Abstract
Abstract Vacuolar Phosphate Transporter1 (VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening. Interestingly, here we find that the VPT1 may transport sugar in response to soluble sugar status of fruits. The VvVPT1 protein isolated from grape (Vitis vinifera) berries was tonoplast-localized and contains SPX (Syg1/Pho81/XPR1) and MFS (major facilitator superfamily) domains. Its mRNA expression was significantly increased during fruit ripening and induced by sucrose. Functional analyses based on transient transgenic systems in grape berry showed that VvVPT1 positively regulated berry ripening and significantly affected hexose contents, fruit firmness, and ripening-related gene expression. The VPT1 proteins (Grape VvVPT1, strawberry FaVPT1, and Arabidopsis AtVPT1) all showed low affinity for phosphate verified in yeast system, while they appear different in sugar transport capacity, consistent with fruit sugar status. Thus, our findings reveal a role for VPT1 in fruit ripening, associated to its SPX and MFS domains in direct transport of soluble sugar available into the vacuole, and open potential avenues for genetic improvement in fleshy fruit.
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Claims 12
Claim · model-written summary
Grape VvVPT1 localized to the tonoplast and contained SPX and MFS domains.
Verbatim
e0“The VvVPT1 protein isolated from grape (Vitis vinifera) berries was tonoplast-localized and contains SPX (Syg1/Pho81/XPR1) and MFS (major facilitator superfamily) domains”
Claim · model-written summary
VvVPT1 mRNA expression increased significantly during fruit ripening.
Verbatim
e0“Its mRNA expression was significantly increased during fruit ripening”
t0“grape”
e2“VvVPT1 protein isolated from grape”
Claim · model-written summary
Sucrose induced VvVPT1 mRNA expression.
Not reportedComparator
Verbatim
e0“and induced by sucrose”
t0“grape”
e1“mRNA expression”
e2“VvVPT1 protein isolated from grape”
Claim · model-written summary
VvVPT1 positively regulated berry ripening in transient transgenic grape berries.
- Taxon
- grapee0
- Treatment
- transient transgenic systemse0
- Gene
- VvVPT1e0
- Outcome
- berry ripeninge0
- Direction
- positively regulatede0
Not reportedComparator
Verbatim
e0“Functional analyses based on transient transgenic systems in grape berry showed that VvVPT1 positively regulated berry ripening and significantly affected hexose contents, fruit firmness, and ripening-related gene expression”
Claim · model-written summary
VvVPT1 significantly affected berry hexose contents.
Not reportedComparatorDirection
Verbatim
e0“VvVPT1 positively regulated berry ripening and significantly affected hexose contents, fruit firmness, and ripening-related gene expression”
t0“grape”
Claim · model-written summary
VvVPT1 significantly affected fruit firmness.
Not reportedComparatorDirection
Verbatim
e0“VvVPT1 positively regulated berry ripening and significantly affected hexose contents, fruit firmness, and ripening-related gene expression”
t0“grape”
Claim · model-written summary
VvVPT1 significantly affected ripening-related gene expression.
Not reportedComparatorDirection
Verbatim
e0“VvVPT1 positively regulated berry ripening and significantly affected hexose contents, fruit firmness, and ripening-related gene expression”
t0“grape”
Claim · model-written summary
Grape VvVPT1 showed low phosphate affinity in yeast.
Verbatim
e0“The VPT1 proteins (Grape VvVPT1, strawberry FaVPT1, and Arabidopsis AtVPT1) all showed low affinity for phosphate verified in yeast system”
Claim · model-written summary
Strawberry FaVPT1 showed low phosphate affinity in yeast.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Gene
- FaVPT1e0
- Outcome
- low affinity for phosphatee0
- Setting
- yeast systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The VPT1 proteins (Grape VvVPT1, strawberry FaVPT1, and Arabidopsis AtVPT1) all showed low affinity for phosphate verified in yeast system”
Claim · model-written summary
Arabidopsis AtVPT1 showed low phosphate affinity in yeast.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- AtVPT1e0
- Outcome
- low affinity for phosphatee0
- Setting
- yeast systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The VPT1 proteins (Grape VvVPT1, strawberry FaVPT1, and Arabidopsis AtVPT1) all showed low affinity for phosphate verified in yeast system”
Claim · model-written summary
The three VPT1 proteins differed in sugar transport capacity in a way consistent with fruit sugar status.
Verbatim
e0“they appear different in sugar transport capacity, consistent with fruit sugar status”
e1“Grape”
e2“VvVPT1 protein isolated from grape”
Claim · model-written summary
The authors infer a VPT1 role in ripening through SPX and MFS domains and soluble sugar transport into the vacuole.
Verbatim
e0“our findings reveal a role for VPT1 in fruit ripening, associated to its SPX and MFS domains in direct transport of soluble sugar available into the vacuole”
t0“grape fruits”
The same questions in other papers
sucrose · molecular: expression · VitisPositive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.