Abstract
Although it is well established that nitrogen (N) deficiency induces leaf senescence, the molecular mechanism of N deficiency-induced leaf senescence remains largely unknown. Here, we show that an abscisic acid (ABA)-responsive NAC transcription factor (TF) is involved in N deficiency-induced leaf senescence. The overexpression of MdNAC4 led to increased ABA levels in apple calli by directly activating the transcription of the ABA biosynthesis gene MdNCED2. In addition, MdNAC4 overexpression promoted N deficiency-induced leaf senescence. Further investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene (SAG) MdSAG39 and upregulated its expression. Interestingly, the function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ABA. Furthermore, we identified an interaction between the ABA receptor protein MdPYL4 and the MdNAC4 protein. Moreover, MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiency-induced leaf senescence. These findings suggest that ABA plays a central role in N deficiency-induced leaf senescence and that MdPYL4 interacts with MdNAC4 to enhance the response of the latter to N deficiency, thus promoting N deficiency-induced leaf senescence. In conclusion, our results provide new insight into how MdNAC4 regulates N deficiency-induced leaf senescence.
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Claims 9
Claim · model-written summary
MdNAC4 overexpression increased ABA levels in apple calli.
- Taxon
- apple
Malus domesticae1 - Organ / stage
- callie1
- Treatment
- overexpression of MdNAC4e1
- Outcome
- ABA levelse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The overexpression of MdNAC4 led to increased ABA levels in apple calli”
Claim · model-written summary
MdNAC4 directly activates transcription of the ABA biosynthesis gene MdNCED2 in apple calli.
- Taxon
- appleMalus domesticae2
- Treatment
- overexpression of MdNAC4e2
- Gene
- MdNCED2e2
- Outcome
- transcription of the ABA biosynthesis gene MdNCED2e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The overexpression of MdNAC4 led to increased ABA levels in apple calli by directly activating the transcription of the ABA biosynthesis gene MdNCED2”
Claim · model-written summary
MdNAC4 overexpression promoted N deficiency-induced leaf senescence in apple.
- Taxon
- appleMalus domesticat1
- Treatment
- MdNAC4 overexpressione3
- Outcome
- N deficiency-induced leaf senescencee3
- Direction
- promotede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e3“In addition, MdNAC4 overexpression promoted N deficiency-induced leaf senescence”
Claim · model-written summary
MdNAC4 directly bound the promoter of the senescence-associated gene MdSAG39 in apple.
- Taxon
- appleMalus domesticat1
- Gene
- MdSAG39e4
- Outcome
- bound the promoter of the senescence-associated gene (SAG) MdSAG39e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e4“Further investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene (SAG) MdSAG39 and upregulated its expression”
Claim · model-written summary
MdNAC4 upregulated the expression of MdSAG39 in apple.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e4“Further investigation showed that MdNAC4 directly bound the promoter of the senescence-associated gene (SAG) MdSAG39 and upregulated its expression”
Claim · model-written summary
The function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ABA.
- Taxon
- appleMalus domesticat1
- Treatment
- in the presence of ABAe5
- Compound
- ABAe5
- Outcome
- function of MdNAC4 in promoting N deficiency-induced leaf senescencee5
- Direction
- enhancede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e5“Interestingly, the function of MdNAC4 in promoting N deficiency-induced leaf senescence was enhanced in the presence of ABA”
Claim · model-written summary
An interaction between MdPYL4 and MdNAC4 was identified in apple.
- Taxon
- appleMalus domesticat1
- Gene
- MdPYL4e6
- Outcome
- interaction between the ABA receptor protein MdPYL4 and the MdNAC4 proteine6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e6“Furthermore, we identified an interaction between the ABA receptor protein MdPYL4 and the MdNAC4 protein”
Claim · model-written summary
MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiency-induced leaf senescence in apple.
- Taxon
- appleMalus domesticat1
- Gene
- MdPYL4e7
- Outcome
- function similar to that of MdNAC4 in ABA-mediated N deficiency-induced leaf senescencee7
- Comparator
- MdNAC4e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e7“Moreover, MdPYL4 showed a function similar to that of MdNAC4 in ABA-mediated N deficiency-induced leaf senescence”
Claim · model-written summary
ABA plays a central role in N deficiency-induced leaf senescence in apple.
- Taxon
- appleMalus domesticat1
- Compound
- ABAe0
- Outcome
- N deficiency-induced leaf senescencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“ABA biosynthesis in apple”
e0“These findings suggest that ABA plays a central role in N deficiency-induced leaf senescence”
The same questions in other papers
(+)-abscisic acid · quality: postharvest · Malus domestica (apple)Results agree
7 papers in the same direction as this paper
- positive
- positive
- positive
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.