fm and increased electrolyte leakage (REL), malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) levels. Furthermore, SlAPX2 expression was modulated by abscisic acid (ABA), and the transcription factor ABF4 in the ABA signaling pathway positively regulated SlAPX2 transcription. Using yeast one-hybrid (Y1H) and dual luciferase (LUC) assays, we found that ABF4 directly bound to the SlAPX2 promoter, thereby activating its transcription. Additionally, silencing of SlABF4 compromised tomato’s tolerance to heat or cold. Collectively, these findings reveal a regulatory module, SlABF4–SlAPX2, that enhances tomato tolerance to temperature extremes by detoxifying excessive reactive oxygen species (ROS). This study advances our understanding of ABA-mediated stress responses and highlights the SlABF4–SlAPX2 module as a promising target for breeding temperature-resilient tomato cultivars.
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Claims 12
Claim · model-written summary
SlAPX2 was induced by heat and cold.
- Taxon
- tomato
Solanum lycopersicume0 - Gene
- SlAPX2e0
- Outcome
- heat- and cold-induced SlAPX2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“positive involvement of heat- and cold-induced SlAPX2 in bolstering tomato resilience to temperature extremes.”
Claim · model-written summary
SlAPX2 knockout reduced Fv/Fm under heat and cold.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Knockout of SlAPX2e0
- Gene
- SlAPX2e0
- Outcome
- Fv/Fme0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of SlAPX2 using the CRISPR/Cas9 technique exacerbated oxidative stress under heat and cold conditions, as evidenced by reduced Fv/Fm and increased electrolyte leakage (REL), malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) levels.”
e1“tomato resilience”
Claim · model-written summary
SlAPX2 knockout increased electrolyte leakage under heat and cold.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Knockout of SlAPX2e0
- Gene
- SlAPX2e0
- Outcome
- electrolyte leakage (REL)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of SlAPX2 using the CRISPR/Cas9 technique exacerbated oxidative stress under heat and cold conditions, as evidenced by reduced Fv/Fm and increased electrolyte leakage (REL), malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) levels.”
e1“tomato resilience”
Claim · model-written summary
SlAPX2 knockout increased malondialdehyde content under heat and cold.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Knockout of SlAPX2e0
- Gene
- SlAPX2e0
- Outcome
- malondialdehyde (MDA) contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of SlAPX2 using the CRISPR/Cas9 technique exacerbated oxidative stress under heat and cold conditions, as evidenced by reduced Fv/Fm and increased electrolyte leakage (REL), malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) levels.”
e1“tomato resilience”
Claim · model-written summary
SlAPX2 knockout increased hydrogen peroxide levels under heat and cold.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- Knockout of SlAPX2e0
- Gene
- SlAPX2e0
- Outcome
- hydrogen peroxide (H2O2) levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockout of SlAPX2 using the CRISPR/Cas9 technique exacerbated oxidative stress under heat and cold conditions, as evidenced by reduced Fv/Fm and increased electrolyte leakage (REL), malondialdehyde (MDA) content, and hydrogen peroxide (H2O2) levels.”
e1“tomato resilience”
Claim · model-written summary
ABA modulated SlAPX2 expression.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlAPX2e0
- Compound
- abscisic acid (ABA)e0
- Outcome
- SlAPX2 expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlAPX2 expression was modulated by abscisic acid (ABA)”
e1“tomato resilience”
Claim · model-written summary
ABF4 positively regulated SlAPX2 transcription.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- ABF4e0
- Outcome
- SlAPX2 transcriptione0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription factor ABF4 in the ABA signaling pathway positively regulated SlAPX2 transcription.”
e1“tomato resilience”
Claim · model-written summary
ABF4 directly bound the SlAPX2 promoter.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- ABF4e0
- Outcome
- directly bound to the SlAPX2 promotere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABF4 directly bound to the SlAPX2 promoter, thereby activating its transcription.”
e1“tomato resilience”
Claim · model-written summary
ABF4 binding activated SlAPX2 transcription.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- ABF4e0
- Outcome
- activating its transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABF4 directly bound to the SlAPX2 promoter, thereby activating its transcription.”
e1“tomato resilience”
Claim · model-written summary
SlABF4 silencing compromised tomato heat and cold tolerance.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- silencing of SlABF4e0
- Gene
- SlABF4e0
- Outcome
- tolerance to heat or colde0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of SlABF4 compromised tomato’s tolerance to heat or cold.”
Claim · model-written summary
The SlABF4–SlAPX2 module enhances tomato temperature tolerance by detoxifying excessive ROS.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlABF4–SlAPX2e0
- Outcome
- tolerance to temperature extremese0
- Direction
- enhancese0
- Qualifier
- detoxifying excessive reactive oxygen species (ROS)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlABF4–SlAPX2, that enhances tomato tolerance to temperature extremes by detoxifying excessive reactive oxygen species (ROS).”
Claim · model-written summary
The SlABF4–SlAPX2 module is proposed as a breeding target for temperature-resilient tomato.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- SlABF4–SlAPX2e0
- Outcome
- promising target for breeding temperature-resilient tomato cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SlABF4–SlAPX2 module as a promising target for breeding temperature-resilient tomato cultivars.”
The same questions in other papers
loss of function · disease · Solanum lycopersicum (tomato)Positive and negative results
91 papers in the same direction as this paper · 63 in the opposite direction
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