Abstract
High NaCl (200 mM) increases the transcription of phospholipase Dδ (PLDδ) in roots and leaves of the salt-resistant woody species Populus euphratica. We isolated a 1138 bp promoter fragment upstream of the translation initiation codon of PePLDδ. A promoter–reporter construct, PePLDδ-pro::GUS, was introduced into Arabidopsis plants (Arabidopsis thaliana) to demonstrate the NaCl-induced PePLDδ promoter activity in root and leaf tissues. Mass spectrometry analysis of DNA pull-down-enriched proteins in P. euphratica revealed that PeGLABRA3, a basic helix–loop–helix transcription factor, was the target transcription factor for binding the promoter region of PePLDδ. The PeGLABRA3 binding to PePLDδ-pro was further verified by virus-induced gene silencing, luciferase reporter assay (LRA), yeast one-hybrid assay, and electrophoretic mobility shift assay (EMSA). In addition, the PeGLABRA3 gene was cloned and overexpressed in Arabidopsis to determine the function of PeGLABRA3 in salt tolerance. PeGLABRA3-overexpressed Arabidopsis lines (OE1 and OE2) had a greater capacity to scavenge reactive oxygen species (ROS) and to extrude Na+ under salinity stress. Furthermore, the EMSA and LRA results confirmed that PeGLABRA3 interacted with the promoter of AtPLDδ in transgenic plants. The upregulated AtPLDδ in PeGLABRA3-transgenic lines resulted in an increase in phosphatidic acid species under no-salt and saline conditions. We conclude that PeGLABRA3 activated AtPLDδ transcription under salt stress by binding to the AtPLDδ promoter region, conferring Na+ and ROS homeostasis control via signaling pathways mediated by PLDδ and phosphatidic acid.
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Claims 10
Claim · model-written summary
High NaCl increased PLDδ transcription in Populus roots and leaves.
- Taxon
- Populus euphratica
Populus euphraticae0 - Organ / stage
- roots and leavese0
- Treatment
- High NaCl (200 mM)e0
- Gene
- phospholipase Dδ (PLDδ)e0
- Outcome
- transcription of phospholipase Dδ (PLDδ)e0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High NaCl (200 mM) increases the transcription of phospholipase Dδ (PLDδ) in roots and leaves of the salt-resistant woody species Populus euphratica.”
Claim · model-written summary
NaCl induced PePLDδ promoter activity in Arabidopsis root and leaf tissue.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- root and leaf tissuese0
- Treatment
- NaCle0
- Gene
- PePLDδe0
- Outcome
- PePLDδ promoter activitye0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A promoter–reporter construct, PePLDδ-pro::GUS, was introduced into Arabidopsis plants (Arabidopsis thaliana) to demonstrate the NaCl-induced PePLDδ promoter activity in root and leaf tissues.”
Claim · model-written summary
PeGLABRA3 was identified as a factor binding the PePLDδ promoter.
- Taxon
- P. euphraticaPopulus euphraticae0
- Gene
- PeGLABRA3e0
- Outcome
- binding the promoter region of PePLDδe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mass spectrometry analysis of DNA pull-down-enriched proteins in P. euphratica revealed that PeGLABRA3, a basic helix–loop–helix transcription factor, was the target transcription factor for binding the promoter region of PePLDδ.”
Claim · model-written summary
Several assays verified binding of PeGLABRA3 to PePLDδ promoter.
- Taxon
- Populus euphraticaPopulus euphraticat0
- Gene
- PeGLABRA3e0
- Outcome
- PeGLABRA3 binding to PePLDδ-proe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The PeGLABRA3 binding to PePLDδ-pro was further verified by virus-induced gene silencing, luciferase reporter assay (LRA), yeast one-hybrid assay, and electrophoretic mobility shift assay (EMSA).”
t0“Populus euphratica”
Claim · model-written summary
PeGLABRA3 overexpression increased Arabidopsis capacity to scavenge reactive oxygen species (ROS).
- Taxon
- ArabidopsisArabidopsise0
- Material
- OE1 and OE2e0
- Treatment
- PeGLABRA3-overexpressede0
- Gene
- PeGLABRA3e0
- Outcome
- scavenge reactive oxygen species (ROS)e0
- Direction
- greatere0
- Qualifier
- under salinity stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeGLABRA3-overexpressed Arabidopsis lines (OE1 and OE2) had a greater capacity to scavenge reactive oxygen species (ROS) and to extrude Na+ under salinity stress.”
Claim · model-written summary
PeGLABRA3 overexpression increased Arabidopsis capacity to extrude Na+.
- Taxon
- ArabidopsisArabidopsise0
- Material
- OE1 and OE2e0
- Treatment
- PeGLABRA3-overexpressede0
- Gene
- PeGLABRA3e0
- Outcome
- extrude Na+e0
- Direction
- greatere0
- Qualifier
- under salinity stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeGLABRA3-overexpressed Arabidopsis lines (OE1 and OE2) had a greater capacity to scavenge reactive oxygen species (ROS) and to extrude Na+ under salinity stress.”
Claim · model-written summary
PeGLABRA3 interacted with the AtPLDδ promoter in transgenic plants.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PeGLABRA3e0
- Outcome
- interacted with the promoter of AtPLDδe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, the EMSA and LRA results confirmed that PeGLABRA3 interacted with the promoter of AtPLDδ in transgenic plants.”
t0“Arabidopsis plants (Arabidopsis thaliana)”
Claim · model-written summary
Upregulated AtPLDδ increased phosphatidic acid species in transgenic lines.
- Taxon
- ArabidopsisArabidopsist0
- Material
- PeGLABRA3-transgenic linese0
- Gene
- AtPLDδe0
- Outcome
- increase in phosphatidic acid speciese0
- Qualifier
- under no-salt and saline conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The upregulated AtPLDδ in PeGLABRA3-transgenic lines resulted in an increase in phosphatidic acid species under no-salt and saline conditions.”
t0“Arabidopsis plants (Arabidopsis thaliana)”
Claim · model-written summary
The authors conclude PeGLABRA3 binding activates AtPLDδ transcription during salt stress.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PeGLABRA3e0
- Outcome
- AtPLDδ transcriptione0
- Qualifier
- by binding to the AtPLDδ promoter regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We conclude that PeGLABRA3 activated AtPLDδ transcription under salt stress by binding to the AtPLDδ promoter region, conferring Na+ and ROS homeostasis control via signaling pathways mediated by PLDδ and phosphatidic acid.”
t0“Arabidopsis plants (Arabidopsis thaliana)”
Claim · model-written summary
The authors conclude PLDδ and phosphatidic acid signaling controls Na+ and ROS homeostasis.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PLDδe0
- Compound
- phosphatidic acide0
- Outcome
- Na+ and ROS homeostasis controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We conclude that PeGLABRA3 activated AtPLDδ transcription under salt stress by binding to the AtPLDδ promoter region, conferring Na+ and ROS homeostasis control via signaling pathways mediated by PLDδ and phosphatidic acid.”
t0“Arabidopsis plants (Arabidopsis thaliana)”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.