Abstract
Elaeagnus mollis Diels, is a rare and endangered woody plant endemic to China, which is listed on the IUCN Red List. In the natural state, the viability of its seeds declines very rapidly, which is the key to its endangered status, but the mechanism of E. mollis seed viability decline is still unclear. In order to explore the physiological and molecular mechanism of viability decline of E. mollis seeds, this study used fresh seeds as a control to compare and analyze the changes of seed vitality, antioxidant system, transcription and metabolomics, when seeds were stored for 1 and 3 months at room temperature. The viability of E. mollis seed decreased continuously after 1 month and 3 months of storage. The activities of superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR), ascorbate (AsA), and glutathione (GSH) decreased significantly, while catalase (CAT) activity increased gradually during the decline of seed viability. Transcriptomic results showed that a total of 801 differentially expressed genes (DEGs) were identified between fresh and 1-month-stored seeds, while 1,524 were identified between fresh and 3-month-stored seeds. Among them, the expression of CAT, MDHAR, GSH and GR were consistent with the results of physiological indicators. Moreover, WRKY, C3H, bZIP, B3, bHLH, NAC and AP2 / ERF-ERF transcription factors are important in regulating seed viability. Metabolomics results showed that the types of differential accumulated metabolites (DAMs) during viability decline were mainly flavonoids, amino acids and derivatives, and phenolic acids. The combined analysis results of transcriptomics and metabolomics further showed that DEGs and DAMs associated with viability were co-enriched in flavonoid biosynthesis and tryptophan metabolism pathways. Also identified were 22 key antioxidant genes, including CAT, ALDH, CHS and C4H, which were identified as participating in the changes of seed viability. This also illustrated that the metabolic pathways of flavonoid biosynthesis and tryptophan metabolism were involved in regulating the decline of seed viability by acting on the antioxidant system. These findings provide new insights into the mechanism of seed viability decline of E. mollis.
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Claims 16
Claim · model-written summary
E. mollis seed viability declined after one and three months at room temperature.
- Taxon
- E. mollisElaeagnus mollise0
- Treatment
- 1 month and 3 months of storagee0
- Outcome
- viabilitye0
- Direction
- decreased continuouslye0
Not reportedComparator
Verbatim
e0“The viability of E. mollis seed decreased continuously after 1 month and 3 months of storage.”
Claim · model-written summary
superoxide dismutase (SOD) activity decreased significantly during seed viability decline.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Organ / stage
- seede0
- Outcome
- superoxide dismutase (SOD)e0
- Direction
- decreased significantlye0
Verbatim
e0“The activities of superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR), ascorbate (AsA), and glutathione (GSH) decreased significantly, while catalase (CAT) activity increased gradually during the decline of seed viability.”
t0“Elaeagnus mollis diels seed”
Claim · model-written summary
monodehydroascorbate reductase (MDHAR) activity decreased significantly during seed viability decline.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Organ / stage
- seede0
- Outcome
- monodehydroascorbate reductase (MDHAR)e0
- Direction
- decreased significantlye0
Verbatim
e0“The activities of superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR), ascorbate (AsA), and glutathione (GSH) decreased significantly, while catalase (CAT) activity increased gradually during the decline of seed viability.”
t0“Elaeagnus mollis diels seed”
Claim · model-written summary
ascorbate (AsA) activity decreased significantly during seed viability decline.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Organ / stage
- seede0
- Outcome
- ascorbate (AsA)e0
- Direction
- decreased significantlye0
Verbatim
e0“The activities of superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR), ascorbate (AsA), and glutathione (GSH) decreased significantly, while catalase (CAT) activity increased gradually during the decline of seed viability.”
t0“Elaeagnus mollis diels seed”
Claim · model-written summary
glutathione (GSH) activity decreased significantly during seed viability decline.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Organ / stage
- seede0
- Outcome
- glutathione (GSH)e0
- Direction
- decreased significantlye0
Verbatim
e0“The activities of superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR), ascorbate (AsA), and glutathione (GSH) decreased significantly, while catalase (CAT) activity increased gradually during the decline of seed viability.”
t0“Elaeagnus mollis diels seed”
Claim · model-written summary
Catalase activity increased gradually during seed viability decline.
Verbatim
e0“The activities of superoxide dismutase (SOD), monodehydroascorbate reductase (MDHAR), ascorbate (AsA), and glutathione (GSH) decreased significantly, while catalase (CAT) activity increased gradually during the decline of seed viability.”
t0“Elaeagnus mollis diels seed”
Claim · model-written summary
801 DEGs were identified between fresh and 1-month-stored seeds.
Verbatim
e0“Transcriptomic results showed that a total of 801 differentially expressed genes (DEGs) were identified between fresh and 1-month-stored seeds, while 1,524 were identified between fresh and 3-month-stored seeds.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
1,524 DEGs were identified between fresh and 3-month-stored seeds.
Verbatim
e0“Transcriptomic results showed that a total of 801 differentially expressed genes (DEGs) were identified between fresh and 1-month-stored seeds, while 1,524 were identified between fresh and 3-month-stored seeds.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
CAT, MDHAR, GSH and GR expression was consistent with physiological indicators.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Gene
- CAT, MDHAR, GSH and GRe0
- Outcome
- expressione0
- Comparator
- physiological indicatorse0
Not reportedDirection
Verbatim
e0“Among them, the expression of CAT, MDHAR, GSH and GR were consistent with the results of physiological indicators.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
WRKY, C3H, bZIP transcription factors were implicated in regulation of seed viability.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Gene
- WRKY, C3H, bZIPe0
- Outcome
- seed viabilitye0
Verbatim
e0“Moreover, WRKY, C3H, bZIP, B3, bHLH, NAC and AP2 / ERF-ERF transcription factors are important in regulating seed viability.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
B3, bHLH, NAC transcription factors were implicated in regulation of seed viability.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Gene
- B3, bHLH, NACe0
- Outcome
- seed viabilitye0
Verbatim
e0“Moreover, WRKY, C3H, bZIP, B3, bHLH, NAC and AP2 / ERF-ERF transcription factors are important in regulating seed viability.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
AP2 / ERF-ERF transcription factors were implicated in regulation of seed viability.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Gene
- AP2 / ERF-ERFe0
- Outcome
- seed viabilitye0
Verbatim
e0“Moreover, WRKY, C3H, bZIP, B3, bHLH, NAC and AP2 / ERF-ERF transcription factors are important in regulating seed viability.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
Differentially accumulated metabolites during viability decline were mainly flavonoids, amino acids and derivatives, and phenolic acids.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Organ / stage
- seedt0
- Outcome
- flavonoids, amino acids and derivatives, and phenolic acidse0
Verbatim
e0“Metabolomics results showed that the types of differential accumulated metabolites (DAMs) during viability decline were mainly flavonoids, amino acids and derivatives, and phenolic acids.”
t0“Elaeagnus mollis diels seed”
Claim · model-written summary
Viability-associated DEGs and DAMs were co-enriched in flavonoid biosynthesis and tryptophan metabolism pathways.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Gene
- DEGse0
- Compound
- DAMse0
- Outcome
- co-enriched in flavonoid biosynthesis and tryptophan metabolism pathwayse0
Not reportedDirection
Verbatim
e0“The combined analysis results of transcriptomics and metabolomics further showed that DEGs and DAMs associated with viability were co-enriched in flavonoid biosynthesis and tryptophan metabolism pathways.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
Twenty-two key antioxidant genes, including CAT, ALDH, CHS and C4H, were identified as participating in seed viability changes.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Gene
- CAT, ALDH, CHS and C4He0
- Outcome
- key antioxidant genese0
- Value
- 22e0
Verbatim
e0“Also identified were 22 key antioxidant genes, including CAT, ALDH, CHS and C4H, which were identified as participating in the changes of seed viability.”
t0“Elaeagnus mollis diels”
Claim · model-written summary
Flavonoid biosynthesis and tryptophan metabolism pathways were interpreted as regulating seed viability decline through the antioxidant system.
- Taxon
- Elaeagnus mollis dielsElaeagnus mollist0
- Outcome
- decline of seed viabilitye0
- Qualifier
- by acting on the antioxidant systeme0
Verbatim
e0“This also illustrated that the metabolic pathways of flavonoid biosynthesis and tryptophan metabolism were involved in regulating the decline of seed viability by acting on the antioxidant system.”
t0“Elaeagnus mollis diels”