Abstract
ABSTRACT Members of the cyclic nucleotide‐gated channel (CNGC) proteins are reportedly involved in a variety of biotic and abiotic responses and stomatal movement. However, it is unknown if and how a single member could regulate multiple responses. Here we characterized three closely related CNGC genes in rice, OsCNGC14 , OsCNGC15 and OsCNGC16 , to determine whether they function in multiple abiotic stresses. The loss‐of‐function mutants of each of these three genes had reduced calcium ion (Ca 2+ ) influx and slower stomatal closure in response to heat, chilling, drought and the stress hormone abscisic acid (ABA). These mutants also had reduced tolerance to heat, chilling and drought compared with the wild‐type. Conversely, overexpression of OsCNGC16 led to a more rapid stomatal closure response to stresses and enhanced tolerance to heat, chilling and drought. The tight association of stomatal closure and stress tolerance strongly suggests that tolerance to multiple abiotic stresses conferred by these OsCNGC genes results at least partially from their regulation of stomatal movement. In addition, physical interactions were observed among the three OsCNGC proteins but not with a distantly related CNGC, suggesting the formation of hetero‐oligomers among themselves. This study unveils the crucial role of OsCNGC14, 15 and 16 proteins in stomatal response and tolerance to multiple stresses, suggesting a mechanism of tolerance to multiple stresses that involves calcium influx and stomatal movement regulation.
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Claims 10
Claim · model-written summary
Loss of each of three OsCNGC genes reduced calcium influx in response to heat, chilling, drought and ABA.
- Taxon
- rice
Oryza sativat0 - Treatment
- loss‐of‐function mutants of each of these three genese0
- Outcome
- calcium ion (Ca 2+ ) influxe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The loss‐of‐function mutants of each of these three genes had reduced calcium ion (Ca 2+ ) influx and slower stomatal closure in response to heat, chilling, drought and the stress hormone abscisic acid (ABA).”
t0“rice”
Claim · model-written summary
Loss of each of three OsCNGC genes slowed stomatal closure in response to heat, chilling, drought and ABA.
- Taxon
- riceOryza sativat0
- Treatment
- loss‐of‐function mutants of each of these three genese0
- Outcome
- stomatal closuree0
- Direction
- slowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The loss‐of‐function mutants of each of these three genes had reduced calcium ion (Ca 2+ ) influx and slower stomatal closure in response to heat, chilling, drought and the stress hormone abscisic acid (ABA).”
t0“rice”
Claim · model-written summary
The mutants had reduced heat, chilling and drought tolerance versus wild type.
- Taxon
- riceOryza sativat0
- Treatment
- These mutantse0
- Outcome
- tolerance to heat, chilling and droughte0
- Direction
- reducede0
- Comparator
- wild‐typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These mutants also had reduced tolerance to heat, chilling and drought compared with the wild‐type.”
t0“rice”
Claim · model-written summary
OsCNGC16 overexpression accelerated stress induced stomatal closure.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsCNGC16e0
- Gene
- OsCNGC16e0
- Outcome
- stomatal closure responsee0
- Direction
- more rapide0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsCNGC16 led to a more rapid stomatal closure response to stresses”
t0“rice”
Claim · model-written summary
OsCNGC16 overexpression enhanced heat, chilling and drought tolerance.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsCNGC16e0
- Gene
- OsCNGC16e0
- Outcome
- tolerance to heat, chilling and droughte0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsCNGC16 led to a more rapid stomatal closure response to stresses and enhanced tolerance to heat, chilling and drought.”
t0“rice”
Claim · model-written summary
Stomatal closure and stress tolerance were tightly associated.
- Taxon
- riceOryza sativat0
- Gene
- OsCNGC genese0
- Outcome
- tight association of stomatal closure and stress tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The tight association of stomatal closure and stress tolerance strongly suggests that tolerance to multiple abiotic stresses conferred by these OsCNGC genes results at least partially from their regulation of stomatal movement.”
t0“rice”
Claim · model-written summary
Authors suggest OsCNGC stress tolerance partly arises from regulation of stomatal movement.
- Taxon
- riceOryza sativat0
- Gene
- OsCNGC genese0
- Outcome
- tolerance to multiple abiotic stressese0
- Qualifier
- regulation of stomatal movemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tolerance to multiple abiotic stresses conferred by these OsCNGC genes results at least partially from their regulation of stomatal movement.”
t0“rice”
Claim · model-written summary
The three OsCNGC proteins physically interact with one another.
- Taxon
- riceOryza sativat0
- Gene
- OsCNGC proteinse0
- Outcome
- physical interactions were observed among the three OsCNGC proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“physical interactions were observed among the three OsCNGC proteins but not with a distantly related CNGC”
t0“rice”
Claim · model-written summary
The three OsCNGC proteins did not physically interact with a distant CNGC.
- Taxon
- riceOryza sativat0
- Gene
- OsCNGC proteinse0
- Outcome
- but not with a distantly related CNGCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“physical interactions were observed among the three OsCNGC proteins but not with a distantly related CNGC”
t0“rice”
Claim · model-written summary
The observed protein interactions suggest hetero-oligomer formation.
- Taxon
- riceOryza sativat0
- Outcome
- formation of hetero‐oligomers among themselvese0
- Qualifier
- among themselvese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting the formation of hetero‐oligomers among themselves.”
t0“rice”
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