Abstract
Plant lectins have a significant impact on the defense against pathogens and insect attacks. The jacalin-related lectin OsMbl1 from rice (Oryza sativa L.) has been reported to play a crucial role in pattern-triggered immunity (PTI). However, the underlying mechanism remains unclear. In this study, we identified a GDSL-like lipase, OsGdsl1, that interacts with OsMbl1 both in vitro and in vivo. The OsGdsl1 protein, which has lipase activity, is localized in the lipid bodies and apoplast. The expression of OsGDSL1 is modulated upon exposure to Magnaporthe oryzae (M. oryzae) or plant hormones. Deletion of the OsGDSL1 gene not only improved the resistance of rice to M. oryzae, but also led to an increased ROS burst after chitin treatments. The expression of some pathogenesis-related (PR) genes was upregulated in the mutants. We also found that OsMbl1 inhibited the lipase activity of OsGdsl1 during infection with M. oryzae. Overall, our results suggest that OsGdsl1 negatively regulates rice immunity to M. oryzae infection by downregulating ROS bursts and PR gene expressions, while its lipase activity, which is inhibited by OsMbl1, contributes to the enhancement of rice innate immunity during M. oryzae infection.
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Claims 9
Claim · model-written summary
OsGdsl1 interacts with OsMbl1 both in vitro and in vivo.
- Taxon
- rice
Oryza sativat0 - Gene
- OsGdsl1e1
- Outcome
- interacts with OsMbl1e1
- Qualifier
- both in vitro and in vivoe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we identified a GDSL-like lipase, OsGdsl1, that interacts with OsMbl1 both in vitro and in vivo”
t0“Rice Blast Susceptibility”
Claim · model-written summary
The OsGdsl1 protein has lipase activity and is localized in lipid bodies and the apoplast.
- Taxon
- riceOryza sativat0
- Gene
- OsGdsl1 proteine2
- Outcome
- lipase activitye2
- Qualifier
- localized in the lipid bodies and apoplaste2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“The OsGdsl1 protein, which has lipase activity, is localized in the lipid bodies and apoplast”
t0“Rice Blast Susceptibility”
Claim · model-written summary
OsGDSL1 expression is modulated by exposure to Magnaporthe oryzae or plant hormones.
- Taxon
- riceOryza sativat0
- Treatment
- exposure to Magnaporthe oryzaee3
- Interactor
- Magnaporthe oryzae
Pyricularia oryzaee3pathogen - Gene
- OsGDSL1e3
- Outcome
- expression of OsGDSL1e3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“The expression of OsGDSL1 is modulated upon exposure to Magnaporthe oryzae (M. oryzae) or plant hormones”
t0“Rice Blast Susceptibility”
Claim · model-written summary
Deletion of OsGDSL1 improved rice resistance to M. oryzae.
- Taxon
- riceOryza sativat0
- Treatment
- Deletion of the OsGDSL1 genee4
- Interactor
- M. oryzaePyricularia oryzaee4pathogen
- Gene
- OsGDSL1e4
- Outcome
- resistance of rice to M. oryzaee4
- Direction
- improvede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Deletion of the OsGDSL1 gene not only improved the resistance of rice to M. oryzae”
t0“Rice Blast Susceptibility”
Claim · model-written summary
Deletion of OsGDSL1 increased the ROS burst after chitin treatments.
- Taxon
- riceOryza sativat0
- Treatment
- Deletion of the OsGDSL1 genee4
- Gene
- OsGDSL1e4
- Outcome
- ROS burste5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Deletion of the OsGDSL1 gene not only improved the resistance of rice to M. oryzae”
e5“led to an increased ROS burst after chitin treatments”
t0“Rice Blast Susceptibility”
Claim · model-written summary
Pathogenesis-related gene expression was upregulated in the OsGDSL1 mutants.
- Taxon
- riceOryza sativat0
- Material
- mutantse6
- Outcome
- expression of some pathogenesis-related (PR) genese6
- Direction
- upregulatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“The expression of some pathogenesis-related (PR) genes was upregulated in the mutants”
t0“Rice Blast Susceptibility”
Claim · model-written summary
OsMbl1 inhibited the lipase activity of OsGdsl1 during M. oryzae infection.
- Taxon
- riceOryza sativat0
- Interactor
- M. oryzaePyricularia oryzaee7pathogen
- Gene
- OsMbl1e7
- Outcome
- lipase activity of OsGdsl1e7
- Qualifier
- during infection with M. oryzaee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“We also found that OsMbl1 inhibited the lipase activity of OsGdsl1 during infection with M. oryzae”
t0“Rice Blast Susceptibility”
Claim · model-written summary
OsGdsl1 negatively regulates rice immunity to M. oryzae by downregulating ROS bursts and PR gene expression.
- Taxon
- riceOryza sativat0
- Gene
- OsGdsl1e7
- Outcome
- rice immunity to M. oryzae infectione7
- Qualifier
- by downregulating ROS bursts and PR gene expressionse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“OsGdsl1 negatively regulates rice immunity to M. oryzae infection by downregulating ROS bursts and PR gene expressions”
t0“Rice Blast Susceptibility”
Claim · model-written summary
The lipase activity of OsGdsl1, inhibited by OsMbl1, contributes to enhanced rice innate immunity during M. oryzae infection.
- Taxon
- riceOryza sativat0
- Gene
- OsMbl1e6
- Outcome
- enhancement of rice innate immunitye6
- Qualifier
- lipase activitye6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“its lipase activity, which is inhibited by OsMbl1, contributes to the enhancement of rice innate immunity during M. oryzae infection”
t0“Rice Blast Susceptibility”
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See all 67 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.