Abstract
• BTH treatment induced the disease resistance against Colletotrichum musae in banana fruit . • BTH treatment delayed fruit ripening and induced the transcripts related to disease resistance. • WRKY transcription factors played important role in the disease resistance . • BTH treatment increased the flavonoid metabolites after C. musae infection. Benzothiadiazole (BTH) is a plant resistance inducer that effectively delays ripening and senescence in fruits. To understand its possible mechanism, transcriptomics and metabolomics analyses were performed on banana ( Musa spp.) fruit treated with BTH after their inoculation with the fungal pathogen Colletotrichum musae. A total of 1,747 differentially expressed genes (DEGs) were identified, and 1,160 were up- and 587 downregulated between the BTH and control conditions. These DEGs were highly enriched in metabolic pathways related to disease resistance, such as phenylpropanoid biosynthesis, flavonoids, and starch and sucrose metabolism. A metabolome analysis identified 138 differentially accumulated metabolites (DAMs) of which 73 were upregulated and 65 downregulated between the BTH and control conditions. Flavonoids were significantly upregulated DAMs. Transcriptome and metabolome analyses showed that the lignin biosynthesis and flavonoid biosynthesis pathways played important roles in the disease resistance of banana fruit induced by BTH by upregulating the expression of PAL, 4CL, PER, CHS, FLS , and FL3H . Moreover, WRKY transcription factors (TFs) also played key roles in this process of resistance by mediating genes related to disease resistance, such as PR1, PR1c, TLPH, PTI5, and other genes. Our results suggested that the BTH treatment significantly changed the expression of transcripts and metabolites related to the hormone signaling, secondary metabolism, disease resistance, and key TFs, which induced the disease resistance and delayed the ripening process. The insights into these molecular mechanisms also provide potential targets for genetic or chemical approaches to further enhance fruit quality and resistance.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 12
Claim · model-written summary
BTH induced banana fruit resistance against Colletotrichum musae.
- Taxon
- bananae0
- Treatment
- BTH treatmente0
- Interactor
- Colletotrichum musae
-_Anthracnose_caused_by_Colletotrichum_musae.jpg?width=60)
Colletotrichum musaee0pathogen - Outcome
- disease resistancee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
-_Anthracnose_caused_by_Colletotrichum_musae.jpg?width=500)
Verbatim
e0“BTH treatment induced the disease resistance against Colletotrichum musae in banana fruit”
Claim · model-written summary
BTH delayed fruit ripening.
Not reportedComparator
Verbatim
e0“BTH treatment delayed fruit ripening”
t0“banana”
Claim · model-written summary
BTH induced transcripts related to disease resistance.
- Taxon
- bananat0
- Treatment
- BTH treatmente0
- Outcome
- transcripts related to disease resistancee0
- Direction
- inducede0
Not reportedComparator
Verbatim
e0“BTH treatment delayed fruit ripening and induced the transcripts related to disease resistance”
t0“banana”
Claim · model-written summary
BTH and control differed in 1,747 gene transcripts, with 1,160 upregulated.
Verbatim
e0“A total of 1,747 differentially expressed genes (DEGs) were identified, and 1,160 were up- and 587 downregulated between the BTH and control conditions”
t0“banana”
Claim · model-written summary
Among differentially expressed genes, 587 were downregulated under BTH.
Verbatim
e0“587 downregulated between the BTH and control conditions”
t0“banana”
Claim · model-written summary
Differentially expressed genes were enriched in disease resistance related pathways.
Verbatim
e0“These DEGs were highly enriched in metabolic pathways related to disease resistance, such as phenylpropanoid biosynthesis, flavonoids, and starch and sucrose metabolism”
t0“banana”
Claim · model-written summary
Metabolomics identified 138 differentially accumulated metabolites.
Verbatim
e0“A metabolome analysis identified 138 differentially accumulated metabolites (DAMs)”
t0“banana”
Claim · model-written summary
BTH increased 73 metabolites relative to control.
Verbatim
e0“73 were upregulated and 65 downregulated between the BTH and control conditions”
t0“banana”
Claim · model-written summary
BTH decreased 65 metabolites relative to control.
Verbatim
e0“65 downregulated between the BTH and control conditions”
t0“banana”
Claim · model-written summary
Flavonoids were significantly upregulated.
Verbatim
e0“Flavonoids were significantly upregulated DAMs”
t0“banana”
Claim · model-written summary
BTH induced resistance through lignin and flavonoid biosynthesis and expression of PAL, 4CL, PER, CHS, FLS and FL3H.
Verbatim
e0“lignin biosynthesis and flavonoid biosynthesis pathways played important roles in the disease resistance of banana fruit induced by BTH by upregulating the expression of PAL, 4CL, PER, CHS, FLS , and FL3H”
Claim · model-written summary
WRKY transcription factors mediated disease resistance genes during banana fruit resistance.
Verbatim
e0“WRKY transcription factors (TFs) also played key roles in this process of resistance by mediating genes related to disease resistance, such as PR1, PR1c, TLPH, PTI5”
t0“banana”
The same questions in other papers
acibenzolar-S-methyl · quality: postharvest · MusaResults agree
1 paper in the same direction as this paper
- positive
acibenzolar-S-methyl · molecular: expression · MusaResults agree
3 papers in the same direction as this paper
- positive
- positive
- positive
acibenzolar-S-methyl · Colletotrichum musae · MusaResults agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.