Abstract
Background: Grapevine (Vitis vinifera L.) is a globally significant fruit crop known for its nutritional and health benefits, including its role in preventing chronic diseases such as cancer and cardiovascular disorders. The bioactive compounds in grapes contribute to these benefits. Bioactive content changes due to grapevine variety and environmental conditions necessitate ongoing research. In vitro propagation provides a controlled environment for grapevine cultivation, minimizing disease risk and ensuring consistent quality. This study investigates the potential of Nadsoniella nigra (Nn), an Antarctic yeast, as a natural alternative to synthetic auxins like Indole-3-butyric acid (IBA) in grapevine micro cutting propagation. Objective: To evaluate the effectiveness of the culture liquid of Nn as a natural alternative to auxin-class hormones, using IBA as an example, for promoting rooting in grapevine microcuttings and to assess its impact on chlorophyll content, vitamin C levels, and sugar concentrations. Materials and Methods: In vitro propagation of grapevine (Vitis vinifera L.) cultivar 'Karmarhyt' was performed using half-strength Woody Plant Medium (WPM). Nn culture liquid was prepared at concentrations of 0.5, 1.0, 1.5, and 2.0 ml/L, and IBA was used at equivalent concentrations. Microcuttings were cultured on media supplemented with these treatments, with a control group maintained without growth regulators. Rooting was evaluated after 4 weeks by measuring rooting percentage, number of roots per shoot, and root length. Chlorophyll a (Chl a) and Chlorophyll b (Chl b) levels, vitamin C content, and sugar concentrations were assessed. Statistical analysis was performed using standard error and t-tests. Results: Rooting performance of grapevine microcuttings varied with treatment. Nn culture liquid promoted rooting in a dose-dependent manner, with the highest values observed at 2.0 ml/L (97.0% rooting, 7.2 roots, 13.8 cm root length). IBA treatment resulted in peak rooting at 1.0 mg/L (100% rooting) but decreased at higher concentrations, accompanied by callus formation. Chlorophyll content was higher in Nn culture liquid treated microcuttings, with a maximum total chlorophyll of 2.20 mg/g at 2.0 mg/L, compared to a peak of 2.05 mg/g in IBA-treated microcuttings at 1.0 mg/L. Sugar and vitamin C content increased with Nn culture liquid concentrations, whereas IBA showed a less consistent effect. Conclusion: Nn culture liquid was an effective natural alternative to IBA for promoting rooting in grapevine microcuttings. It improved rooting efficiency, chlorophyll content, and increased sugar and vitamin C levels, while IBA led to growth inhibition and callus formation at higher concentrations. Nn culture liquid demonstrated consistent benefits, suggesting potential for sustainable viticulture. As a result of the conducted research, it was established that the mixture of secondary metabolites contained in the liquid culture medium of Nn possesses high biological activity and can be recommended for use in plant biotechnology in vitro to promote the growth of both underground and above-ground parts. Future research could explore its use in propagating other plant species. Keywords: ´Karmrahyut´, grapevine, culture liquid of Nadsoniella nigra. in vitro propagation, functional foods
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Claims 10
Claim · model-written summary
Nn culture liquid promoted rooting of grapevine microcuttings dose-dependently, with the highest rooting percentage of 97.0% at 2.0 ml/L.
- Treatment
- Nn culture liquide1
- Outcome
- rootinge1
- Direction
- higheste1
- Value
- 97.0% rootinge1
- Qualifier
- dose-dependent mannere1
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“Nn culture liquid promoted rooting in a dose-dependent manner, with the highest values observed at 2.0 ml/L (97.0% rooting, 7.2 roots, 13.8 cm root length)”
Claim · model-written summary
At 2.0 ml/L Nn culture liquid gave the highest number of roots per microcutting, 7.2 roots.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“Nn culture liquid promoted rooting in a dose-dependent manner, with the highest values observed at 2.0 ml/L (97.0% rooting, 7.2 roots, 13.8 cm root length)”
Claim · model-written summary
At 2.0 ml/L Nn culture liquid gave the highest root length, 13.8 cm.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“Nn culture liquid promoted rooting in a dose-dependent manner, with the highest values observed at 2.0 ml/L (97.0% rooting, 7.2 roots, 13.8 cm root length)”
Claim · model-written summary
IBA treatment gave peak rooting of 100% at 1.0 mg/L in grapevine microcuttings.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“IBA treatment resulted in peak rooting at 1.0 mg/L (100% rooting) but decreased at higher concentrations, accompanied by callus formation”
Claim · model-written summary
Rooting with IBA decreased at concentrations higher than 1.0 mg/L.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“IBA treatment resulted in peak rooting at 1.0 mg/L (100% rooting) but decreased at higher concentrations, accompanied by callus formation”
Claim · model-written summary
Callus formation accompanied the decreased rooting at higher IBA concentrations.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“IBA treatment resulted in peak rooting at 1.0 mg/L (100% rooting) but decreased at higher concentrations, accompanied by callus formation”
Claim · model-written summary
Chlorophyll content was higher in Nn culture liquid-treated microcuttings, with a maximum total chlorophyll of 2.20 mg/g at 2.0 mg/L, compared with a peak of 2.05 mg/g in IBA-treated microcuttings at 1.0 mg/L.
- Treatment
- Nn culture liquide1
- Outcome
- Chlorophyll contente1
- Direction
- highere1
- Value
- 2.20 mg/ge1
- Qualifier
- 2.05 mg/ge1
- Comparator
- IBA-treated microcuttingse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“Chlorophyll content was higher in Nn culture liquid treated microcuttings, with a maximum total chlorophyll of 2.20 mg/g at 2.0 mg/L, compared to a peak of 2.05 mg/g in IBA-treated microcuttings at 1.0 mg/L”
Claim · model-written summary
Sugar and vitamin C contents of grapevine microcuttings increased with increasing Nn culture liquid concentrations.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“Sugar and vitamin C content increased with Nn culture liquid concentrations, whereas IBA showed a less consistent effect”
Claim · model-written summary
IBA showed a less consistent effect than Nn culture liquid on sugar and vitamin C contents.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“grapevine (Vitis vinifera L.)”
e1“Sugar and vitamin C content increased with Nn culture liquid concentrations, whereas IBA showed a less consistent effect”
Claim · model-written summary
The authors recommend the Nn culture liquid for use in plant biotechnology in vitro to promote growth of both underground and above-ground parts.
- Outcome
- promote the growth of both underground and above-ground partse1
Not reportedTaxon
Verbatim
e1“can be recommended for use in plant biotechnology in vitro to promote the growth of both underground and above-ground parts”
The same questions in other papers
indole-3-butyric acid · growth · VitisResults differ
32 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
30 papers more in this group
See all 34 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.