Abstract
Reactive oxygen species (ROS) have been proposed to function as second messengers in abscisic acid (ABA) signaling in guard cells. However, the question whether ROS production is indeed required for ABA signal transduction in vivo has not yet been addressed, and the molecular mechanisms mediating ROS production during ABA signaling remain unknown. Here, we report identification of two partially redundant Arabidopsis guard cell-expressed NADPH oxidase catalytic subunit genes, AtrbohD and AtrbohF, in which gene disruption impairs ABA signaling. atrbohD/F double mutations impair ABA-induced stomatal closing, ABA promotion of ROS production, ABA-induced cytosolic Ca2+ increases and ABA- activation of plasma membrane Ca2+-permeable channels in guard cells. Exogenous H2O2 rescues both Ca2+ channel activation and stomatal closing in atrbohD/F. ABA inhibition of seed germination and root elongation are impaired in atrbohD/F, suggesting more general roles for ROS and NADPH oxidases in ABA signaling. These data provide direct molecular genetic and cell biological evidence that ROS are rate-limiting second messengers in ABA signaling, and that the AtrbohD and AtrbohF NADPH oxidases function in guard cell ABA signal transduction.
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Claims 11
Claim · model-written summary
AtrbohD and AtrbohF were identified as partially redundant guard-cell-expressed NADPH oxidase genes.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- AtrbohD and AtrbohFe0
- Outcome
- partially redundant Arabidopsis guard cell-expressed NADPH oxidase catalytic subunit genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identification of two partially redundant Arabidopsis guard cell-expressed NADPH oxidase catalytic subunit genes, AtrbohD and AtrbohF”
Claim · model-written summary
atrbohD/F double mutations impaired ABA-induced stomatal closing.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/F double mutationse0
- Treatment
- ABA-inducede0
- Gene
- atrbohD/Fe0
- Outcome
- stomatal closinge0
- Direction
- impaire0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“atrbohD/F double mutations impair ABA-induced stomatal closing, ABA promotion of ROS production, ABA-induced cytosolic Ca2+ increases and ABA- activation of plasma membrane Ca2+-permeable channels in guard cells.”
t0“Arabidopsis”
Claim · model-written summary
atrbohD/F double mutations impaired ABA promotion of ROS production.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/F double mutationse0
- Treatment
- ABA promotione0
- Outcome
- ROS productione0
- Direction
- impaire0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“atrbohD/F double mutations impair ABA-induced stomatal closing, ABA promotion of ROS production, ABA-induced cytosolic Ca2+ increases and ABA- activation of plasma membrane Ca2+-permeable channels in guard cells.”
t0“Arabidopsis”
Claim · model-written summary
atrbohD/F double mutations impaired ABA-induced cytosolic Ca2+ increases.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/F double mutationse0
- Treatment
- ABA-inducede0
- Outcome
- cytosolic Ca2+ increasese0
- Direction
- impaire0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“atrbohD/F double mutations impair ABA-induced stomatal closing, ABA promotion of ROS production, ABA-induced cytosolic Ca2+ increases and ABA- activation of plasma membrane Ca2+-permeable channels in guard cells.”
t0“Arabidopsis”
Claim · model-written summary
atrbohD/F double mutations impaired ABA activation of plasma membrane Ca2+-permeable channels.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/F double mutationse0
- Treatment
- ABA- activatione0
- Outcome
- plasma membrane Ca2+-permeable channelse0
- Direction
- impaire0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“atrbohD/F double mutations impair ABA-induced stomatal closing, ABA promotion of ROS production, ABA-induced cytosolic Ca2+ increases and ABA- activation of plasma membrane Ca2+-permeable channels in guard cells.”
t0“Arabidopsis”
Claim · model-written summary
Exogenous H2O2 rescued Ca2+ channel activation in atrbohD/F.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/Fe0
- Treatment
- Exogenous H2O2e0
- Outcome
- Ca2+ channel activatione0
- Direction
- rescuese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous H2O2 rescues both Ca2+ channel activation and stomatal closing in atrbohD/F.”
t0“Arabidopsis”
Claim · model-written summary
Exogenous H2O2 rescued stomatal closing in atrbohD/F.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/Fe0
- Treatment
- Exogenous H2O2e0
- Outcome
- stomatal closinge0
- Direction
- rescuese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous H2O2 rescues both Ca2+ channel activation and stomatal closing in atrbohD/F.”
t0“Arabidopsis”
Claim · model-written summary
ABA inhibition of seed germination was impaired in atrbohD/F.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/Fe0
- Treatment
- ABAe0
- Outcome
- seed germinatione0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA inhibition of seed germination and root elongation are impaired in atrbohD/F”
t0“Arabidopsis”
Claim · model-written summary
ABA inhibition of root elongation was impaired in atrbohD/F.
- Taxon
- ArabidopsisArabidopsist0
- Material
- atrbohD/Fe0
- Treatment
- ABAe0
- Outcome
- root elongatione0
- Direction
- impairede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA inhibition of seed germination and root elongation are impaired in atrbohD/F”
t0“Arabidopsis”
Claim · model-written summary
The authors concluded that ROS are rate-limiting second messengers in ABA signaling.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- ROSe0
- Outcome
- ABA signalinge0
- Qualifier
- rate-limiting second messengerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS are rate-limiting second messengers in ABA signaling”
t0“Arabidopsis”
Claim · model-written summary
The authors concluded that AtrbohD and AtrbohF NADPH oxidases function in guard cell ABA signal transduction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- AtrbohD and AtrbohF NADPH oxidasese0
- Outcome
- guard cell ABA signal transductione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the AtrbohD and AtrbohF NADPH oxidases function in guard cell ABA signal transduction.”
t0“Arabidopsis”
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.