Abstract
Plants have evolved a complex regulatory network to cope with heat stress (HS), which includes microRNAs (miRNAs). However, the roles of the entire miRNA biogenesis machinery in HS responses remain unclear. Here, we show that HS induces the majority of miRNAs primarily through the enhanced nuclear localization of HYPONASTIC LEAVES 1 (HYL1), rather than by upregulating MIR gene transcription in Arabidopsis (Arabidopsis thaliana). Disruption of miRNA biogenesis increases plant susceptibility to HS. We also demonstrate that HYL1 phosphorylation modulates its nuclear localization during HS, which is critical for miRNA induction and thermotolerance. MAP KINASE3 (MPK3) phosphorylates and stabilizes the phosphatase C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1), while CPL1 inhibits MPK3 activity, creating a feedback loop that regulates HYL1 phosphorylation. Disruption of MPK3 function results in increased nuclear HYL1 levels and miRNA production, conferring enhanced HS tolerance to mpk3 mutants. These findings reveal a mechanism by which plants enhance miRNA biogenesis during HS, offering insights into the regulatory networks that govern plant thermotolerance.
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Claims 7
Claim · model-written summary
Heat stress induces most miRNAs primarily through enhanced nuclear HYL1 localization.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- HSe0
- Gene
- HYPONASTIC LEAVES 1 (HYL1)e0
- Outcome
- majority of miRNAse0
- Direction
- inducese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HS induces the majority of miRNAs primarily through the enhanced nuclear localization of HYPONASTIC LEAVES 1 (HYL1), rather than by upregulating MIR gene transcription in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Disrupting miRNA biogenesis increased heat stress susceptibility.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- Disruption of miRNA biogenesise0
- Outcome
- plant susceptibility to HSe0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of miRNA biogenesis increases plant susceptibility to HS”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
HYL1 phosphorylation modulates nuclear localization during heat stress.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- HYL1e0
- Outcome
- nuclear localizatione0
- Qualifier
- phosphorylatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HYL1 phosphorylation modulates its nuclear localization during HS”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MPK3 phosphorylates and stabilizes CPL1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- MAP KINASE3 (MPK3)e0
- Outcome
- C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MAP KINASE3 (MPK3) phosphorylates and stabilizes the phosphatase C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1)”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
CPL1 inhibits MPK3 activity in a feedback loop.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- CPL1e0
- Outcome
- MPK3 activitye0
- Direction
- inhibitse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CPL1 inhibits MPK3 activity, creating a feedback loop that regulates HYL1 phosphorylation”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MPK3 disruption increased nuclear HYL1 and miRNA production.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- Disruption of MPK3 functione0
- Gene
- MPK3e0
- Outcome
- nuclear HYL1 levels and miRNA productione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of MPK3 function results in increased nuclear HYL1 levels and miRNA production”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MPK3 mutants had enhanced heat stress tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Material
- mpk3 mutantse0
- Outcome
- HS tolerancee0
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“conferring enhanced HS tolerance to mpk3 mutants”
e1“Arabidopsis (Arabidopsis thaliana)”
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