Abstract
The bacterial leaf streak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a continuous threat to rice cultivation leading to substantial yield losses and socio-economic implications. Iron ions are essential mineral nutrients for plant growth, but little information is available on how these ions influence the immune mechanism of rice against Xoc. Hence, we investigate the role of the MYB transcriptional repressor, OsMYBxoc1, in modulating rice resistance by controlling the transport of iron ions. The overexpression of OsMYBxoc1 significantly increased rice resistance, and the OsMYBxoc1 inhibiting expressing lines and the knockout mutants were opposite. Suppressing OsMYBxoc1 expression dampened the pathogen-associated molecular pattern (PAMP)-induced immune response. We identified that OsMYBxoc1 specifically binds to the OsNRAMP5 promoter and represses the transcription of OsNRAMP5. OsNRAMP5, a negative regulator of rice resistance to BLS, possesses metal ion transport activity. Inhibition of OsMYBxoc1 expression increased the iron ions content in rice. The ion-dependent H2O2 scavenging enzyme activity was increased in plants with OsMYBxoc1 inhibited expression and OsNRAMP5 overexpression. We found that iron ions promote Xoc infection and interferes with reactive oxygen species production induced by Xoc. The type III effector XopAK directly inhibits OsMYBxoc1 transcription, indicating that pathogen may promote its own proliferation by relieving the restriction of iron ion transport in plants. In addition, iron could complement the phenotype of RS105_ΔXopAK mutant strain's pathogenicity defects, further confirming that iron utilization by Xoc may be XopAK dependent. In conclusion, our study unveils a novel mechanism through which OsMYBxoc1 modulates rice resistance by regulating iron accumulation, whereas the Xoc can accumulate iron ions by secreting the effector XopAK to promote its own infection.
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Claims 13
Claim · model-written summary
OsMYBxoc1 overexpression significantly increased rice resistance.
- Taxon
- rice
Oryza sativae0 - Treatment
- overexpression of OsMYBxoc1e0
- Gene
- OsMYBxoc1e0
- Outcome
- rice resistancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of OsMYBxoc1 significantly increased rice resistance”
Claim · model-written summary
OsMYBxoc1 inhibited lines and knockout mutants showed the opposite resistance response.
Not reportedTaxonComparator
Verbatim
e0“the OsMYBxoc1 inhibiting expressing lines and the knockout mutants were opposite”
Claim · model-written summary
Suppressing OsMYBxoc1 dampened the PAMP-induced immune response.
- Treatment
- Suppressing OsMYBxoc1 expressione0
- Gene
- OsMYBxoc1e0
- Outcome
- pathogen-associated molecular pattern (PAMP)-induced immune responsee0
- Direction
- dampenede0
Not reportedTaxonComparator
Verbatim
e0“Suppressing OsMYBxoc1 expression dampened the pathogen-associated molecular pattern (PAMP)-induced immune response.”
Claim · model-written summary
OsMYBxoc1 binds the OsNRAMP5 promoter and represses transcription.
Not reportedTaxon
Verbatim
e0“OsMYBxoc1 specifically binds to the OsNRAMP5 promoter and represses the transcription of OsNRAMP5”
Claim · model-written summary
OsNRAMP5 negatively regulates rice resistance to BLS.
- Taxon
- riceOryza sativae0
- Gene
- OsNRAMP5e0
- Outcome
- rice resistance to BLSe0
- Direction
- negativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsNRAMP5, a negative regulator of rice resistance to BLS, possesses metal ion transport activity.”
Claim · model-written summary
OsNRAMP5 has metal ion transport activity.
Not reportedTaxon
Verbatim
e0“OsNRAMP5, a negative regulator of rice resistance to BLS, possesses metal ion transport activity.”
Claim · model-written summary
Inhibiting OsMYBxoc1 increased iron ion content in rice.
- Taxon
- riceOryza sativae0
- Treatment
- Inhibition of OsMYBxoc1 expressione0
- Gene
- OsMYBxoc1e0
- Outcome
- iron ions contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of OsMYBxoc1 expression increased the iron ions content in rice.”
Claim · model-written summary
Ion-dependent H2O2 scavenging activity increased when OsMYBxoc1 expression was inhibited.
- Treatment
- OsMYBxoc1 inhibited expressione0
- Gene
- OsMYBxoc1e0
- Outcome
- ion-dependent H2O2 scavenging enzyme activitye0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“The ion-dependent H2O2 scavenging enzyme activity was increased in plants with OsMYBxoc1 inhibited expression and OsNRAMP5 overexpression.”
Claim · model-written summary
Ion-dependent H2O2 scavenging activity increased with OsNRAMP5 overexpression.
- Treatment
- OsNRAMP5 overexpressione0
- Gene
- OsNRAMP5e0
- Outcome
- ion-dependent H2O2 scavenging enzyme activitye0
- Direction
- increasede0
Not reportedTaxonComparator
Verbatim
e0“The ion-dependent H2O2 scavenging enzyme activity was increased in plants with OsMYBxoc1 inhibited expression and OsNRAMP5 overexpression.”
Claim · model-written summary
Iron ions promoted Xoc infection.
Not reportedTaxonComparator
Verbatim
e0“iron ions promote Xoc infection”
Claim · model-written summary
Iron ions interfered with Xoc-induced reactive oxygen species production.
- Treatment
- iron ionse0
- Interactor
- Xoce0pathogen
- Outcome
- reactive oxygen species productione0
- Direction
- interferese0
Not reportedTaxonComparator
Verbatim
e0“iron ions promote Xoc infection and interferes with reactive oxygen species production induced by Xoc”
Claim · model-written summary
The XopAK effector directly inhibits OsMYBxoc1 transcription.
- Gene
- OsMYBxoc1e0
- Outcome
- OsMYBxoc1 transcriptione0
- Direction
- inhibitse0
- Qualifier
- type III effector XopAKe0
Not reportedTaxon
Verbatim
e0“The type III effector XopAK directly inhibits OsMYBxoc1 transcription”
Claim · model-written summary
Iron complemented the pathogenicity defect of the RS105_ΔXopAK mutant strain.
- Treatment
- irone0
- Interactor
- RS105_ΔXopAK mutant straine0pathogen
- Outcome
- pathogenicity defectse0
- Direction
- complemente0
Not reportedTaxonComparator
Verbatim
e0“iron could complement the phenotype of RS105_ΔXopAK mutant strain's pathogenicity defects”
The same questions in other papers
iron atom · physiology: oxidative · Oryza sativa (rice)Positive and negative results
3 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
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